DHash table

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2006.Includes bibliographical references (p. 123-132) and index.DHash is a new system that harnesses the storage and network resources of computers distributed across the Internet by providing a wide-area storage service, DHash. DHash frees applications from re-implementing mechanisms common to any system that stores data on a collection of machines: it maintains a mapping of objects to servers, replicates data for durability, and balances load across participating servers. Applications access data stored in DHash through a familiar hash-table interface: put stores data in the system under a key; get retrieves the data. DHash has proven useful to a number of application builders and has been used to build a content-distribution system [31], a Usenet replacement [115], and new Internet naming architectures [130, 129]. These applications demand low-latency, high-throughput access to durable data. Meeting this demand is challenging in the wide-area environment. The geographic distribution of nodes means that latencies between nodes are likely to be high: to provide a low-latency get operation the system must locate a nearby copy of the data without traversing high-latency links.(cont.) Also, wide-area network links are likely to be less reliable and have lower capacities than local-area network links: to provide durability efficiently the system must minimize the number of copies of data items it sends over these limited capacity links in response to node failure. This thesis describes the design and implementation of the DHash distributed hash table and presents algorithms and techniques that address these challenges. DHash provides low-latency operations by using a synthetic network coordinate system (Vivaldi) to find nearby copies of data without sending messages over high-latency links. A network transport (STP), designed for applications that contact a large number of nodes, lets DHash provide high throughput by striping a download across many servers without causing high packet loss or exhausting local resources. Sostenuto, a data maintenance algorithm, lets DHash maintain data durability while minimizing the number of copies of data that the system sends over limited-capacity links.by Frank Dabek.Ph.D

CFS

Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2001.Includes bibliographical references (p. 53-55).by Frank Dabek.M.Eng

Separation of carbonyl 2,4-dinitrophenylhydrazones by capillary electrochromatography with diode array detection

The applicability of capillary electrochromatography (CEC) with photodiode array detection for the analysis of carbonyl hydrazones is presented. The CEC separation of thirteen hydrazones was optimized by a systematic variation of conditions using a commercially available CE system and a 3-μm porous C18-bonded silica capillary column. The separation profile obtained under optimal isocratic conditions (60% actonitrile-4% tetrahydrofuran-5 mM Tris, pH 8) is similar to those reported for gradient HPLC, with significant improvements in efficiency (to 150 000-250 000 theoretical plates/m) and analysis time (by a factor of 4). The retention time reproducibility is better than 0.2% (RSD) from run to run and 1% from day to day. The limits of detection for individual carbonyl hydrazones range between 0.1 and 0.5 μg/ml. Applications to ambient air and automobile exhaust are shown. Copyright (C) 1999 Elsevier Science B.V

Capillary electrophoresis characterization of molecularly imprinted polymer particles in fast binding with 17β-estradiol

Molecularly imprinted polymer (MIP) submicron particles were synthesized, using either ethylene glycol dimethacrylate or trimethylolpropane trimethacrylate as a cross-linker, specifically for recognition of 17β-estradiol (E2). HPLC with fluorescence detection (HPLC-FD) results showed that 90(±5)% of E2 bound onto these particles after 2 min of incubation, and 96(±3)% after long equilibrium. The binding capacity was 8(±3) μmol/g for MIP particles prepared using ethylene glycol dimethacrylate, and 33-43(±8) μmol/g for using trimethylolpropane trimethacrylate. CE separation of MIP and non-imprinted polymer particles was successful when 50mM borate buffer (pH 8.5) containing 0.005% w/v EOTrol™ LN in reverse polarity (-30 kV) was used. The electrophoretic mobilities of MIP and non-imprinted polymer particles, together with dynamic light scattering measurement of particle sizes, allowed for an estimation of their surface charges. Automated injection of E2 and particles in mixture set a lower limit of 20(±1) s on incubation time for the study of fast binding kinetics. The presence of E2 and bisphenol A (BPA) together tested the selectivity of MIP particles, when the two compounds competed for available binding cavities or sites. Addition of E2 after BPA confirmed E2 occupation of the specific binding cavities, via displacement of BPA

Molecularly-imprinted polypyrrole-modified stainless steel frits for selective solid phase preconcentration of ochratoxin A

A molecularly-imprinted polymer (MIP) was prepared by electropolymerization of pyrrole (Py) onto a stainless steel frit, using ochratoxin A (OTA) as the template, in order to make a micro solid phase preconcentration (μSPP) device. The OTA template was removed with 1% triethylamine (TEA) in methanol. Compared to non-imprinted polypyrrole (PPy), the molecularly-imprinted polypyrrole (MIPPy) enhanced the selective binding of OTA. The percentage recovery improved from 0 to 40% when the OTA sample solution was acidified with 1 M HCl (1% by volume). At a flow rate of 0.2 mL/min, maximum OTA binding was reached in 6 min after a total loading of 3.2 ng OTA. Final elution of the OTA was analyzed by high performance liquid chromatography (HPLC) with fluorescence detection, using 20:80 v/v acetonitrile-ammonia buffer (NH 4Cl/NH 3, 20 mM, pH 9.2) as the mobile phase. The MIPPy-μSPP-HPLC results clearly demonstrated that the MIPPy-μSPP device afforded selective preconcentration of OTA from red wine samples, at OTA concentration levels as low as 0.05 ppb, prior to HPLC analysis

Speciation of mercury at ng/ml concentration levels by capillary electrophoresis with amperometric detection

A technique based on capillary electrophoresis and amperometric detection (CE-AD) has been developed for the speciation of mercury. This technique has the unique capability to detect only cationic mercury species that are electrochemically active. Capillary electrophoresis with electrokinetic injection allows efficient separation of inorganic mercury and organomercury cations in 8min. Selective detection of these electrochemically active species is attained by controlling the reduc