Adenosine deaminase and its isoenzyme as a diagnostic marker in tubercular Plural effusion

Mortality and residual morbidity is quit high in tubercular meningitis particular in developing countries like India. Several investigations have been developed for early diagnosis but this sill poses a lot of diagnostic problem and financial burden. So, the present study was performed to search a quick and definite investigation for easy and rapid diagnosis of tubercular meningitis and pleural fluid ADA and its isoenzymes activity was selected to differentiate between tubercular and non-tubercular exudative pleural effusion. Among 87 exudative pleural effusion patients, 55 were tubercular, 16 were empyemic and 16 were malignant selected. Pleural fluid total ADA and ADA2 activity were measured and compared within the different types of exudative pleural effusion. The pleural fluid ADA activity had no significant different among exudative pleural effusion. But when ADA2 was selected it can efficiently differentiated tubercular form non-tubercular origin. ADA2 activity than total ADA in pleural fluid are elevated in suspected tubercular pleural effusion cases and it is a simple, rapid, inexpensive and has got definite diagnostic value. It is thus a useful biochemical marker for the early diagnosis of tubercular pleural effusion

EFFECT OF FREQUENT BLOOD DONATION ON IRON STATUS OF BLOOD DONORS IN BURDWAN, WEST BENGAL, INDIA

Background: Anaemia was reported as one of the most important reasons for blood donor deferral and very frequent blood donation will have a negative impact on the health of the donor and quality of donated blood products. Aim: The purpose of the study was to assess the influence of frequency of blood donation on iron stores of the donors. Method: The study population were divided into five groups. Forty one were first time (control group), Thirty-eight were ≤ 10 times, thirty-two were 11-20 times, twenty seven were 21-50 times and seventeen were > 50 time blood donors. Pre-donation blood haemoglobin and serum ferritin assessment was done in each individual. Result: Serum ferritin of < 10 times/4years were not significantly reduced than first time healthy donors but > 10 times/4years donors onwards iron status were significantly reduced (p>0.001) progressively as frequency of donation increased and individuals potentially at risk for developing iron deficiency. Conclusion: It is the conclusion of this study that increased frequency of blood donation reduced the iron stores of donors. Therefore, volunteers who undergo repeated blood donation and professional donors should be advised on eating balanced diet as well as receive nutritional care especially in terms of iron supplementation. Key Words: Blood donation, Serum ferritin, Iron deficiency

Blood Vitamin D concentration In Adult Asthmatic Patients

Background: There has been growing interest in vitamin D insufficiency as a predisposing factor for allergy development based on immunoregulatory properties and epidemiological studies. Little is known about vitamin D in adult asthma patients or its association with asthma severity. Aim: The present study is to assess the relationship between vitamin D status and asthma in adult population. Methods: A cross sectional study including 622 asthmatic adults aged older than 18 years and 617 adults healthy control aged older than 18 years assessing the relationship between serum 25 hydroxy vitamin D levels. Result: The difference between mean vitamin D levels in the asthmatic group 24.96 ± 5.58 ng/ml and the healthy control group 32.39 ± 5.28 ng/ml was found to be statistically significant (p < 0.001) and more interestingly there was significant correlation between low 25 OHD level and the severity of asthma. Conclusion: Reduced vitamin D levels are highly prevalent in adult asthmatic patients and are associated severity of the disease

FORMATION OF A SCALE FOR EVALUATION OF THESIS QUALITY IN MD CURRICULUM IN WEST BENGAL

Back ground: Thesis  is an obligatory part of Medical post graduate or post doctoral courses under West Bengal University of Health Sciences.  The purpose of this thesis work is to make the doctors familiar with research methodology. There is no procedure or scale to evaluate the quality of thesis works in our state. Aim:Formation of a scale for evaluation of thesis work & using this scale evaluation of ongoing thesis works of this medical university. Procedure: At first we develop a scale containing five factors  Format, Study design,Knowledge of the post graduate trainee,New ideas,Literature reviewed and validation of that  scale was done.With the help of this validated scale we have evaluated 135 thesis works of out going post graduate medical students. Result: After evaluation it was found that most of the thesis work fall in the excellent category.Very few are exceptional as well as good or acceptable.The scale also indicated that the weak areas of the thesis works. Conclusion: Our scale will indicate the clear picture of quality of a research work including strong and weak areas. This fact will help us in future to plan a better research work

RENAL PROFILE IN AMIKACIN THERAPY IN NEONATAL SEPTICEMIA

Background: Neonatal sepsis is the most common cause of neonatal deaths followed by extreme prematurity and birth asphyxia. In our hospital settings, a case of septicemia is treated with aminoglycosides (AG) combined with third generation cephalosporins. This is the standard guidelines of treatment of septicaemia.Though the duration of therapy should be 5-7days for most probable sepsis and 14 days for culture positive cases but as the cost of therapy is high, and the laboratory procedures for monitoring such duration of therapy is not possible in our hospital , short duration of 5-day therapy is practiced. Among the AGs, amikacin being extensively studied highly preferred agent because of its broadest spectrum, resistance to inactivating enzymes , least lysosomal damage, better tolerability profile and also low cost of therapy. The present piece of work aims at assessing any change in the renal profile of the babies being treated with 5-day amikacin therapy. Materials and methods: Serum Creatinine,urea,potassium and 24 hours urinary Creatinine clearance were estimated on day 1(before initiation of therapy)and on day 3, day 5 of the septicemic neonates.These parameters were selected to assess the status of renal function of the affected neonates. Results and conclusion :There was no significant changes in renal function with the regimen

Comparison Between Serum hsCRP and LDL Cholesterol for Search of a Better Predictor for Ischemic Heart Disease

Acute myocardial Infarction is one of the major causes of morbidity & mortality in world and atherosclerosis is the major cause of ischemic heart disease. In order to determine the better clinical marker of atherosclerosis, we estimated serum low-density lipoprotein (LDL-C) and high sensitivity C-reactive protein (hsCRP). Hundred patients of myocardial infarction and 100 controls irrespective of age and sex were studied for these parameters over a period of 2 years. The statistical analysis showed that the serum hsCRP was significantly raised in myocardial infarction cases than controls (P < 0.01) but LDL-C was not (P > 0.05). We conclude that the serum hsCRP has better predictive value for risk of atherosclerosis