Marfan syndrome. A case

Se realiza la presentación de un caso atendido en el Servicio de Anestesiología y Reanimación del Hospital General Docente “Dr. Agostinho Neto” de Guantánamo, de una paciente femenina de 46 años con síndrome de Marfán y oclusión intestinal por bridas y adherencias, acompañado de prolapso en válvula mitral diagnosticado hace un año sin tratamiento medicamentoso, fumadora y con asma bronquial. Después de una valoración preoperatoria se realiza anestesia general endotraqueal. La paciente no presentó complicaciones quirúrgicas ni anestésicas. Se obtuvieron buenas condiciones de analgesia durante el postoperatorio. A presentation of a case that was performed and treated at the Anesthesiology Service, General Teaching Hospital "Dr. Agostinho Neto "Guantanamo. A female patient of 46 years with Marfan syndrome and intestinal obstruction due to adhesions and flanges, accompanied by mitral valve prolapsed diagnosed one year ago with no medication, smoking with bronchial  asthma. After preoperative evaluation is applied an endotracheal general anesthesia. The patient had no surgical or anesthetic complications. Good analgesia conditions were obtained postoperatively

El mirall de plata en les fotografies: importància, mecanisme d’aparició i nou procediment d’eliminació

El mirall de plata és una degradació que apareix a la majoria de les fotografies i pel·lícules de cinema històriques amb imatge de plata revelada (D.O.P.) en blanc i negre. La seva eliminació ha representat tants problemes que sovint s’ha descartat. Nosaltres presentem un nou procediment d’eliminació senzill, eficient i estable a llarg termini.L’estudi de les causes d’aparició ens aporta llum sobre aspectes que havien quedat poc explicats, especialment el fet que és una degradació que apareix sempre a la superfície de la imatge, el que ens duu a pensar en un mecanisme de transport de la plata per càrregues elèctriques. Això també pot explicar els incendis espontanis de les pel·lícules de nitrat

Treatment Seeking Problem Gamblers: Characteristics of Individuals who Offend to Finance Gambling

The relationship between Crime and gambling is well established, however few studies have examined offending specifically to finance gambling within a UK gambling treatment-seeking population. 1226 treatment-seeking gamblers completed the Problem Gambling Severity Index (PGSI), Patient Health Questionnaire, and the Generalized Anxiety Disorder 7 item scale, and were asked whether they had committed any illegal behaviours to finance gambling. 42.5 % reported offending behaviour. A greater proportion of the offending group were single or married/ cohabiting, had a lower-level qualifications, lower income, had experienced childhood abuse, family mental health problems and gambling related harms compared to the non-offending group. Offenders reported higher anxiety, depression and disordered gambling scores. Disordered gamblers who offend make up a discrete and complex subgroup with distinct vulnerabilities. Findings will be useful to clinicians involved in the assessment and management of problematic gambling. Gamblers who offend to finance gambling may have different treatment needs and treatment providers should administer appropriate clinical interventions to address vulnerabilities

Promoter Complexity and Tissue-Specific Expression of Stress Response Components in Mytilus galloprovincialis, a Sessile Marine Invertebrate Species

The mechanisms of stress tolerance in sessile animals, such as molluscs, can offer fundamental insights into the adaptation of organisms for a wide range of environmental challenges. One of the best studied processes at the molecular level relevant to stress tolerance is the heat shock response in the genus Mytilus. We focus on the upstream region of Mytilus galloprovincialis Hsp90 genes and their structural and functional associations, using comparative genomics and network inference. Sequence comparison of this region provides novel evidence that the transcription of Hsp90 is regulated via a dense region of transcription factor binding sites, also containing a region with similarity to the Gamera family of LINE-like repetitive sequences and a genus-specific element of unknown function. Furthermore, we infer a set of gene networks from tissue-specific expression data, and specifically extract an Hsp class-associated network, with 174 genes and 2,226 associations, exhibiting a complex pattern of expression across multiple tissue types. Our results (i) suggest that the heat shock response in the genus Mytilus is regulated by an unexpectedly complex upstream region, and (ii) provide new directions for the use of the heat shock process as a biosensor system for environmental monitoring

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field