Screening and Identification of Biosurfactant-Producing Lactic Acid Bacteria

Biosurfactant attracts people’s attention because of its advantages of green and low toxicity. Lactic acid bacteria are beneficial to human and animal health. In order to make the application of surfactants safer, SDS standard curve was established, 65 strains of Lactic acid bacteria were used as screening source, and oil expanding circle was used as index to screen the strain with strong surfactant production capacity. The results showed that the standard curve of SDS was Y=34.82+(-1495.97) X1+33.11X2, and all strains had the ability to produce surfactants. Surface activity varied with bacteria. The concentration of surface activity ranged from 111.15mg/L to 736.23 mg/L. The concentration of BS in supernatant of LB6, 49, F70, 20 and Y1 strains was selected for screening. The concentration of BS in supernatant was 561.01~935.77 mg/L, and the concentration of BS on cell surface was 401.67~1076.94 mg/L. Considering the highest BS-producing strain is F70, the result of 16SrDNA showed that the strain is Pediococcus acidilactici F70. This experiment provides basic data for the production of surfactants by Lactic acid bacteria

Effect of Amino Acids on the Production of Biosurfactant by Pediococcus Acidilactici F70

In this research, the surface activity of bacterial supernatant and cell surface was measured by the method of oil drain ring. The influence of 19 kinds of amino acids (Histidine, Threonine, Valine, Isoleucine, Leucine, Phenylalanine, Arginine, Proline, Methionine, Tryptophan, Alanine, Glycine, Glutamine, Cysteine, Hydroxyproline, Valine, Asparagine, Proline, Glutamine, Serine, and Glutamic acid, Hydroxyproline, Tyrosine) on the production of biosurfactant by Pediococcus acidilactici F70 was studied by single factor experiment, and the main amino acids promoting the production of biosurfactant were selected by Plackett–Burman design. The results showed that the yield of biological surfactant with added amino acid increased, and the yield of Glutamine produced biosurfactant in the supernatant was the highest, which was two times higher than that of the control group (414.00mg/L); the yield of biosurfactant on the cell surface was the highest when Arginine was added, which was three times higher than that of the control group. In the end, 8 of the 19 amino acids (Glycine, Tryptophan, Proline, Methionine, Arginine, Leucine, Serine and Alanine) were selected to promote the production of biosurfactants of Pediococcus acidilactici F70. The results of Plackett–Burman design showed that Alanine, Proline and Leucine had significant effects on the production of biosurfactants

Improving Fermentation of Steamed Stalk to Feed Using Candida utilis and Pachysolen tannophilus

In order to improve the protein content of straw feed and reduce the amount of nutrients added, in this paper, the cell wall structure of corn stalk was destroyed by thermophilic digestion and the polysaccharide was degraded into monosaccharide by complex enzyme, and then transformed into bacterial protein by double strain Candida utilis 1807 and Pachysolen tannophilus 1771 fermentation. Single factor experiments and orthogonal test were made to obtain the best process for the feed of double-bacteria synchronous fermentation of stalks. The optimum amount of each nutrient and the inoculation amounts of double bacteria (accounts for the percentage of the original dry straw quality): ammonium sulfate 6.79%, urea 2.72%, yeast powder 1.63%, magnesium sulfate 0.27%, Candida utilis 54.31% and Pachysolen tannophilus 54.31%; The operational parameters of fermentation process were: fermentation temperature 29°C, rotate speed 100 r/min and fermentation time 55 hours. The yield of stalk feed and crude protein was 82.04%, 23.33%, respectively. The crude protein content of stalk feed was 28.44%, which was 4.33 times of original dry stalk. The results showed that the multi-strain distribution and degradation of protein production provide important significance for corn straw bio-utilization

Effects of Nitrogen Sources and Inorganic Salts on Antioxidant Activity of Goat Milk Fermented by Lactobacillus plantarum L60

This study investigated the effects of various nitrogen sources (peptone, casein hydrolysate) and inorganic salts (KH2PO4, MgSO4 and NaCl) on the antioxidant activity (specifically, DPPH and superoxide anion scavenging rate), acidity, and pH of peptides in goat milk (GM) fermented by Lactobacillus plantarum L60 by individual factor experiments. The results indicated that nitrogen sources and inorganic salts significantly affected L. plantarum L60’s antioxidant and acid-producing abilities, and when the supplemental levels of peptone, casein hydrolysate, KH2PO4, MgSO4 and NaCl were 0.7%, 0.3%, 0.3%, 0.15% and 0.9%, respectively, the scavenging ability of antioxidant peptides on DPPH radical and superoxide anion reached the maximum

Effect of Substrate Composition on Yield and Antioxidative Activity of Exopolysaccharides From Lactobacillus fermentum B62

Exopolysaccharides (EPS) can not only give food a unique texture but also has antioxidant capacities. To select the medium composition that influences the yield and antioxidative activity of EPS, Plackett–Burman (PB) design was employed to appraise the effects of carbon sources, nitrogen sources, and inorganic salts on yield and DPPH free radical scavenging (DPPH-FRS) rate of EPS in MRS medium fermented by Lactobacillus fermentum B62. The result indicated that sucrose (p<0.01), peptone (p<0.01), and KH2PO4 (p<0.001) had the most distinguishing comprehensive effects on yield and DPPH-FRS rate of EPS, and fructose also had a noticeable effect on the two factors (p<0.05, p<0.001, respectively). Additionally, glucose (p<0.05), soy protein (p<0.001), yeast extract (p<0.01), KH2PO4 (p<0.001) and Ca(H2PO4)2 (p<0.001) significantly positive affect the yield of EPS. And inulin (p<0.05), tryptone (p<0.001), beef extract powder(p<0.001), NaH2PO4 (p<0.01) and C2H3NaO2 (p<0.05) significantly positive affect the DPPH-FRS rate of EPS. Within the test ranges, sucrose, fructose, peptone and KH2PO4 all showed significant positive relativity to the yield and anti-oxidative activity of EPS

Recent advances in emerging nanomaterials based food sample pretreatment methods for food safety screening

Food safety has been a global public health concern. Humans are seriously endangered by numerous contaminants in foodstuffs. Owning to the complexity of food matrices, sample pretreatment is an essential step both in qualitative and quantitative analysis of trace contaminants. Thus, the effective food pretreatment method is of great importance. Recently, development of nanomaterials has greatly promoted the innovation of food sample pretreatment approach. For example, carbon-based materials, metal-organic frameworks, covalent-organic frameworks, polydopamine-derived materials, silica-based materials as well as molecularly imprinted polymers showed great prospects for food sample pretreatment. In this review, we comprehensively summarized the recent pretreatment methods based on the emerging nanomaterials for food safety screening (period 2016–2019), and outlined their advantages and limitations. In the end, we also discussed the future development prospects and challenges in this field. This review hopefully can stimulate interdisciplinary research at the intersection of the emerging nanomaterials and food sample pretreatment