Bark Extract of Lantana camara in 1M HCl as Green Corrosion Inhibitor for Mild Steel

Lantana camara, an invasive species that adversely affects habitant, bioregions and environment has been studied as corrosion inhibitor. Methanolic extract of barks of Lantana camara in 1 M hydrochloric acid was tested as corrosion inhibitor on mild steel using potentiodynamic polarization technique. The corrosion inhibition efficiency of extract varied with concentration of extract and immersion of time. The inhibition was found to increase with increase in concentration of the extract. The polarization behavior of mild steel revealed that maximum inhibition efficiency is 97.33 % and 89.93 % respectively in the 1000 and 200 ppm concentration of the inhibitor respectively. The results showed that the extract of the barks of Lantana camara served as a mixed type inhibitor

Evaluation of potential role of R-loop and G-quadruplex DNA in the fragility of c-MYC during chromosomal translocation associated with Burkitt\u27s lymphoma

t(8;14) translocation is the hallmark of Burkitt\u27s lymphoma and results in c-MYC deregulation. During the translocation, c-MYC gene on chromosome 8 gets juxtaposed to the Ig switch regions on chromosome 14. Although the promoter of c-MYC has been investigated for its mechanism of fragility, little is known about other c-MYC breakpoint regions. We have analyzed the translocation break points at the exon 1/intron 1 of c-MYC locus from patients with Burkitt\u27s lymphoma. Results showed that the breakpoint region, when present on a plasmid, could fold into an R-loop confirmation in a transcription-dependent manner. Sodium bisulfite modification assay revealed significant single-strandedness on chromosomal DNA of Burkitt\u27s lymphoma cell line, Raji, and normal lymphocytes, revealing distinct R-loops covering up to 100 bp region. Besides, ChIP-DRIP analysis reveals that the R-loop antibody can bind to the breakpoint region. Further, we show the formation of stable parallel intramolecular G-quadruplex on non-template strand of the genome. Finally, incubation of purified AID in vitro or overexpression of AID within the cells led to enhanced mutation frequency at the c-MYC breakpoint region. Interestingly, anti-γH2AX can bind to DSBs generated at the c-MYC breakpoint region within the cells. The formation of R-loop and G-quadruplex was found to be mutually exclusive. Therefore, our results suggest that AID can bind to the single-stranded region of the R-loop and G4 DNA, leading to the deamination of cytosines to uracil and induction of DNA breaks in one of the DNA strands, leading to double-strand break, which could culminate in t(8;14) chromosomal translocation

Alkaloid of <i>Rhynchostylis retusa</i> as Green Inhibitor for Mild Steel Corrosion in 1 M H₂SO₄ Solution

Alkaloids are aromatic hydrocarbons with nitrogen as heteroelements in the ring structure that are responsible for bonding with the metal surface and help to reduce corrosion of metals such as mild steel (MS) in an acidic medium. In this study, the alkaloid of Rhynchostylis retusa (RR) was extracted by solvent extraction method and confirmed by chemical test as well as FTIR spectroscopic test. Extracted alkaloids were tested as green inhibitors for the MS corrosion in a 1.0 M H2SO4 solution. The inhibition efficiency (IE) of alkaloid extracts of RR was studied by the weight loss measurement method and electrochemical polarization method. Results showed that the maximum IE in the gravimetric method was 87.51% in 1000 ppm solution at 6 h immersion time. Open circuit potential (OCP) and potentiodynamic polarization results indicated that the extracted alkaloids acted as a mixed type of inhibitor. IE by polarization method was found to be 93.24% for the sample immersed for 6 h. The temperature effect study reveals that inhibitors can work only below 35 °C. Alkaloids of RR can be successfully extracted and used as corrosion inhibitors for MS in an acidic medium below 35 °C

Trace level monitoring of Cu(II) ion using CuS particles based membrane electrochemical sensor

Cu(II) monitoring is a matter of great interest to researchers due to its toxicity and adverse environmental effects. Among different methods for detecting Cu(II), ion-selective electrode (ISE) is more advantageous as they are low-cost, easy to fabricate, and highly selective. Here, we report a simple, inexpensive, and reproducible procedure for the fabrication of Cu(II) ion-selective electrodes using CuS particles and polyvinyl chloride (PVC) as a matrix. CuS particles, obtained by chemical precipitation, were characterized using X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, ultraviolet-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), and energy-dispersive X-Ray spectroscopy (EDX). Optimization of the membrane compositions was done to get a well-behaved sensor by varying amounts of CuS, PVC, and acetophenone (AP). A membrane composition of 0.4 g CuS, 0.5 g PVC, and 1.0 mL AP in 5.0 mL tetrahydrofuran (THF) gave a Nernstian slope of 27.31 mV per decade change of Cu(II) ion over a wide range of concentration down to 64 ppb (1 × 10−6 M). The sensor gave a fast response time of 25 s, and it indicated the endpoint in a potentiometric titration of Cu(II) with standard EDTA solution. A pH-independent potential response was obtained in the pH 4.0–6.0

Abstracts of AICTE Sponsored International Conference on Post-COVID Symptoms and Complications in Health

This book presents the selected abstracts of the International Conference on Post-COVID Symptoms and Complications in Health, hosted from the 28th to 29th of April 2022 in virtual mode by the LR Institute of Pharmacy, Solan (H.P.)-173223 in Collaboration with AICTE, New Delhi. This conference focuses on the implications of long-term symptoms on public health, ways to mitigate these complications, improve understanding of the disease process in COVID-19 patients, use of computational methods and artificial intelligence in predicting complications, and the role of various drug delivery systems in combating the complications. Conference Title:  International Conference on Post-COVID Symptoms and Complications in HealthConference Sponsor: AICTE, New Delhi.Conference Date: 28-29 April 2022Conference Location: OnlineConference Organizer: LR Institute of Pharmacy, Solan (H.P.)-173223

Abstracts of AICTE Sponsored International Conference on Post-COVID Symptoms and Complications in Health

This book presents the selected abstracts of the International Conference on Post-COVID Symptoms and Complications in Health, hosted from the 28th to 29th of April 2022 in virtual mode by the LR Institute of Pharmacy, Solan (H.P.)-173223 in Collaboration with AICTE, New Delhi. This conference focuses on the implications of long-term symptoms on public health, ways to mitigate these complications, improve understanding of the disease process in COVID-19 patients, use of computational methods and artificial intelligence in predicting complications, and the role of various drug delivery systems in combating the complications. Conference Title:  International Conference on Post-COVID Symptoms and Complications in HealthConference Sponsor: AICTE, New Delhi.Conference Date: 28-29 April 2022Conference Location: OnlineConference Organizer: LR Institute of Pharmacy, Solan (H.P.)-173223