Development of new promising varieties of faba bean through traditional pedigree method for commercial cultivation in plain zone of India

A long term experiment was carried out in MAP Section, Department of Genetics & Plant Breeding,  CCS Haryana Agricultural University, Hisar from 2005-06 to 2018-19 on identification of elite genotypes from germplasm and their utilization in the development of high yielding variety through hybridization followed by traditional pedigree method to obtain desirable transgressive segregants in Faba bean. In the present investigation a number of germplasm lines were screened for seed yield and other related traits including national check Vikrant during 2005-06 under AICRN in augmented block design. As a result, ten elite genotypes identified i.e. EC117755, EC117799, EC248710, EC329675, HB123, HB180, HB430, HB204, HB 430, HB 502, HB 503 & one check variety, Vikrant. By using these elite genotypes, a number of F1 hybrids were made during 2005-06 and F2 to F6 generations were evaluated to identify the superior progenies during 2007-08 to 2011-12. After a long process of evaluation, rejection and selection, 20 superior transgressive segregant homozygous progenies were identified to make new entries. Later on, during 2012-2013, the superior entries (viz. HB12-1, HB12-5, HB12-8, HB12-9, HB12-11, HB12-12, HB12-13, HB12-14, HB12-15, HB12-26, HB12-28, HB12-29, HB12-30, HB12-31, HB12-34, HB12-36, HB12-37,HB12-38, HB12-39 and HB12-42) were evaluated at Hisar centre against Vikrant and found promising. Therefore, all these 20 genotypes were evaluated in SST during 2013-14, LST during 2014-15 and FYT during 2015-16. On the basis of above results, only five genotypes viz. HB12-8 (47.77q/ha), HB12-42 (47.70q/ha), HB12-15 (46.70q/ha), HB12-34 (46.14q/ha) and HB12-37 (45. 31q/ha) were found promising. These were further evaluated in multi-location trials at seven locations (i.e. Ambikpur, Delhi, Faizabad, Faridkot, Hisar, Ludhiana & Ranchi) for seed yield, quality and resistance against insect pest & disease in IVT and AVT during 2016-17 & 2017-18, respectively. Out of these, HB12-34 exhibited yield superiority over national checks,Vikrant and HFB-1 and free from insect pest and disease as well as low in vicine-covicine and high in protein content. Therefore, HB12-34 may be recommended for commercial cultivation in plain zone at national level

Monitoring of BNP cardiac biomarker with major emphasis on biosensing methods: A review

Heart failure (HF) is a prominent disease becomes a crucial cause of morbidity and mortality. It is a severe condition in which the heart is incapable to supply adequate blood to the patient's body. More than 26 million people globally exist with HF. B-type natriuretic peptide (BNP) is a reliable and promising cardiac biomarker for HF which acts as a precursor protein that passes through intracellular modification to pro-hormones. It has been recognized as a powerful biomarker among all cardiovascular diseases, specifically for HF due to its fast release kinetics and well-defined cut-off level of 100 pg/mL. Several traditional techniques have been used for the detection of BNP such as fluorescence-based assays, micro-mosaic immunoassays and immunoradiometric assays. However, these techniques are time consuming, expensive and complicated. Therefore, these limitations have been overcome by the biosensing technology that is cost effective, portable, reliable, highly sensitive and specific. Thus, biosensors have provided an effective platform for early diagnosis of BNP. Here, this review summarizes the approaches for significant analysis of BNP with special emphasis on biosensing methods and their analytical performance includes pH range, temperature, linear range and LOD score