Protein Expression and Purification of Human Hormone Sensitive Lipase

The purpose of this project was to develop a new product, purified human hormone sensitive lipase (HSL), for Blue Sky Biotech's product line. This was accomplished by cloning the HSL gene by gene synthesis, amplifying the annealed templates by PCR, cloning the amplicons into a plasmid in E. coli, packaging the gene into baculovirus, expressing the gene in cultured insect cells, and purifying the protein by affinity chromatography using histidine and biotin tags contained in the engineered vector. The results indicate that HSL has been purified and remains biologically active. Blue Sky Biotech will be able to sell this product to laboratories currently researching lipolysis pathways involved in diseases such as obesity, diabetes, and hyperlipidemia