The antinociceptive effects of rubbing Pistacia atlantica oil by formalin test in male labarotory mice

Background and Aim: Baneh (Pistacia Atlantica) is a valuable oily fruit that has gained considerable attention because of its pharmaceutical, nutritional and industrial applications. This study aimed to investigate the antinociceptive effect of rubbing baneh oil using formalin test during acute and chronic phases in male laboratory mice. Materials and Methods: This experimental study was conducted on 30 male mice. They were allocated into 5 groups including control (without treatment), rubbing vaseline, morphine (positive control), baneh oil, and baneh oil plus morphine. Before the formalin test, the animals were treated with rubbing baneh oil for six hours. Morphine (10 mg/kg) was injected with a single dose subcutaneously. Pain scores were obtained using formalin test by injecting 0.02 ml of 2.5% formalin into the animals&rsquo; right palm. Results: Rubbing baneh oil significantly reduced the pain in acute pain (P <0.01), sub-acute pain (P <0.001), and chronic pain (P <0.001) phases as compared with the control group. On the other hand, baneh oil showed a stronger antinociceptive effect in chronic pain than morphine (P <0.01). Conclusion: This study showed that rubbing Baneh oil has antinociceptive effect where a considerable share of this effect may be attributed to the oil&rsquo;s compounds. &nbsp

The Analgesic Effect of Topical Clove Oil Using Formalin Test in Male Mice

Background & objectives: Syzygium aromaticum (Clove) is a medicinal plant usually used in traditional medicine to reduce toothache. The aim of the present study was to evaluate the analgesic effect of topical Clove oil on acute and chronic pain in male mice using formalin test. Methods: In this study, 24 mice were divided into 4 groups: control, morphine (as positive control), Clove oil, and Clove oil plus morphine groups. Before the formalin test, the animals were treated topically with clove oil for 6 hours.&nbsp; A single dose of morphine (10 mg/kg) was prescribed subcutaneously. Pain scores were obtained using the formalin test through an injection of 20 microliter of formalin 2.5% into the palm of the animal's right hand. Results: Topical administration of Clove oil significantly decreased acute pain (the initial phase of the formalin test) and chronic pain (the second phase of the formalin test). Furthermore, topical Clove oil increased the analgesic effect of morphine in acute pain phase. Conclusions: This study showed that the analgesic effect of topical Clove oil was comparable to morphine