The Effect of Panzeria Alaschanica Kupr. on Carrageenan-Induced Acute Inflammation in Rats

Objectives: In the present study, we aimed to investigate the effect of Panzeria alaschanica Kupr. (P. alaschanica) on carrageenan-induced acute inflammation in rats. Method: Carrageenan-induced rat paw edema was used to evaluate the anti-inflammatory activity. P. alaschanica extract (32, 64, and 160 mg/kg/ BW) or vehicle was given orally 60 minutes before the subplantar injection of carrageenan. Ibuprofen (100 mg/kg) was used as a standard drug. The carrageenan-injected paw was measured 30, 60, 120, 180, and 240 minutes after the carrageenan injection. The levels of serum tumor necrosis factor (TNF)-α, interleukin (IL)-ELISA measured 1β, and IL-6. Results: The P. alaschanica at all given doses significantly (p˂0.01) inhibited carrageenan-induced rat paw edema. Moreover, it significantly reduced TNF-α, IL-1β, and IL-6 serum levels at different doses (p˂0.01). The anti-inflammatory effect of P. alaschanica was comparable to ibuprofen. Conclusion: P. alaschanica has an anti-inflammatory impact on carrageenan-induced paw edema in rats. The mechanism of action may partly be via reducing the levels of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6

Using Network Pharmacology to Clarify the Therapeutic Mechanism of Hataagqi-19 Hot Compress Therapy in Rats with Lumbar Disc Herniation

Objective: This study aims to determine the primary efficacy of traditional medicine treatment of Lumbar disc herniation (LDH) and the effect of Hataagqi-19 hot compress (H19+HC) therapy on LDH rats. Methods: Referencing ancient books of Mongolian medicine, we studied the theoretical basis of Mongolian medicine to treat LDH. We performed operations to establish a model of SD rats with LDH treated with H19+HC. Paw withdrawal threshold (PWT) was detected, serum TNF-α, IL-6, and IL-1β were measured by ELISA, and immunohistochemical staining was used to measure IL-6, CD68, and IgG. Results: The medicinal properties of the drugs for the treatment of LDH are mainly dry “Xieriwusu,” heat-clearing, and suppressing “Heyi” medicine. The PWT value, serum TNF-α, IL-6, IL-1β and tissue IL-6, CD68, IgG of H19+HC was lower than that of the control group. The nucleus pulposus of the H19+HC was significantly smaller than that of the control group. The positive staining result in the main inflammatory cells of the lesion was CD68. Conclusions: Mongolian medicine treatment of LDH is based on the dry “Xieriwusu,” heat-clearing, and suppressing “Heyi” efficacy. The H19+HC therapy follows this efficacy and has the effects of improved abnormal behavior, analgesic, anti-inflammatory, and reabsorption of the nucleus pulposus

The Hepatoprotective Activity of the Traditional Multicomponent Formulation Gurgem-13 on Carbon Tetrachloride (CCL4) Induced Experimental Liver Injury in Rats

Objectives: The purpose of present study was intended to evaluate hepatoprotective effects of traditional multicomponent formulation Gurgum13 on carbon tetrachloride (CCI4) induced liver injury model in rats. Methods: Chronic toxic liver injury was induced experimentally by intraperitoneal injection (IP) of 1.0 ml/kg body weight of 10% carbon tetrachloride (CCI4) three times per week for duration of three months in rats. A total of five groups of animals with 15 rats in each were used for the experiment. Group 1 was as a normal control (intact). Group 2 served as a control group received CCI4 1.0 ml/kg IP three times a week for 3 months. Group 3, 4 and 5 were as the treatment groups received CCI4 1 ml/kg IP and administered traditional multicomponent formulation Gurgum-13 as a test drug (100 mg/kg) for 3 months daily. Milk thistle (50 mg/kg) and Lilicoaguliant (LLC) (100 mg/kg) were given daily for 3 months as a reference drug respectively. The activity of Gurgum-13 formulation and other comparative drugs were investigated with the dynamic study of liver marker enzymes, AST, ALT, ALP, bilirubin, and the antioxidant and blood coagulating effects of the remedy, which were studied in three months of experimentation. Gurgum-13 is one of the Mongolian traditional multicomponent formulation used in practice for a range of diseases including liver disorders. Results: Carbon tetrachloride significantly decreased the liver functions as assessed through an increase in blood serum and liver marker enzymes in control groups. Gurgum-13 treatment significantly prevented hepatotoxicity of carbon tetrachloride by reducing ALT, AST, ALP marker enzymes and bilirubin in blood serum and liver tissue in the three months period of chronic hepatitis (p<.05) similar to reference drugs. The result of the study revealed that Gurgum-13 is significant hepatoprotective against liver injury from CCI4 induced hepatotoxicity by having antioxidant effect and decreasing lipid peroxidation in liver. Conclusion: Multicomponent traditional formulation Gurgum-13 demonstrated very good hepatoprotective effect against CCI4 induced liver injury in rats. Thus, Gurgum-13 can be an effective remedy for the treatment of chronic hepatitis and biliary tract disorders

Effects of Hepaclin-4 on Hepatocellular Carcinoma (Hep G2)

Objective: Liver cancer is the fifth most frequent cancer worldwide and is usually diagnosed at an advanced stage. Natural products play an important role in anticancer drug therapy because they are effective in treating specific characteristics of cancer while also effective at reducing side effects. However, the cytotoxicity and anti-proliferative effects of Scutellaria baicalensis Georgi, Saussurea amara L, Chiazospermum erectum (L) Bernh, Carthamus tinctorius L., remain unclear. Our research aims to investigate the anti-cancer properties of Hepaclin-4 (Hep-4) extracts from the above mentioned plants. Methods: To evaluate the in vitro cytotoxicity of Hep-4, an ethanol extract of Hep-4 (500, 250, 125, 100, 50, and 10 μg/mL)L, doxorubicin, and 5-fluorouracil (5-FU) were assessed against the Hep G2 hepatocarcinoma cell line by monitoring proliferation using the MTT assay method. Results: The results demonstrated growth inhibition of cells in both dose-and time-dependent manners. Hep-4 inhibited Hep G2 transformation in 45% and the comparative group, 5-FU, effects on 40% of it. Conclusion: Our findings suggest that Hep-4 extracts have the potential to be a potent cytotoxic agent against Hep G2 cells, as it has commendable anti-proliferative effects against hepatocarcinoma cell lines and can be considered an effective adjuvant therapeutic agent

Study on Preparation Technology and Safety Evaluation of Hataagqi-19 Hydrogel Patch

Objectives: The study objective of the present study was to screen the dosage of matrix material and clarify the safety evaluation of Hataagqi-19 hydrogel patch. Methods: Single factor and orthogonal experiments were used to screen, and the best matrix ratio was screened out with peel strength, peel ability, viscosity, spread ability, uniformity, as evaluation indicators. Long term toxicity test was carried out in SD rats in order to test general conditions such as: body weight, food intake, electrocardiogram, blood, urine, and biochemical indicators, organ coefficients and histopathological changes. Moreover, skin allergic reaction tests were done to observe the allergic responce. Results: The ratio of the gel patch matrix is NP-700: aluminum glycolate: glycerol: carbomer = 6: 0.35: 35: 0.40; the ratio of azone and propylene glycol is 1: 1, and the total penetration enhancer dosage is 5%. Hataagqi-19 preparation had no obvious long-term toxicity or skin allergies. Conclusions: The Hataagqi-19 hydrogel patch prepared with the best ratio and transdermal agent showed good performance and safety. Furthermore, we did not observed toxic side effects, and the preparation process is feasible and reliable

Relationship of Human Personal Characteristics in the three Independent Stratification Systems

Objectives: We aimed to analyze theassociation between the biophysiological characteristics of body constitution and temperament type with Mongolian Traditional Medicine (MTM) constitution type to understand further and integrate the application of these stratification systems in clinical medicine. Methods: Body constitution type was classified using the body-mass index, temperament type was determined using personality test, and dominant MTM constitution type was determined in 287 active blood donors. Age, gender, and ABO blood groups were considered as additional biophysiological characteristics. The correlation between the biophysiological characteristics and the nature of their relationship was investigated using likelihood ratio and receiver operating characteristics analysis. Results: Obesity and body-mass index were related to aging, and donor’s age and Badgan expression rate were shown as a sensitive and specific classifier for obesity state. The relationship between Badgan dominant types of human constitutions practiced in Mongolian Traditional Medicine and melancholic temperament was established. Badgan expression was a sensitive and specific classifier for melancholic and phlegmatic temperament. Conclusions: Stratification of human subjects using the primary types of human constitutions practiced in Mongolian Traditional Medicine may be helpful in clinical research and application

Blood Donors with Different Types of Human Constitution Demonstrate Different Level of Cytokines

Objective: This study was aimed to determine possible relationship between the types of human constitution used in Mongolian traditional medicine and certain types of immune response in healthy blood donors. Materials and methods: Ninety-three blood donors were enrolled in this cross-sectional study. The type of human constitution was determined using the method of Sachs R (1995) and modifi ed by Batchimeg et al. (2003), in which the titer of cytokines (Human IL-2, IL-6, IL-10, IFNγ and TGFβ) in blood plasma and in supernatant of 12- hour PBMC culture were measured by ELISA. Results: Analysis and comparison of different cytokine titers in different constitution groups produced the following fi ndings: Subjects with Badgan ( Tibetan-badken, or phlegm, or bile) type of constitution had a lower activity of Th2 mediated immune response; subjects with Shar ( Tibetan-mkrispa [kris-pa] or mucus) humour domination had a higher activity of Th2 mediated response; and subjects with Khii humour domination may demonstrate higher activity of Th1 mediated response. Conclusion: Human typology types used in Mongolian traditional medicine are likely related to activity or intensity of certain types of adaptive immune responses and authors see an essential need to continue research in this fi eld of study

Results of the Study for the Antihypertensive Effect in the Panzeria Alaschanica Kupr Plant

Objectives: To study the effect of the plant Panzeria alaschanica Kupr on lowering blood pressure. Methods: The experiment was performed using Sharon Leng Hong Ong (2009) using a male rat weighing 150 - 250 grams. On days 5, 8, 13, and 15 of the experiment, ECG, heart rate, and arterial pressure were measured in the rat’s tail with a Neurobotic systole 1.2 instruments. At the end of the experiment, the content of renin, angiotensin II and aldosterone were determined by enzyme-linked immunosorbent assay. Results: According to the results of the study, the experimental group of plant Panzeria alaschanica Kupr ingested a dose of 160 mg/kg of renin 36.8 % compared with the control group that caused the pathological model, the amount of aldosterone in animals plant Panzeria alaschanica Kupr who ingested a dose of 32 mg/kg were 43.5 % of the ingested animals compared with the pathogenic group, the amount of angiotensin II in the plant Panzeria alaschanica Kupr was 64 mg/kg animals that ingested in, was reduced by a statistically significant difference (p < 0.05) of 34.3 % compared with the control group generated by the pathological model. Conclusion: Panzeria alaschanica Kupr has a 36.8 % reduced blood pressure by affecting the renin, angiotensin, and aldosterone systems