16 research outputs found
Defining the Viral Ecology of Epizootic Shell Disease of the American Lobster (Homarus Americanus)
Ultrastructural Analysis of the Reactivation of the Murid Herpesvirus 4, Strain 68 (MHV-68) in a Latently-Infected B Cell Line
Making the Invisible Visible: Empowering Pre-college Teachers Through Microscopic Investigations of Micro- and Nano-worlds
Integration of Transmission Electron Microscopy and Extremophile Virology Research into University and K-12 Education in Maine and Kenya
VIROCRINE TRANSFORMATION: The Intersection Between Viral Transforming Proteins and Cellular Signal Transduction Pathways
Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging
Herpesvirus saimiri (HVS) is capable of infecting a range of human cell types with high efficiency and the viral genome persists as high copy number, circular, nonintegrated episomes which segregate to progeny upon cell division. This allows the HVS-based vector to stably transduce a dividing cell population and provide sustained transgene expression for an extended period of time both in vitro and in vivo. Here we assess the dissemination of HVS-based vectors in vivo following intravenous and intraperitoneal administration. Bioluminescence imaging of an HVS-based vector expressing luciferase demonstrates that the virus can infect and establish a persistent latent infection in a variety of mouse tissues. Moreover, the long-term in vivo maintenance of the HVS genome as a nonintegrated circular episome provided sustained expression of luciferase over a 10-week period. A particularly high level of transgene expression in the liver and the ability of HVS to infect and persist in hepatic stellate cells suggest that HVS-based vectors may have potential for the treatment of inherited and acquired liver diseases