American Thoracic Society 2019 Pediatric Core Curriculum

The American Thoracic Society Pediatric Core Curriculum updates clinicians annually in pediatric pulmonary disease in a 3 to 4 year recurring cycle of topics. The 2019 course was presented in May during the Annual International Conference. An American Board of Pediatrics Maintenance of Certification module and a continuing medical education exercise covering the contents of the Core Curriculum can be accessed online at www.thoracic.org.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152541/1/ppul24482_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/152541/2/ppul24482.pd

Sox6 Is Necessary for Efficient Erythropoiesis in Adult Mice under Physiological and Anemia-Induced Stress Conditions

BACKGROUND: Definitive erythropoiesis is a vital process throughout life. Both its basal activity under physiological conditions and its increased activity under anemia-induced stress conditions are highly stimulated by the hormone erythropoietin. The transcription factor Sox6 was previously shown to enhance fetal erythropoiesis together and beyond erythropoietin signaling, but its importance in adulthood and mechanisms of action remain unknown. We used here Sox6 conditional null mice and molecular assays to address these questions. METHODOLOGY/PRINCIPAL FINDINGS: Sox6fl/flErGFPCre adult mice, which lacked Sox6 in erythroid cells, exhibited compensated anemia, erythroid cell developmental defects, and anisocytotic, short-lived red cells under physiological conditions, proving that Sox6 promotes basal erythropoiesis. Tamoxifen treatment of Sox6fl/flCaggCreER mice induced widespread inactivation of Sox6 in a timely controlled manner and resulted in erythroblast defects before reticulocytosis, demonstrating that impaired erythropoiesis is a primary cause rather than consequence of anemia in the absence of Sox6. Twenty five percent of Sox6fl/flErGFPCre mice died 4 or 5 days after induction of acute anemia with phenylhydrazine. The others recovered slowly. They promptly increased their erythropoietin level and amplified their erythroid progenitor pool, but then exhibited severe erythroblast and reticulocyte defects. Sox6 is thus essential in the maturation phase of stress erythropoiesis that follows the erythropoietin-dependent amplification phase. Sox6 inactivation resulted in upregulation of embryonic globin genes, but embryonic globin chains remained scarce and apparently inconsequential. Sox6 inactivation also resulted in downregulation of erythroid terminal markers, including the Bcl2l1 gene for the anti-apoptotic factor Bcl-xL, and in vitro assays indicated that Sox6 directly upregulates Bcl2l1 downstream of and beyond erythropoietin signaling. CONCLUSIONS/SIGNIFICANCE: This study demonstrates that Sox6 is necessary for efficient erythropoiesis in adult mice under both basal and stress conditions. It is primarily involved in enhancing the survival rate and maturation process of erythroid cells and acts at least in part by upregulating Bcl2l1

Cluster and Sentiment Analyses of YouTube Textual Feedback of Programming Language Learners to Enhance Learning in Programming

This study intends to determine the clusters and sentiments of feedback of YouTube users in learning to program in Python and C++. Toward this goal, a total of 2,583 feedback on introductory video tutorials about Python and C++ were collected. It is found that the words “thanks” and “thank” were the most frequently occurring word in both YouTube videos – indicating appreciation and helpfulness of the video tutorials. The results of k-means cluster analyses further disclosed that groups of feedback are similar across the two languages, i.e., confirmation, helpfulness, gratitude, and recommendation. YouTube users expressed positive sentiments towards the tutorial videos. Implications to teaching programming and YouTube video content development are presented. Limitations of the study are also offered

Unsupervised Streaming Feature Selection in Social Media

The explosive growth of social media sites brings about mas-sive amounts of high-dimensional data. Feature selection is effective in preparing high-dimensional data for data an-alytics. The characteristics of social media present novel challenges for feature selection. First, social media data is not fully structured and its features are usually not prede-fined, but are generated dynamically. For example, in Twit-ter, slang words (features) are created everyday and quickly become popular within a short period of time. It is hard to directly apply traditional batch-mode feature selection methods to find such features. Second, given the nature of social media, label information is costly to collect. It ex-acerbates the problem of feature selection without knowing feature relevance. On the other hand, opportunities are also unequivocally present with additional data sources; for ex-ample, link information is ubiquitous in social media and could be helpful in selecting relevant features. In this paper, we study a novel problem to conduct unsupervised streaming feature selection for social media data. We investigate how to exploit link information in streaming feature selection, re-sulting in a novel unsupervised streaming feature selection framework USFS. Experimental results on two real-world so-cial media datasets show the effectiveness and efficiency of the proposed framework comparing with the state-of-the-art unsupervised feature selection algorithms