Structural Genomics of Minimal Organisms: Pipeline and Results

The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93percent of all soluble proteins of M. genitalium is avail able. This chapter summarizes the approaches taken by the authors' center

IDENTIFYING THE MYSTERIOUS EGRET SOURCES: SIGNATURES OF POLAR CAP PULSAR MODELS

The advent of the next generation of gamma-ray experiments, led by GLAST, AGILE, INTEGRAL and a host of atmospheric Čerenkov telescopes coming on line in the next few years, will enable ground-breaking discoveries relating to the presently enigmatic set of EGRET/CGRO UID galactic sources that have yet to find definitive identifications. Pulsars are principal candidates for such sources, and many are expected to be detected by GLAST, some that are radio-selected, like most of the present EGRET/Comptel pulsars, and perhaps even more that are detected via independent pulsation searches. At this juncture, it is salient to outline the principal predictions of pulsar models that might aid identification of gamma-ray sources, and moreover propel subsequent interpretation of their properties. This review summarizes relevant characteristics of the polar cap model, emphasizing where possible distinctions from the competing outer gap model. Foremost among these considerations are the hard X-ray to gamma-ray spectral shape, high energy cutoffs and pulse profiles, and how these characteristics generally depend on pulsar period and period derivative, as well as observational viewing angle. The polar cap model exhibits definitive signatures that will be readily tested by the detections of GLAST and other experiments, thereby establishing cogent observational diagnostics. The paper focuses on different classes of pulsars that might define agendas and parameter regimes for blind gamma-ray pulsation searches; examples include the highly-magnetized ones that are currently quite topical in astrophysics. 1 2 1