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Not AvailableTobacco grown in vertisols was investigated for endophytic Mycoflora as a possible source of bio protection to the host against insect pests, pathogens and even domestic herbivores. There are 65 Nicotiana sp. available in the world, of which 45 are being maintained in India. Several of these species have resistance to pests and diseases and are being used for developing disease/ pest resistant tobacco varieties. One hundred sixty segments from 30 plants of Nicotiana tabacum L. collected from different locations of FCV tobacco grown in vertisols were processed for the presence of endophytic fungi. A total of 9 fungal species viz. Aspergillus flavus, A. niger, Asperigillus sp, Penicillium sp. were isolated. Among the endophytic flora, Aspergillus was the most prominent genus.Not Availabl

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Not AvailableNew method for the selective isolation of actinomycetes from natural mixed microbial populations is described. A nutrient agar medium was overlaid with a 0.22-0.45μm pore cellulose ester membrane filter, and the surface of the filter was inoculated. During incubation, the branched mycelia of the actinomycetes penetrated the filter pores to the underlying agar medium, whereas growth of non actinomycete bacteria was restricted to the filter surface. The membrane filter was removed, and the agar medium was re incubated to allow the development of the isolated actinomycete colonies. This procedure selects actinomycetes on the basis of their characteristic mycelial mode of growth, offers a general method for their selective isolation, and does not rely on the use of special nutrient media or of antibacterial antibioticsNot Availabl