31 research outputs found
Molecular characterization of cDNAs corresponding to genes expressed during almond (Prunus amygdalus Batsch) seed development
No full textInsertion of Mu into the Shrunken 1 gene of maize affects transcriptional and post-transcriptional regulation of Sh1 RNA
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Mini-scale method for nuclear run-on transcription assay in plants
No full textWe present a mini-scale method for nuclear run-on transcription assay. In our method, all the centrifuge steps can be carried out by using micro-tubes for short time (5 min each) throughout the process, including isolation of transcriptionally active nuclei and purification of labeled RNA after synthesis of RNA in isolated nuclei. The assay can be performed using a small amount of plant tissue, which enables analysis of developmental changes in transcriptional status of given genes in a single individual plant. Successful results were obtained using the tissues of flower and leaf of petunia and embryo of pea, suggesting that the method is potentially applicable to a variety of plant tissues
Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure
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Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid
No full textSubstances in nuclear wheat germ extracts which interfere with polymerase III transcriptional activity in vitro
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