Demonstrating the antinociceptive action of renin-angiotensin system modulators in mice

Research done in the present study belongs to a wider area of experimental determinations on nociception models, when using laboratory animals. Their aim is to determine the ED50 value (Efficient dose 50) in conditions of inflammatory (chemical stimulus) and non-inflammatory (thermic stimulus) antinociception of some renin-angiotensin system modulators: captopril, ramipril, candesartan. The experimental determinations were realized accordingly to bioethical regulations concerning laboratory animals. The study has used Swiss mice, weighing between 20-30g, being held in constant temperature (21°C ± 2°C) and a dark / light cycle of 12 hours (7.00 AM / 7.00 PM). The researched substances are administered as CMC-Na 0.1% suspensions in geometrical progression doses. The following nociception models have been used: abdominal constrictive response test, hot plate test, formalin test. The abdominal constrictive response test has been evaluated as quantal, the hot plate test and the formalin test have been interpreted as gradual. The regression line, the correlation coefficient and the interval of trust for each substance and studied model have been analyzed. The obtained ED50 values are compared to each other to evaluate the potency of the substances for each nociception model. The obtained data is used for realizing fixed-ratio antinociceptive combinations

Camelina sativa Methanolic and Ethanolic Extract Potential in Alleviating Oxidative Stress, Memory Deficits, and Affective Impairments in Stress Exposure-Based Irritable Bowel Syndrome Mouse Models

Camelina sativa is mainly used as an oilseed crop; its edible oil is being also used as a traditional home remedy for the treatment of ulcers, wounds, and eye inflammations, due to the antioxidant activities. In the present study, the chemically characterized alcoholic extracts of Camelina sativa var. Madalina defatted seeds (5 g/kg body weight p.o., suspended in CMC-Na 0.1%) were administered to stress-induced animal models of irritable bowel syndrome (based on combinations of contention stress and multifactorial stress and maternal stress) and evaluated for the behavioural (short-term memory by the Y maze test, the anxious behaviour using the elevated plus maze test, and the antidepressant effect using the forced swimming test) and brain and bowel tissue oxidative status (superoxide dismutase and glutathione peroxidase enzymes activities and malondialdehyde and total soluble protein levels) improving effects. According to the chemical characterization, the extracts were rich in sinapine, glucosinolates, and flavonol glycosides. Moreover, this study showed the beneficial effects of Camelina sativa seed methanolic and ethanolic extracts on the behaviour and brain and bowel tissues oxidative stress status of stress exposure-based IBS mouse models. Despite the slight differences in the chemical composition of the methanolic and ethanolic extracts, the results suggested that the Camelina sativa extracts could reverse the short-term memory impairments caused by stress exposure and also could decrease the intensity and frequency of the anxiety and depressive-like behaviours observed in the stress-exposed animal models of IBS. Furthermore, the Camelina sativa extracts showed a significant effect on the oxidative stress markers in the brain and bowel tissues of the studied animal model by decreasing the superoxide dismutase activity and increasing the glutathione peroxidase activity. However, the results suggested that the extracts could also increase lipid peroxidation in bowel tissues. In this way, this study provides additional evidence that the administration of Camelina sativa seed alcoholic extracts could improve cognitive performances and mood and exhibit the antioxidant capacity in both the brain and bowel tissues