Profil phytochimique, pharmaco-biologique et cytotoxique des feuilles de Uvariodendron molundense (Annonaceae)

The aim of present study was to investigate and evaluate the phytochemical of the anti-sickle cell, anti-inflammatory, anti-free radical and cytotoxic activities of the leaves of Uvariodendron molundense, a Non-timber forest product (NTFP). The study found that the leaf powder of this NTFP contains the characteristic histological elements such as parenchyma fragments, calcium oxalate crystals, fibres, ringed vessels, starch grains and punctate vessel fragments. It also contains tannins, saponosides, flavonoids, phenolic acids, iridoids, anthocyanins, anthrones, anthraquinones and terpenes. The content of total polyphenols is 442 ±1.1 mg GAE/g extract and that of total flavonoids is 7.0 ± 0.18 mg QE/g extract. The antiradical activity of the percolate is higher than that of the decoctate and U. molundense leaves are not cytotoxic (%Hemolysis <50 to 1000 µg/mL). These leaves have anti-inflammatory properties that are low for the decoction (%I=35.8 ± 4.9) and medium for the percolate (%I=50 ±3.3). Both the decoctate and the percolate of U. molundense leaves have in vitro antisickling properties. It is therefore desirable that further phytochemical studies be carried out on the leaves of U. molundense to identify the active molecules. Keywords: Uvariodendron molundense, Non-timber forest product, medicinal product, sickle cell disease, traditional medicineLa présente étude a pour but l’étude phytochimique et évaluation des activités antidrépanocytaire, anti-inflammatoire, antiradicalaire et cytotoxique des feuilles de Uvariodendron molundense, un produit forestier non ligneux (PFNL). Il ressort de cette étude que la poudre des feuilles de ce PFNL contient les éléments histologiques caractéristiques tels que les fragments de parenchyme, les cristaux d’oxalates de calcium, les fibres, les vaisseaux annelés, les grains d’amidon et les fragments de vaisseaux ponctués. Il contient également les tanins, les saponosides, les flavonoïdes, les acides phénols, les iridoïdes, les anthocyanes, les anthrones, les anthraquinones et les terpènes. La teneur en polyphénols totaux est de 442 ± 1,1 mg EAG/g d’extrait et tandis que celle des flavonoïdes totaux est de 7,0 ± 0,18 mg EQ/g d’extrait. L’activité antiradicalaire du percolât est supérieure à celle du décocté et les feuilles de U. molundense ne sont pas cytotoxique (%Hemolyse <50 à 1000 µg/mL). Ces feuilles ont des propriétés anti-inflammatoires qui est cependant faible pour le décocté (%I=35,8 ± 4,9) et moyenne pour le percolât (%I = 50 ± 3,3). Le décocté et le percolât des feuilles de U. molundense ont des propriétés antifalcémiantes in vitro. Il est donc souhaitable qu’une étude phytochimique plus approfondie soit réalisée sur les feuilles de U. molundense afin d’identifier ses molécules actives. Mots clés: Uvariodendron molundense, Produit forestier non ligneux, alicament, drépanocytose, Médecine Traditionnell

Profil phytochimique, pharmaco-biologique et cytotoxique des feuilles de Uvariodendron molundense (Annonaceae)

La présente étude a pour but l’étude phytochimique et évaluation des activités antidrépanocytaire, anti-inflammatoire, antiradicalaire et cytotoxique des feuilles de Uvariodendron molundense, un produit forestier non ligneux (PFNL). Il ressort de cette étude que la poudre des feuilles de ce PFNL contient les éléments histologiques caractéristiques tels que les fragments de parenchyme, les cristaux d’oxalates de calcium, les fibres, les vaisseaux annelés, les grains d’amidon et les fragments de vaisseaux ponctués. Il contient également les tanins, les saponosides, les flavonoïdes, les acides phénols, les iridoïdes, les anthocyanes, les anthrones, les anthraquinones et les terpènes. La teneur en polyphénols totaux est de 442 ± 1,1 mg EAG/g d’extrait et tandis que celle des flavonoïdes totaux est de 7,0 ± 0,18 mg EQ/g d’extrait. L’activité antiradicalaire du percolât est supérieure à celle du décocté et les feuilles de U. molundense ne sont pas cytotoxique (%Hemolyse <50 à 1000 µg/mL). Ces feuilles ont des propriétés anti-inflammatoires qui est cependant faible pour le décocté (%I=35,8 ± 4,9) et moyenne pour le percolât (%I = 50 ± 3,3). Le décocté et le percolât des feuilles de U. molundense ont des propriétés antifalcémiantes in vitro. Il est donc souhaitable qu’une étude phytochimique plus approfondie soit réalisée sur les feuilles de U. molundense afin d’identifier ses molécules actives. Mots clés: Uvariodendron molundense, Produit forestier non ligneux, alicament, drépanocytose, Médecine Traditionnell

Profil phytochimique, pharmaco-biologique et cytotoxique des feuilles de Uvariodendron molundense (Annonaceae)

The aim of present study was to investigate and evaluate the phytochemical of the anti-sickle cell, anti-inflammatory, anti-free radical and cytotoxic activities of the leaves of Uvariodendron molundense, a Non-timber forest product (NTFP). The study found that the leaf powder of this NTFP contains the characteristic histological elements such as parenchyma fragments, calcium oxalate crystals, fibres, ringed vessels, starch grains and punctate vessel fragments. It also contains tannins, saponosides, flavonoids, phenolic acids, iridoids, anthocyanins, anthrones, anthraquinones and terpenes. The content of total polyphenols is 442 ±1.1 mg GAE/g extract and that of total flavonoids is 7.0 ± 0.18 mg QE/g extract. The antiradical activity of the percolate is higher than that of the decoctate and U. molundense leaves are not cytotoxic (%Hemolysis &lt;50 to 1000 µg/mL). These leaves have anti-inflammatory properties that are low for the decoction (%I=35.8 ± 4.9) and medium for the percolate (%I=50 ±3.3). Both the decoctate and the percolate of U. molundense leaves have in vitro antisickling properties. It is therefore desirable that further phytochemical studies be carried out on the leaves of U. molundense to identify the active molecules. Keywords: Uvariodendron molundense, Non-timber forest product, medicinal product, sickle cell disease, traditional medicineLa présente étude a pour but l’étude phytochimique et évaluation des activités antidrépanocytaire, anti-inflammatoire, antiradicalaire et cytotoxique des feuilles de Uvariodendron molundense, un produit forestier non ligneux (PFNL). Il ressort de cette étude que la poudre des feuilles de ce PFNL contient les éléments histologiques caractéristiques tels que les fragments de parenchyme, les cristaux d’oxalates de calcium, les fibres, les vaisseaux annelés, les grains d’amidon et les fragments de vaisseaux ponctués. Il contient également les tanins, les saponosides, les flavonoïdes, les acides phénols, les iridoïdes, les anthocyanes, les anthrones, les anthraquinones et les terpènes. La teneur en polyphénols totaux est de 442 ± 1,1 mg EAG/g d’extrait et tandis que celle des flavonoïdes totaux est de 7,0 ± 0,18 mg EQ/g d’extrait. L’activité antiradicalaire du percolât est supérieure à celle du décocté et les feuilles de U. molundense ne sont pas cytotoxique (%Hemolyse &lt;50 à 1000 µg/mL). Ces feuilles ont des propriétés anti-inflammatoires qui est cependant faible pour le décocté (%I=35,8 ± 4,9) et moyenne pour le percolât (%I = 50 ± 3,3). Le décocté et le percolât des feuilles de U. molundense ont des propriétés antifalcémiantes in vitro. Il est donc souhaitable qu’une étude phytochimique plus approfondie soit réalisée sur les feuilles de U. molundense afin d’identifier ses molécules actives. Mots clés: Uvariodendron molundense, Produit forestier non ligneux, alicament, drépanocytose, Médecine Traditionnell

Ethnobotanical Study and Vulnerability of Uvariodendron molundense (Annonaceae) in Gbado-Lite City (Ubangi Eco-region), Democratic Republic of the Congo

Democratic Republic of the Congo is a real reservoir of medicinal plants. These plants play a major role in the treatment of certain common pathologies in tropical regions. The aim of this study was to list the ethnomedical uses of Uvariodendron molundense, a medicinal and aromatic plant from the Ubangi ecoregion. The ethnobotanical survey was carried out in Gbado-Lite with 200 people using stratified probability sampling. The respondents were interviewed individually on the basis of free consent. The study revealed that the majority of respondents were men (72%) and had a secondary education (37%), followed respectively by illiterates (34%) and those with a primary education (28%), and finally, university graduates represented only 1% of the respondents. 83% of the respondents were farmers, while 79% of the respondents were married. The leaf is the most used organ (81%) followed by stem and root bark. U. molundense is used both as food and medicine. The plant treats eight diseases (pain, malaria, cold, hypertension, gastritis, infection, headache and rheumatism). The calculated value of the informant consensus factor is 0.96 and indicates that there is a high degree of consensus among informants regarding the use of U. molundense against these diseases in Gbado-Lite. Decoction is the most commonly used method of preparation (89%) and 50% of the respondents stated that the species is currently not very abundant, while 31% of the respondents felt that the plant was rare. The calculated value of the vulnerability index shows that U. molundense is very vulnerable in its natural environment (Iv ˃ 2.5)

Epidemiological and clinical profile of vulvovaginitis at the University Clinics of Kisangani City (Tshopo Province), Democratic Republic of the Congo

Introduction Vulvovaginitis is an inflammation that affects both the vulva (the external part of the female genital organs) and the vagina. It can be caused by various factors such as bacterial, fungal, or viral infections, allergic or irritative reactions, or hormonal imbalances. Common symptoms include itching, burning, redness, pain, and abnormal vaginal discharge. Purpose This study aimed to examine the epidemiological and clinical approach to vulvovaginitis at the University Clinics of Kisangani during the years 2012-2014. Methods The study included a total of 1,966 patients admitted to the Gynaecology and Obstetrics Department during the study period. Data on age, marital status, education level, occupation, and residential area were collected. Information on antecedent infections, presenting symptoms, and causative agents was also gathered. Statistical analysis was performed to determine the prevalence and distribution of vulvovaginitis cases. Results Out of the total patients, 298 (15.2%) were diagnosed with vulvovaginitis. Most cases were aged between 20-24 years (29.9%), followed by 15-19 years (25.8%), and 25-29 years (17.1%). Most cases were married (55.4%) and had secondary education (46%). Housewives accounted for the highest proportion of cases (44.63%). Makiso had the highest number of cases (54%), followed by Mangobo (25.2%). The most common antecedent infection was vaginal candidiasis (46.3%), and the most common presenting symptom was abnormal genital discharge (37.2%). Candida albicans was the most common causative agent (46.64%), followed by Neisseria gonorrheae (26.84%) and Trichomonas vaginalis (22.48%). Other agents were Chlamydia trachomatis (1.67%), Haemophilus ducreyi (1%), human papillomavirus (HPV), and herpes simplex virus (HSV) (0.67% each). Conclusion Vulvovaginitis is a common condition at the University Clinics of Kisangani, affecting a significant proportion of women. The findings highlight the need for further research and education on the prevention and management of vulvovaginitis in this population