The effectiveness of flow cytometric sorting of human sperm (MicroSort) for influencing a child’s sex

Abstract Background: Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X-vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X-or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Herein we report the effectiveness of flow cytometric sorting of human sperm and its use in human ART procedures