The Parent Populations of 6 groups identified from Chemical Tagging in the Solar neighborhood

We estimate the size and distribution of the parent populations for the 6 largest (at least 20 stars in the Solar neighborhood) chemical groups identified in the Chemical Tagging experiment by Mitschang et al.~2014. Stars in the abundance groups tend to lie near a boundary in angular momentum versus eccentricity space where the probability is highest for a star to be found in the Solar neighborhood and where orbits have apocenter approximately equal to the Sun's galactocentric radius. Assuming that the parent populations are uniformly distributed at all azimuthal angles in the Galaxy, we estimate that the parent populations of these abundance groups contain at least 200,000 members. The spread in angular momentum of the groups implies that the assumption of a uniform azimuthal distribution only fails for the two youngest groups and only for the highest angular momentum stars in them. The parent populations of three thin disk groups have narrow angular momentum distributions, but tails in the eccentricity and angular momentum distributions suggest that only a small fraction of stars have migrated and increased in eccentricity. In contrast, the parent populations of the thick disk groups exhibit both wide angular momentum and eccentricity distributions implying that both heating and radial migration has taken place.Comment: accepted for publication in MNRA

Postsynaptic Elevation of Calcium Induces Persistent Depression of Developing Neuromuscular Synapses

AbstractSynaptic activity is known to modulate neuronal connectivity in the nervous system. At developing Xenopus neuromuscular synapses in culture, repetitive postsynaptic application of ACh near the synapse leads to immediate and persistent synaptic depression, which was shown to be caused by reduction of presyaptic evoked transmitter release. However, little depression was found when ACh was applied to the muscle 20 μm or further from the synapse. Fluorescence imaging of cytosolic Ca2+ ([Ca2+]i) showed that each ACh pulse induced a transient elevation of myocyte [Ca2+]i that spread approximately 20 μm. Local photoactivated release of Ca2+ from the caged Ca2+ chelators nitr-5 or nitrophen in the postsynaptic cell was sufficient to induce persistent synaptic depression. These results support a model in which localized Ca2+ influx into the postsynaptic myocyte initiates transsynaptic retrograde modulation of presynaptic secretion mechanisms

What is a galaxy? How Cold is Cold Dark Matter? Recent progress in Near Field Cosmology

There has been a vast recent improvement in photometric and kinematic data for star clusters, Ultra Compact dwarfs, galactic nuclei, and local dSph galaxies, with Subaru contributing substantially to the photometric studies in particular. These data show that there is a bimodal distribution in half-light radii, with stable star clusters always being smaller than 35pc, while stable galaxies are always larger than 120pc. We extend the previously known observational relationships and interpret them in terms of a more fundamental pair of intrinsic properties of dark matter itself: dark matter forms cored mass distributions, with a core scale length of greater than about 100pc, and always has a maximum central mass density with a narrow range. The dark matter in dSph galaxies appears to be clustered such that there is a mean volume mass density within the stellar distribution which has the very low value of about 0.1$M_{\odot}$ pc$^{-3}$. None of the dSphs displays kinematics which require the presence of an inner cusp, while in two dSphs there is evidence that the density profile is shallow (cored) in the inner regions. The maximum central dark matter density derived is model dependent, but is likely to have a mean value (averaged over a volume of radius 10pc) of about 0.1$M_{\odot}$ pc$^{-3}$, which is 5GeV/c$^2$cm$^{-3}$). Galaxies are embedded in dark matter halos with these properties; smaller systems containing dark matter are not observed.Comment: proceedings of The 1st Subaru International Conference, "Panoramic Views of Galaxy Formation and Evolution" (Hayama, Japan, 11-16 Dec 2007) ASP Conf se

A New Giant Stellar Structure in the Outer Halo of M31

The Sloan Digital Sky Survey has revealed an overdensity of luminous red giant stars ~ 3 degrees (40 projected kpc) to the northeast of M31, which we have called Andromeda NE. The line-of-sight distance to Andromeda NE is within approximately 50 kpc of M31; Andromeda NE is not a physically unrelated projection. Andromeda NE has a g-band absolute magnitude of ~ -11.6 and central surface brightness of ~ 29 mag/sq.arcsec, making it nearly two orders of magnitude more diffuse than any known Local Group dwarf galaxy at that luminosity. Based on its distance and morphology, Andromeda NE is likely undergoing tidal disruption. Andromeda NE's red giant branch color is unlike that of M31's present-day outer disk or the stellar stream reported by Ibata et al. (2001), arguing against a direct link between Andromeda NE and these structures. However, Andromeda NE has a red giant branch color similar to that of the G1 clump; it is possible that these structures are both material torn off of M31's disk in the distant past, or that these are both part of one ancient stellar stream.Comment: 11 pages, 3 figures; ApJ Letters accepted versio

The Shortest Period Detached Binary White Dwarf System

We identify SDSS J010657.39-100003.3 (hereafter J0106-1000) as the shortest period detached binary white dwarf (WD) system currently known. We targeted J0106-1000 as part of our radial velocity program to search for companions around known extremely low-mass (ELM, ~ 0.2 Msol) WDs using the 6.5m MMT. We detect peak-to-peak radial velocity variations of 740 km/s with an orbital period of 39.1 min. The mass function and optical photometry rule out a main-sequence star companion. Follow-up high-speed photometric observations obtained at the McDonald 2.1m telescope reveal ellipsoidal variations from the distorted primary but no eclipses. This is the first example of a tidally distorted WD. Modeling the lightcurve, we constrain the inclination angle of the system to be 67 +- 13 deg. J0106-1000 contains a pair of WDs (0.17 Msol primary + 0.43 Msol invisible secondary) at a separation of 0.32 Rsol. The two WDs will merge in 37 Myr and most likely form a core He-burning single subdwarf star. J0106-1000 is the shortest timescale merger system currently known. The gravitational wave strain from J0106-1000 is at the detection limit of the Laser Interferometer Space Antenna (LISA). However, accurate ephemeris and orbital period measurements may enable LISA to detect J0106-1000 above the Galactic background noise.Comment: MNRAS Letters, in pres

Mpox in people with past infection or a complete vaccination course : a global case series

We thank the individuals with mpox who agreed to allow their medical team to share their data; the medical staff who looked after the participants; and all the listed contributors for their efforts in collecting the data.Background: Since May, 2022, a large global outbreak of human mpox (formerly known as monkeypox) has predominantly affected men who have sex with men. The strain responsible, Clade IIb, has mutated substantially from precursors originating from the 2017-18 outbreak in Nigeria. Immunity to smallpox, another orthopoxvirus, via previous infection or vaccination provides lifelong immunity. However, since the 2022 mpox outbreak, recent clusters were described in individuals with presumed immunity through recent infection or vaccination. We aim to describe the epidemiological and clinical characteristics of mpox in individuals with past infection or vaccination to improve the understanding of this disease in the setting of previous immunity. Methods: In this global case series, international collaborators from nine countries provided data on individuals with PCR-confirmed mpox after documented previous infection or vaccination between May 11, 2022, and June 30, 2023. We excluded cases that could not confirm vaccination status or cases with partial immunisation or any doses received before the current multi-national mpox outbreak (cutoff date May 1, 2022). Data were collected via a case report spreadsheet that reported on dates of infection and vaccination, route of immunisation, demographic characteristics, clinical findings, HIV status, concomitant sexually transmitted infections, and markers of disease severity (mpox severity score system). We describe case epidemiology, clinical course, and mpox severity scores; all analyses were descriptive. Findings: We report mpox infections in 37 gay and bisexual men who have sex with men: seven individuals had mpox reinfections, 29 individuals had mpox infections that occurred after two appropriately spaced Modified Vaccinia Ankara-Bavarian Nordic vaccine courses, and one individual had an infection that met the criteria for both reinfection and infection after vaccination. The median age of individuals was 36 years (IQR 30-45; range 21-58). Those with natural immunity after initial infection had a shorter disease course with less mucosal disease upon reinfection than with their initial infection. Infections post-vaccination were characterised by few lesions, little mucosal disease, and minimal analgesia requirements; two people received oral tecovirimat. Overall, there were no deaths, no bacterial superinfections, and all individuals were managed in the ambulatory clinic with one hospital admission for a necrotising neck lesion. Interpretation: The epidemiology of people with mpox reinfection or infection post-vaccination was similar to other published cohorts during the 2022 outbreak-predominantly young, sexually active gay and bisexual men who have sex with men. Clinical features and outcomes of repeat infection and infection after vaccination appear to be less clinically severe than those described in 2022 case literature. Specifically, compared with the 2022 case series, these individuals in the present study had fewer confluent lesions, less mucosal involvement, reduced analgesia requirement, and fewer admissions. Natural immunity and vaccine-induced immunity are not fully protective against mpox infection. However, in this small series both disease duration and severity appear to be reduced

Quantal Glutamate Release Is Essential for Reliable Neuronal Encodings in Cerebral Networks

Background: The neurons and synapses work coordinately to program the brain codes of controlling cognition and behaviors. Spike patterns at the presynaptic neurons regulate synaptic transmission. The quantitative regulations of synapse dynamics in spike encoding at the postsynaptic neurons remain unclear. Methodology/Principal Findings: With dual whole-cell recordings at synapse-paired cells in mouse cortical slices, we have investigated the regulation of synapse dynamics to neuronal spike encoding at cerebral circuits assembled by pyramidal neurons and GABAergic ones. Our studies at unitary synapses show that postsynaptic responses are constant over time, such as glutamate receptor-channel currents at GABAergic neurons and glutamate transport currents at astrocytes, indicating quantal glutamate release. In terms of its physiological impact, our results demonstrate that the signals integrated from quantal glutamatergic synapses drive spike encoding at GABAergic neurons reliably, which in turn precisely set spike encoding at pyramidal neurons through feedback inhibition. Conclusion/Significance: Our studies provide the evidences for the quantal glutamate release to drive the spike encodings precisely in cortical circuits, which may be essential for programming the reliable codes in the brain to manage wellorganize

Rapid Sampling of Molecular Motions with Prior Information Constraints

Proteins are active, flexible machines that perform a range of different functions. Innovative experimental approaches may now provide limited partial information about conformational changes along motion pathways of proteins. There is therefore a need for computational approaches that can efficiently incorporate prior information into motion prediction schemes. In this paper, we present PathRover, a general setup designed for the integration of prior information into the motion planning algorithm of rapidly exploring random trees (RRT). Each suggested motion pathway comprises a sequence of low-energy clash-free conformations that satisfy an arbitrary number of prior information constraints. These constraints can be derived from experimental data or from expert intuition about the motion. The incorporation of prior information is very straightforward and significantly narrows down the vast search in the typically high-dimensional conformational space, leading to dramatic reduction in running time. To allow the use of state-of-the-art energy functions and conformational sampling, we have integrated this framework into Rosetta, an accurate protocol for diverse types of structural modeling. The suggested framework can serve as an effective complementary tool for molecular dynamics, Normal Mode Analysis, and other prevalent techniques for predicting motion in proteins. We applied our framework to three different model systems. We show that a limited set of experimentally motivated constraints may effectively bias the simulations toward diverse predicates in an outright fashion, from distance constraints to enforcement of loop closure. In particular, our analysis sheds light on mechanisms of protein domain swapping and on the role of different residues in the motion

A tale of two stories: astrocyte regulation of synaptic depression and facilitation

Short-term presynaptic plasticity designates variations of the amplitude of synaptic information transfer whereby the amount of neurotransmitter released upon presynaptic stimulation changes over seconds as a function of the neuronal firing activity. While a consensus has emerged that changes of the synapse strength are crucial to neuronal computations, their modes of expression in vivo remain unclear. Recent experimental studies have reported that glial cells, particularly astrocytes in the hippocampus, are able to modulate short-term plasticity but the underlying mechanism is poorly understood. Here, we investigate the characteristics of short-term plasticity modulation by astrocytes using a biophysically realistic computational model. Mean-field analysis of the model unravels that astrocytes may mediate counterintuitive effects. Depending on the expressed presynaptic signaling pathways, astrocytes may globally inhibit or potentiate the synapse: the amount of released neurotransmitter in the presence of the astrocyte is transiently smaller or larger than in its absence. But this global effect usually coexists with the opposite local effect on paired pulses: with release-decreasing astrocytes most paired pulses become facilitated, while paired-pulse depression becomes prominent under release-increasing astrocytes. Moreover, we show that the frequency of astrocytic intracellular Ca2+ oscillations controls the effects of the astrocyte on short-term synaptic plasticity. Our model explains several experimental observations yet unsolved, and uncovers astrocytic gliotransmission as a possible transient switch between short-term paired-pulse depression and facilitation. This possibility has deep implications on the processing of neuronal spikes and resulting information transfer at synapses.Comment: 93 pages, manuscript+supplementary text, 10 main figures, 11 supplementary figures, 1 tabl