36 research outputs found
Bacillus sphaericus Binary Toxin Elicits Host Cell Autophagy as a Response to Intoxication
Bacillus sphaericus strains that produce the binary toxin (Bin) are highly toxic to Culex and Anopheles mosquitoes, and have been used since the late 1980s as a biopesticide for the control of these vectors of infectious disease agents. The Bin toxin produced by these strains targets mosquito larval midgut epithelial cells where it binds to Cpm1 (Culex pipiens maltase 1) a digestive enzyme, and causes severe intracellular damage, including a dramatic cytoplasmic vacuolation. The intoxication of mammalian epithelial MDCK cells engineered to express Cpm1 mimics the cytopathologies observed in mosquito enterocytes following Bin ingestion: pore formation and vacuolation. In this study we demonstrate that Bin-induced vacuolisation is a transient phenomenon that affects autolysosomes. In addition, we show that this vacuolisation is associated with induction of autophagy in intoxicated cells. Furthermore, we report that after internalization, Bin reaches the recycling endosomes but is not localized either within the vacuolating autolysosomes or within any other degradative compartment. Our observations reveal that Bin elicits autophagy as the cell's response to intoxication while protecting itself from degradation through trafficking towards the recycling pathways
Pharmacologie, purification et reconstitution du canal sodium dependant du voltage
SIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc
Voltage-dependent sodium channels in susceptible and pyrethroid-resistant Drosophila strains
International audienc
Identification and in-situ localization of subunit of voltage-sensitive sodium channels from Drosophila using polyclonal antibodies
International audienc
Immunological characterization of DSC1 in several Drosophila strains
International audienc
Immunohistolocalization of cells in which sodium channel para genes are expressed
International audienc
A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC delta-endotoxin on Spodoptera littoralis and Bombyx mori
International audienc
In vivo toxicity and receptor binding properties of the Bacillus thuringiensis cryic delta-endotoxin on Spodoptera littoralis and Bombyx mori
National audienc
Transcription analysis of the para gene by in situ hybridization and immunological characterization of its expression product in wild-type and mutant strains of Drosophila
International audienc
DSC1 is the major sodium channel expressed in the peripheral nervous system of adult Drosophila
National audienc