Nucleotide sequence and annealing temperatures of primers generating polymorphic ISSR products in the analysed genotypes.

<p>*R purine, Y pyrimidine; N any nucleotide; B indicates C, G or T; D as A, G, or T; H as A, C, or T; and V as A, C, or G.</p

Mean squares from variance analysis for 1000-seed weight and seed coat percentage.

<p>*significance at the level of α = 0.05.</p><p>**significance at the level of α = 0.01.</p

Weight of 1000 seed and seed coat percentage in analysed genotypes of <i>Lupinus angustifolius</i> L.

<p>Means for each year based of three replications ± standard errors.</p><p>A,B,C …-uniform groups based on the Duncan test.</p><p>AU-originated from Australia.</p><p>PL-originated from Poland.</p><p>BY - originated from Belarus.</p><p>SEM – standard error of the means.</p

The linear regression between weight of 1000 seeds and seed coat for analysed genotypes <i>Lupinus angustifolius</i>.

<p>The linear regression between weight of 1000 seeds and seed coat for analysed genotypes <i>Lupinus angustifolius</i>.</p

<i>Lupinus angustifolius</i> seed sculpture in the mutant lines PL-41H (A, A′), PL-44H (B, B′), PL-53H (C, C′), PL-‘Emir’ (D, D′), PL-‘Sonet’ (E, E′).

<p>SEM images of seed surface (A′, B′, C′, D′, E′ – general view of seeds in inserts) and cross-section of the seed coat (A, B, C, D, E). C – cuticle; MS – macrosclereids layer; OS – osteosclereids layer; PA – parenchymal cells.</p

Products of PCR-ISSR amplification after using of UBC 815 for analysed genotypes <i>Lupinus angustifolius</i>: 1 – AU-11257-19/1, 2 – PL-Sonet, 3 – PL-53H, 4 – AU-Walup, 5 – PL-44H, 6 – AU-Yorrel, 7 – PL-41H, 8 – PL-Zeus, 9 – BY-LAG24, 10 – PL-Emir.

<p>Products of PCR-ISSR amplification after using of UBC 815 for analysed genotypes <i>Lupinus angustifolius</i>: 1 – AU-11257-19/1, 2 – PL-Sonet, 3 – PL-53H, 4 – AU-Walup, 5 – PL-44H, 6 – AU-Yorrel, 7 – PL-41H, 8 – PL-Zeus, 9 – BY-LAG24, 10 – PL-Emir.</p

<i>Lupinus angustifolius</i> seed sculpture in PL-Zeus (A, A′), BY-LAG24 (B, B′), AU-11267-19 (C, C′), AU-Walup (D, D′), AU-Yorrel (E, E′).

<p>SEM images of the seed surface (A′, B′, C′, D′ E′, – general view of seeds in inserts) and cross-section of the seed coat (A, B, C, D, E). C – cuticle; MS – macrosclereids layer; OS – osteosclereids layer; PA – parenchymal cells.</p

Genetic identity (Is - below the diagram) and genetic distance (Ds - above the diagonal) statistics for the analysed genotypes of <i>L. angustifolius</i>.

<p>Genetic identity (Is - below the diagram) and genetic distance (Ds - above the diagonal) statistics for the analysed genotypes of <i>L. angustifolius</i>.</p