CloVR: A virtual machine for automated and portable sequence analysis from the desktop using cloud computing

Next-generation sequencing technologies have decentralized sequence acquisition, increasing the demand for new bioinformatics tools that are easy to use, portable across multiple platforms, and scalable for high-throughput applications. Cloud computing platforms provide on-demand access to computing infrastructure over the Internet and can be used in combination with custom built virtual machines to distribute pre-packaged with pre-configured software. We describe the Cloud Virtual Resource, CloVR, a new desktop application for push-button automated sequence analysis that can utilize cloud computing resources. CloVR is implemented as a single portable virtual machine (VM) that provides several automated analysis pipelines for microbial genomics, including 16S, whole genome and metagenome sequence analysis. The CloVR VM runs on a personal computer, utilizes local computer resources and requires minimal installation, addressing key challenges in deploying bioinformatics workflows. In addition CloVR supports use of remote cloud computing resources to improve performance for large-scale sequence processing. In a case study, we demonstrate the use of CloVR to automatically process next-generation sequencing data on multiple cloud computing platforms. The CloVR VM and associated architecture lowers the barrier of entry for utilizing complex analysis protocols on both local single- and multi-core computers and cloud systems for high throughput data processing.https://doi.org/10.1186/1471-2105-12-35

The vote that made the president. [electronic resource]/

Electronic text and image data.Mode of access: Internet

The electoral votes of 1876 [electronic resource]: who should count them, what should be counted, and the remedy for a wrong count./

An introduction p. [ix]-liv extracted from "Presidential counts", is inserted before t.-p.Electronic text and image data.Mode of access: Internet

Draft outlines of an international code.

Mode of access: Internet

The electoral votes of 1876: who should count them, what should be counted, and the remedy for a wrong count./ By David Dudley Field.

22 p., 1 l ; 24 cm.An introduction p. [ix]-liv extracted from "Presidential counts", is inserted before t.-p.Electronic text and image data. Ann Arbor, Mich. : University of Michigan, Digital Library Initiatives, 1996. Includes both TIFF files and keyword searchable text. [Making of America] This volume is made possible by a grant from the Andrew W. Mellon Foundation

The vote that made the president.: by David Dudley Field.

22 p ; 23 cm.Electronic text and image data. Ann Arbor, Mich. : University of Michigan, Digital Library Initiatives, 1996. Includes both TIFF files and keyword searchable text. [Making of America] This volume is made possible by a grant from the Andrew W. Mellon Foundation

The electoral votes of 1876: who should count them, what should be counted, and the remedy for a wrong count.

Mode of access: Internet