Representative network of the genes with altered expression in MAP-challenged mice at 3 and 6 weeks p.i.

<p>Two networks showing common genes and functions at 3 and 6 weeks p.i. were merged. The purple line indicates overlapped genes and relationships between 3 and 6 weeks p.i. Individual nodes represent proteins, with relationships represented by edges. Nodes are colored to indicate changes in gene expression, with red indicating up-regulation, green indicating down-regulation, and white indicating that the gene/factor was not differentially expressed. Solid lines indicate a direct interaction and dotted lines indicate an indirect interaction. Arrows indicate directional relationships.</p

Validation of microarray data by quantitative RT-PCR.

<p>Relative expression level was normalized to the <i>GAPDH</i> expression level relative to control group by the 2^-ΔΔCT method.</p

Pathological observations.

<p><b>A)</b> histopathology scores, <b>B)</b> scores for acid-fast-positive bacilli in the liver and spleen, and <b>C)</b> representative histopathology and acid-fast stain of the spleen and liver, HE, bar = 100μm (original magnification x400; inset, x1000) Note spleen with (1); normal, (2); +2 and, (3); +4 degree of inflammation. Also note liver with (5); normal, (6); +2, and, (7); +4 degree of inflammation. Also note myriads of acid-fast positive bacilli in the liver (8) with scant numbers of bacilli in the spleen (4). Values are represented as mean ± SE, and were analyzed by Student's <i>t</i>-test for mean weight and histopathology and the Mann Whitney <i>U</i>-test for acid-fast bacilli. (***, <i>p</i> < 0.001; **, <i>p</i> < 0.01; *, <i>p</i> < 0.05).</p

Additional file 13 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 13: Supplemental Figure 8. NF2 deficiency induces anoikis resistance via YAP activation

Additional file 15 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 15: Supplemental Figure 10. Examination of lung metastasis in a subcutaneous transplantation model using Rasa1-KO S1M cells

Additional file 2 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 2: Supplemental Table 1. Correlation between RASA1 immunoreactivity and clinicopathological parame ters in human GC tissues. P-value, Chi squared test

Additional file 17 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 17: Supplemental Figure 12. Analysis of Wnt inhibitor expression in response to Nf2-KO and YAP/TAZ signaling modulation

Additional file 20 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 20: Supplemental Figure 15. Immunohistochemical analysis following YAP and Bcl-xL inhibitor treatment in Nf2/Rasa1-double KO S1M peritoneal dissemination model

Additional file 1 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 1: Materials and methods

Additional file 8 of Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency

Additional file 8: Supplemental Figure 3. In vivo validation of target genes using the spleno-hepatic metastasis model