14 research outputs found
Localization and Characterization of STRO-1+ Cells in the Deer Pedicle and Regenerating Antler
The annual regeneration of deer antlers is a unique developmental event in mammals, which as a rule possess only a very limited capacity to regenerate lost appendages. Studying antler regeneration can therefore provide a deeper insight into the mechanisms that prevent limb regeneration in humans and other mammals, and, with regard to medical treatments, may possibly even show ways how to overcome these limitations. Traditionally, antler regeneration has been characterized as a process involving the formation of a blastema from de-differentiated cells. More recently it has, however, been hypothesized that antler regeneration is a stem cell-based process. Thus far, direct evidence for the presence of stem cells in primary or regenerating antlers was lacking. Here we demonstrate the presence of cells positive for the mesenchymal stem cell marker STRO-1 in the chondrogenic growth zone and the perivascular tissue of the cartilaginous zone in primary and regenerating antlers as well as in the pedicle of fallow deer (Dama dama). In addition, cells positive for the stem cell/progenitor cell markers STRO-1, CD133 and CD271 (LNGFR) were isolated from the growth zones of regenerating fallow deer antlers as well as the pedicle periosteum and cultivated for extended periods of time. We found evidence that STRO-1+ cells isolated from the different locations are able to differentiate in vitro along the osteogenic and adipogenic lineages. Our results support the view that the annual process of antler regeneration might depend on the periodic activation of mesenchymal progenitor cells located in the pedicle periosteum. The findings of the present study indicate that not only limited tissue regeneration, but also extensive appendage regeneration in a postnatal mammal can occur as a stem cell-based process
Zebrafish: a vertebrate tool for studying basal body biogenesis, structure, and function.
Understanding the role of basal bodies (BBs) during development and disease has been largely overshadowed by research into the function of the cilium. Although these two organelles are closely associated, they have specific roles to complete for successful cellular development. Appropriate development and function of the BB are fundamental for cilia function. Indeed, there are a growing number of human genetic diseases affecting ciliary development, known collectively as the ciliopathies. Accumulating evidence suggests that BBs establish cell polarity, direct ciliogenesis, and provide docking sites for proteins required within the ciliary axoneme. Major contributions to our knowledge of BB structure and function have been provided by studies in flagellated or ciliated unicellular eukaryotic organisms, specifically Tetrahymena and Chlamydomonas. Reproducing these and other findings in vertebrates has required animal in vivo models. Zebrafish have fast become one of the primary organisms of choice for modeling vertebrate functional genetics. Rapid ex-utero development, proficient egg laying, ease of genetic manipulation, and affordability make zebrafish an attractive vertebrate research tool. Furthermore, zebrafish share over 80Â % of disease causing genes with humans. In this article, we discuss the merits of using zebrafish to study BB functional genetics, review current knowledge of zebrafish BB ultrastructure and mechanisms of function, and consider the outlook for future zebrafish-based BB studies
The Effect of Surgical Approach on Femoral Stem Position in Canine Cemented Total Hip Replacement
Cyclic activation and inactivation of brain vessels involving inflammatory mediators — implications for stroke
Depressive symptoms in long term care facilities in Western Canada: a cross sectional study
Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice
Direct Renin Inhibition with Aliskiren Improves Ischemia-Induced Neovasculogenesis in Diabetic Animals via the SDF-1 Related Mechanism
Global Gene Expression Profiling of Endothelium Exposed to Heme Reveals an Organ-Specific Induction of Cytoprotective Enzymes in Sickle Cell Disease
Emotionality and intentionality in bonobo playful communication
Great apes show very complex systems for
communicating emotions and intentions. Whereas gestures
are intentional signals, facial expressions can disclose both
emotions and intentions. The playful context is a good field
to explore the possible dichotomy between intentionally
and emotionally driven signals as it has been suggested that
one of its functions is to learn producing and decoding
communicative patterns. To understand how signals are
produced during play and how they are modified in the
course of ontogeny, we investigated the use of playful
facial expressions and gestures in bonobos (Pan paniscus),
a tolerant species showing a high propensity to play even as
adults. Our results showed that the use of play faces and
gestures is strongly influenced by the characteristics of the
play session. Both play faces and gestures were more often
performed when social play involved physical contact and
when the receiver was visually attending, thus suggesting
that both signals can be strategically employed when
communicating becomes more urgent. Compared to play
faces, gestures were more frequent during dyadic than
polyadic sessions, when a unique receiver was involved.
Being gestures not context specific, they are probably used
more selectively by the sender. On the contrary, play faces
are context specific and transmit an unequivocal positive
message that cannot be misconceived. These features
legitimize a broad use of playful facial expressions, independently
of the number of playmates. The similarities and
differences in the production of these signals are probably
linked to the different degree of emotionality and intentionality
characterizing them