MiR–20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells

Additional file 3: Figure S2. The protein level of PARP and caspase3 detected by western in NCM, 5PM, NCA, 5PA, si-NC and si-Rab27B transfected CNE-2 and CNE-1 cells respectively

Interkingdom multi-omics analysis reveals the effects of nitrogen application on growth and rhizosphere microbial community of Tartary buckwheat

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is an important pseudocereal crop with excellent edible, nutritional and medicinal values. However, the yield of Tartary buckwheat (TB) is very low due to old-fashioned cultivation techniques, particularly unreasonable application of nitrogen fertilizer. To improve the understanding on the theories of nitrogen use in TB, the effects of nitrogen application on growth, as well as chemical properties and microbial community of rhizosphere soil were investigated in this study. Nitrogen application could promote the plant height, stem diameter, nitrogen accumulation and yield of TB. The relative abundance and diversity of bacteria and fungi in the rhizosphere soil of TB were improved by nitrogen fertilizer. Nitrogen application increased the abundance of beneficial bacteria such as Lysobacter and Sphingomonas in rhizosphere soil, and decreased the abundance of pathogenic fungi such as Fusarium and Plectosphaerella. The results indicated that nitrogen application changed the distribution of microbial communities in TB rhizosphere soil. Furthermore, the specific enriched or depleted microorganisms in the rhizosphere soil of four TB varieties were analyzed at OTU level. 87 specific nitrogen-responsive genes with sequence variation were identified in four varieties by integrating genomic re-sequencing and transcriptome analysis, and these genes may involve in the recruitment of specific rhizosphere microorganisms in different TB varieties. This study provided new insights into the effects of nitrogen application on TB growth and rhizosphere microbial community, and improved the understanding on the mechanisms of TB root–microbe interactions

Variations in Accumulation of Lignin and Cellulose and Metabolic Changes in Seed Hull Provide Insight into Dehulling Characteristic of Tartary Buckwheat Seeds

Tartary buckwheat (Fagopyrum tataricum) is considered a profitable crop that possesses medicinal properties, because of its flavonoid compounds. However, the dehulling issue is becoming the bottleneck for consumption of Tartary buckwheat seed. In this study, we investigated the relation between dehulling efficiency and content of lignin and cellulose in the seed hull. Moreover, the untargeted metabolomics analysis, including partial least squares discriminant analysis (PLS-DA) and principal component analysis (PCA), were performed to examine the pattern of metabolic changes in the hull of Tartary buckwheat seeds, XQ 1 and MQ 1, during seed development using gas chromatography mass spectrometry (GC-MS). In mature seed hull the accumulation of highest lignin and lowest cellulose were observed in the hull of MQ 1 seed, a dehulling-friendly variety with highest dehulling efficiency (93%), than that in other dehulling recalcitrant varieties, such as XQ 1 with a range of dehulling efficiency from 2% to 6%. During seed development, the total content of lignin and cellulose increased. MQ 1 and XQ 1 displayed a similar trend in the change of lignin and cellulose that the content was decreased in lignin and increased in cellulose. PCA result showed the metabolic differentiations between MQ 1 and XQ 1 during seed development. The results of our study suggest the compensatory regulation of lignin and cellulose deposition in the hull of mature and developing seed, and deviation of MQ 1 from the ratio of lignin to cellulose of other dehulling recalcitrant varieties may have been a contributing factor that resulted in the dehulling differentia

Tousled-like kinase regulates cytokine-mediated communication between cooperating cell types during collective border cell migration.

Collective cell migration is emerging as a major contributor to normal development and disease. Collective movement of border cells in the Drosophila ovary requires cooperation between two distinct cell types: four to six migratory cells surrounding two immotile cells called polar cells. Polar cells secrete a cytokine, Unpaired (Upd), which activates JAK/STAT signaling in neighboring cells, stimulating their motility. Without Upd, migration fails, causing sterility. Ectopic Upd expression is sufficient to stimulate motility in otherwise immobile cells. Thus regulation of Upd is key. Here we report a limited RNAi screen for nuclear proteins required for border cell migration, which revealed that the gene encoding Tousled-like kinase (Tlk) is required in polar cells for Upd expression without affecting polar cell fate. In the absence of Tlk, fewer border cells are recruited and motility is impaired, similar to inhibition of JAK/STAT signaling. We further show that Tlk in polar cells is required for JAK/STAT activation in border cells. Genetic interactions further confirmed Tlk as a new regulator of Upd/JAK/STAT signaling. These findings shed light on the molecular mechanisms regulating the cooperation of motile and nonmotile cells during collective invasion, a phenomenon that may also drive metastatic cancer

The first complete chloroplast genome of Fagopyrum leptopodum (Diels) Hedberg (Caryophyllales: Polygonaceae) with phylogenetic implications

In the present study, we sequenced and assembled the complete chloroplast genome of Fagopyrum leptopodum (Diels) Hedberg. The chloroplast genome of F. leptopodum was composed of 85 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The F. leptopodum chloroplast genome is 159,375 bp in length, with a GC content of 37.81%. Phylogenetic analysis based on the combined chloroplast gene dataset indicated that the F. leptopodum exhibited a close relationship with Fagopyrum luojishanense

Analysis of coast-by noise of heavy truck tires

AbstractHeavy truck tires are one of the main sources of road traffic noise. However, the mechanism and propagation of the noise generated by these tires have not been systematically investigated. To determine the noise of heavy truck tires with different structures and patterns, and to analyze the correlation between the indoor tire noise and coast-by tire noise, an integrated tire indoor noise test and a coast-by noise test were designed and successfully implemented. The indoor test was conducted on a drum inside a semi-anechoic chamber to simultaneously measure the near field and far field noise of the tires. The outdoor measurements were carried out using a coast-by test on the new ISO 10844 surface. A formula for quantitative analysis with appropriate corrections was developed to analyze the data with reasonable errors, which can be used to predict the coast-by noise through the indoor tire noise test accurately and effectively. The analysis shows that when trying to build the relationship between indoor and outdoor heavy truck tire noise, care should be taken to differentiate the tires with a load capacity index in excess of 121 and without any dual fitting indication from ordinary tires, due to the specified test procedure

Relationship between stem characteristics and lodging resistance of Tartary buckwheat (Fagopyrum tataricum)

Stem lodging can limit the grain yield of Tartary buckwheat (Fagopyrum tataricum), and creates difficulties in harvesting. A 2-year study was conducted to study the relationships between stem characteristics and lodging resistance in Tartary buckwheat. Six Tartary buckwheat cultivars with three stem types (short-stemmed XQ1 and CQ1; mid-length stemmed YQ1 and QK3; and tall-stemmed DAB and DN1) were used. The stem characteristics differed significantly among the three stem types. Mid-length stems had the thickest stem wall, the greatest numbers of large and small vascular bundles, and the lowest stem diameter/stem wall thickness ratio among the three stem types. Mid-length stems had the highest stem breaking strength (SBS) and stem puncture strength (SPS) among the three types of stem. The lignin content was significantly higher in mid-length stems than in tall and short stems. The activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and cinnamyl alcohol dehydrogenase (CAD), but not 4-coumarate: CoA ligase (4CL), differed significantly among the three stem types. In correlation analyses, SBS was significantly positively correlated with stem diameter, stem wall thickness, and the number of large and small vascular bundles, but significantly negatively correlated with stem diameter/stem wall thickness and lodging rate. The lignin content was significantly positively correlated with the activities of lignin-related enzymes and with SBS and SPS, and significantly negatively correlated with lodging rate. The present study provides preliminary insights into the relationship between stem traits and lodging resistance in Tartary buckwheat, laying a foundation for a further understanding of lodging resistance. Abbreviations SBS: stem breaking strength; SPS: stem puncture strength; PAL: phenylalanine ammonia-lyase; TAL: tyrosine ammonia-lyase; CAD: cinnamyl alcohol dehydrogenase; 4CL: 4-coumarate: CoA ligase