Effect of electrolyte composition on micro-arc anodization of AM60B magnesium alloy

Anodic oxidation in micro-arc regime is one of the most investigated techniques used to coat magnesium alloys with ceramic coatings for protection from corrosion and wear. The anodization electrolyte composition affects the current/voltage characteristic of the cell, the anodic oxide layer morphology and its behaviour in aggressive environment. In this paper the influence of the electrolyte composition on the anodic oxidation process and oxide properties is discussed. Scanning electron microscopy, x- ray diffraction, and x- ray photoelectron spectroscopy were employed to assess morphology, crystallographic structure and composition of the anodic oxide. Electrochemical polarization tests were performed to evaluate the corrosion resistance behaviour of the coated magnesium alloys

Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion

Wnt signaling is important in development and can also contribute to the initiation and progression of cancer. The Secreted Frizzled Related Proteins (SFRPs) constitute a family of Wnt modulators, crucial for controlling Wnt signaling. Here we investigate the expression and role of SFRP3 in melanoma

A decade of detailed observations (2008-2018) in steep bedrock permafrost at the Matterhorn Hörnligrat (Zermatt, CH)

The PermaSense project is an ongoing interdisciplinary effort between geo-science and engineering disciplines and started in 2006 with the goals of realizing observations that previously have not been possible. Specifically, the aims are to obtain measurements in unprecedented quantity and quality based on technological advances. This paper describes a unique >10-year data record obtained from in situ measurements in steep bedrock permafrost in an Alpine environment on the Matterhorn Hörnligrat, Zermatt, Switzerland, at 3500ma:s:l. Through the utilization of state-of-the-art wireless sensor technology it was possible to obtain more data of higher quality, make these data available in near real time and tightly monitor and control the running experiments. This data set (https://doi.org/10.1594/PANGAEA.897640,Weber et al., 2019a) constitutes the longest, densest and most diverse data record in the history of mountain permafrost research worldwide with 17 different sensor types used at 29 distinct sensor locations consisting of over 114.5 million data points captured over a period of 10 or more years. By documenting and sharing these data in this form we contribute to making our past research reproducible and facilitate future research based on these data, e.g., in the areas of analysis methodology, comparative studies, assessment of change in the environment, natural hazard warning and the development of process models. Finally, the cross-validation of four different data types clearly indicates the dominance of thawing-related kinematics

Dvl2-Dependent Activation of Daam1 and RhoA Regulates Wnt5a-Induced Breast Cancer Cell Migration

The Dishevelled (Dvl) and Dishevelled-associated activator of morphogenesis 1 (Daam1) pathway triggered by Wnt5a regulates cellular polarity during development and tissue homoeostasis. However, Wnt5a signaling in breast cancer progression remains poorly defined.We showed here that Wnt5a activated Dvl2, Daam1 and RhoA, and promoted migration of breast cancer cells, which was, however, abolished by Secreted Frizzled-related protein 2 (sFRP2) pretreatment. Dominant negative Dvl2 mutants or Dvl2 siRNA significantly decreased Wnt5a-induced Daam1/RhoA activation and cell migration. Ectopic expression of N-Daam1, a dominant negative mutant, or Daam1 siRNA remarkably inhibited Wnt5a-induced RhoA activation, stress fiber formation and cell migration. Ectopic expression of dominant negative RhoA (N19) or C3 exoenzyme transferase, a Rho inhibitor, decreased Wnt5a-induced stress fiber formation and cell migration.Taken together, we demonstrated for the first time that Wnt5a promotes breast cancer cell migration via Dvl2/Daam1/RhoA

Maternal high fat diet compromises survival and modulates lung development of offspring, and impairs lung function of dams (female mice)

© 2019 The Author(s). Published in Respiratory Research. Background: Epidemiological studies have identified strong relationships between maternal obesity and offspring respiratory dysfunction; however, the causal direction is not known. We tested whether maternal obesity alters respiratory function of offspring in early life. Methods: Female C57Bl/6 J mice were fed a high or low fat diet prior to and during two rounds of mating and resulting pregnancies with offspring lung function assessed at 2 weeks of age. The lung function of dams was measured at 33 weeks of age. Results: A high fat diet caused significant weight gain prior to conception with dams exhibiting elevated fasting glucose, and glucose intolerance. The number of surviving litters was significantly less for dams fed a high fat diet, and surviving offspring weighed more, were longer and had larger lung volumes than those born to dams fed a low fat diet. The larger lung volumes significantly correlated in a linear fashion with body length. Pups born from the second pregnancy had reduced tissue elastance compared to pups born from the first pregnancy, regardless of the dam's diet. As there was reduced offspring survival born to dams fed a high fat diet, the statistical power of lung function measures of offspring was limited. There were signs of increased inflammation in the bronchoalveolar lavage fluid of dams (but not offspring) fed a high fat diet, with more tumour necrosis factor-α, interleukin(IL)-5, IL-33 and leptin detected. Dams that were fed a high fat diet and became pregnant twice had reduced fasting glucose immediately prior to the second mating, and lower levels of IL-33 and leptin in bronchoalveolar lavage fluid. Conclusions: While maternal high fat diet compromised litter survival, it also promoted somatic and lung growth (increased lung volume) in the offspring. Further studies are required to examine downstream effects of this enhanced lung volume on respiratory function in disease settings