Involvement of TIP60 acetyltransferase in intracellular Salmonella replication

<p>Abstract</p> <p>Background</p> <p><it>Salmonella </it>enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed <it>Salmonella </it>containing vacuole (SCV). The biogenesis of SCV requires <it>Salmonella </it>type III protein secretion/translocation system and their effector proteins which are translocated into host cells to exploit the vesicle trafficking pathways. SseF is one of these effectors required for SCV formation and Intracellular <it>Salmonella </it>replication through unknown mechanisms.</p> <p>Results</p> <p>In an attempt to identify host proteins that interact with SseF, we conduct a yeast two-hybrid screening of human cell cDNA library using SseF as the bait. We identified that TIP60, an acetyltransferase, interacts with SseF. We showed that the TIP60 acetylation activity was increased in the presence of SseF, and TIP60 was upregulated upon <it>Salmonella </it>infection. In addition, TIP60 is required for efficient intracellular <it>Salmonella </it>replication in macrophages.</p> <p>Conclusion</p> <p>Taken together, our data suggest that <it>Salmonella </it>may use SseF to exploit the host TIP60 acetyltransferase activity to promote efficient <it>Salmonella </it>replication inside host cells.</p

Optimal Design and Simulation for the Intelligent Control of Sewage Treatment Based on Multi-Objective Particle Swarm Optimization

With the continuous increase in emphasis on the environmental protection industry, sewage treatment plants have been built in many places, and these sewage treatment plants undoubtedly occupy an important position in protecting the local environment. The sewage treatment process is generally complicated and the treatment environment is difficult, which means that the treatment plant must have an excellent control system. At this stage, the sewage treatment systems in many cities have the issue of possessing backward technology and huge costs, which hinder the development of urban sewage treatment. In this paper, a new intelligent control method for sewage treatment is proposed, combined with the multi-objective particle swarm optimization (MOPSO) algorithm. The MOPSO algorithm is used to optimize the parameters and control rules of the controller globally, thereby improving the performance and work efficiency of the controller. Practice has shown that the intelligent control system combined with the MOPSO algorithm can make chemical oxygen demand (COD) in the sewage treatment quickly meet the expected requirements, and the control accuracy is also very accurate, which greatly improves the sewage treatment performance. Through our calculations, the new method improved the sewage treatment efficiency by 7.15%

Global classical solutions to the viscous two-phase flow model with slip boundary conditions in 3D exterior domains

Abstract We consider the two-phase flow model in 3D exterior domains with slip boundary conditions. We establish the global existence of classical solutions of this system, provided that the initial energy is suitably small. Furthermore, we prove that the pressure has large oscillations and contains vacuum states when the initial pressure allows large oscillations and a vacuum. Finally, we also obtain the large-time behavior of the classical solutions

Influence of Pressure on the Mechanical and Electronic Properties of Wurtzite and Zinc-Blende GaN Crystals

The mechanical and electronic properties of two GaN crystals, wurtzite and zinc-blende GaN, under various hydrostatic pressures were investigated using first principles calculations. The results show that the lattice constants of the two GaN crystals calculated in this study are close to previous experimental results, and the two GaN crystals are stable under hydrostatic pressures up to 40 GPa. The pressure presents extremely similar trend effect on the volumes of unit cells and average Ga-N bond lengths of the two GaN crystals. The bulk modulus increases while the shear modulus decreases with the increase in pressure, resulting in the significant increase of the ratios of bulk moduli to shear moduli for the two GaN polycrystals. Different with the monotonic changes of bulk and shear moduli, the elastic moduli of the two GaN polycrystals may increase at first and then decrease with increasing pressure. The two GaN crystals are brittle materials at zero pressure, while they may exhibit ductile behaviour under high pressures. Moreover, the increase in pressure raises the elastic anisotropy of GaN crystals, and the anisotropy factors of the two GaN single crystals are quite different. Different with the obvious directional dependences of elastic modulus, shear modulus and Poisson&#8217;s ratio of the two GaN single crystals, there is no anisotropy for bulk modulus, especially for that of zinc-blende GaN. Furthermore, the band gaps of GaN crystals increase with increasing pressure, and zinc-blende GaN has a larger pressure coefficient. To further understand the pressure effect on the band gap, the band structure and density of states (DOSs) of GaN crystals were also analysed in this study

Influence of Pressure on the Mechanical and Electronic Properties of Wurtzite and Zinc-Blende GaN Crystals

The mechanical and electronic properties of two GaN crystals, wurtzite and zinc-blende GaN, under various hydrostatic pressures were investigated using first principles calculations. The results show that the lattice constants of the two GaN crystals calculated in this study are close to previous experimental results, and the two GaN crystals are stable under hydrostatic pressures up to 40 GPa. The pressure presents extremely similar trend effect on the volumes of unit cells and average Ga-N bond lengths of the two GaN crystals. The bulk modulus increases while the shear modulus decreases with the increase in pressure, resulting in the significant increase of the ratios of bulk moduli to shear moduli for the two GaN polycrystals. Different with the monotonic changes of bulk and shear moduli, the elastic moduli of the two GaN polycrystals may increase at first and then decrease with increasing pressure. The two GaN crystals are brittle materials at zero pressure, while they may exhibit ductile behaviour under high pressures. Moreover, the increase in pressure raises the elastic anisotropy of GaN crystals, and the anisotropy factors of the two GaN single crystals are quite different. Different with the obvious directional dependences of elastic modulus, shear modulus and Poisson’s ratio of the two GaN single crystals, there is no anisotropy for bulk modulus, especially for that of zinc-blende GaN. Furthermore, the band gaps of GaN crystals increase with increasing pressure, and zinc-blende GaN has a larger pressure coefficient. To further understand the pressure effect on the band gap, the band structure and density of states (DOSs) of GaN crystals were also analysed in this study.</p

<i>Solanum lycopersicum</i>, a Model Plant for the Studies in Developmental Biology, Stress Biology and Food Science

Fruits, vegetables and other plant-derived foods contribute important ingredients for human diets, and are thus favored by consumers worldwide. Among these horticultural crops, tomato belongs to the Solanaceae family, ranks only secondary to potato (S. tuberosum L.) in yields and is widely cultivated for fresh fruit and processed foods owing to its abundant nutritional constituents (including vitamins, dietary fibers, antioxidants and pigments). Aside from its important economic and nutritional values, tomato is also well received as a model species for the studies on many fundamental biological events, including regulations on flowering, shoot apical meristem maintenance, fruit ripening, as well as responses to abiotic and biotic stresses (such as light, salinity, temperature and various pathogens). Moreover, tomato also provides abundant health-promoting secondary metabolites (flavonoids, phenolics, alkaloids, etc.), making it an excellent source and experimental system for investigating nutrient biosynthesis and availability in food science. Here, we summarize some latest results on these aspects, which may provide some references for further investigations on developmental biology, stress signaling and food science

Inactivation of Escherichia coli and Staphylococcus aureus by using a UVC-LED module with a multi-wavelength setting

UVC-LED is known as a deep ultraviolet LED. The application development and disinfection efficiency of UVC-LED modules are important problems encountered when UVC-LED products are rushed into commercialization. In this article, a specific disinfection experiment with a UVC-LED module was combined to analyze the disinfection efficiency. UVC-LEDs with wavelengths of 260 and 280 nm were used and supplemented with UVA-LEDs with wavelengths of 360 and 390 nm. The module was packaged to investigate the inactivation of Escherichia coli and Staphylococcus aureus. Two new findings were obtained through the analysis and comparison of the experiments. First, the short wavelength from UVA might have an enhanced destructive effect on microorganisms when the radiation intensity of UVA-LED was sufficient with coupling UVA and UVC. Second, 260 nm UVC-LED lamp beads might have a shorter response time to inactivate microorganisms than 280 nm UVC-LED lamp beads. Bactericidal experiments near the surface and different radiation distances showed that the inactivation rate reached 99.9% after 1 min of exposure when the UVC-LED module was set at 260 or 280 nm wavelength lamp beads for disinfection. The disinfection efficiency of 280 nm UVC-LED lamp beads was higher than that of 260 nm UVC-LED lamp beads because of the increased UV intensity. The radiation distance was within 7.5 cm range, the exposure time was 60 s, the inactivation rate was over 99.9%, and the disinfection effect was remarkable. For current UVC-LED applications, such as near-surface UVC-LED, disinfection and air purification products have a high value.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Components, Technology and Material