Preventive and Curative Effects of Atriplex Halimus L. Aqueous Extract on Benzene Provoked Hepatic Injury in Rats

Objective: The present study was designed to estimate the preventive and curative potency of aqueous extract of Atriplex halimus L. (Ah) aerial parts against benzene (BZ) intoxication in liver rats. Methods: 30 male albino rats were divided into five groups of 6 rats each: Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in drinking water for 15 weeks. Aqueous extract of Atriplex halimus was received intragastrically during the last 30 days of BZ exposure for curative treatment (AhC) and all the duration of BZ exposure for preventive treatment (AhP). At the end the experiment, body weight gain and relative liver weight were estimated and liver enzyme markers (AST, ALT, ALP and LDH) were analysed by spectrophotometry. Histopathological studies on hepatic tissue were also performed by the method of Hematoxylin and Eosin staining. Results: Benzene administrated to rats caused an alteration in physiological parameters (body and organs weigh) and hepatic enzymes markers (AST, ALT, ALP and LDH). In addition, Histopathological studies showed a massive degeneration in liver tissue in BZ-exposed rats. However, treatment by Atriplex halimus especially preventive effect ameliorated most of the adverse effects induced by benzene. Ah restored the altered of physiological, biochemical and histopathological changes. Conclusion: The present study suggests that Atriplex halimus extract could be a substantially promising hepatoprotective agent against benzene toxic effects and may be against other hepatotoxic chemical or drugs. Keywords: Atriplex halimus, Benzene, hepatotoxicity, Rats

PROTECTIVE ROLE OF AQUEOUS EXTRACT OF ATRIPLEX HALIMUS L. AGAINST BENZENE-INDUCED DAMAGE ON RENAL FUNCTION AND GLOMERULAR CELLS IN RATS

Objective: Benzene (BZ) is an important pollutant compound, present in both occupational and general environment that has been linked to adverse health effect. The aim of the current study was to investigate the curative and preventive effect of Atriplex halimus L. (Ah) extract against BZ-induced nephrotoxicity in rats. Methods: A total of 30 male albino Wistar rats were divided into five groups (n=6): Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in their drinking water for 15 weeks. Ah (Aqueous Extract of aerial parts) was given by gavage during the past 30 days of BZ-exposed for curative treatment (AhC) and all the duration of BZ exposure in the animals for preventive treatment (AhP). Some biochemical, oxidative stress parameters, and histopathology of kidney tissue were studied. Results: Obtained results revealed that BZ exposure to rats caused a significant elevation in urea, creatinine, and malondialdehyde levels. Then, it led to reducing the glutathione level, catalase, and glutathione-S-transferase activities when compared to the control animals. Histopathological studies showed a massive damage in kidneys of the BZ-exposed rat. Our results showed that curative treatment of aqueous extract of Ah has a partial correction of the biochemical, oxidative stress parameters, and kidney morphology, but preventive treatment has a dramatic effect on the previous parameters. Conclusion: Results demonstrated the beneficial effects of Ah preventive treatment against stress oxidative and kidney damage induced by BZ

Antioxidant and hepatoprotective Potential of Coriandrum sativum L. against hepatic injury by Lambda-cyhalothrin insecticide

The objective of this study is to evaluate the antioxidant and hepatoprotective activity of aerial part and seeds of Coriandrum sativum plant against Lambda cyhalothrin insecticide.  Male Wistar Albinos rats were randomly divided into control, LCT, CsA, CsS, CsS+LCT, CsA+LCT groups, after 90 days of treatments Biochemical, some oxidative stress parameters, and histopathology of liver tissue were evaluated. Total polyphenol content in aerial part and the seed extract estimated at 9.29 and 14.64 mg EAG / mg of extract and IC50 for an antioxidant activity equal to 19.38 and 22.62 mg/ml respectively. The obtained results revealed that rats received Lambda cyhalothrin insecticide showed a significant change in enzymes activity (AST, ALT, ALP and c-GT) and Glutathione (GSH) in liver. Meanwhile content of hepatic Malondialdehyde (MDA). Histopathology examination of liver revealed that Coriandrum sativum attenuate the incidence of liver lesions triggered by Lambda cyhalothrin intoxication. Therefore, the results of this study show that Coriandrum sativum can be proposed to protect the liver against Lambda cyhalothrin induced oxidative damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenging effect. Keywords: hepatoprotective, antioxidant, Coriandrum sativum L., Lambda cyhalothrin, Oxidative stress

Traditional Herbal Remedies from Algeria for Treating Digestive Disorders

Due to their efficiency in many treatments, the employment of herbal remedies has retained its importance for leading a human life. Traditional knowledge is passed from one generation to the next, which is protected. This research aimed to analyze traditional herbal remedies used in treating digestive disorders by El-Oued people (Southeastern Algeria). The research was conducted from September 2021 to June 2022. One hundred individuals, including thirty plant vendors, were selected from different regions. Personal interviews and questionnaires were employed to collect the data. SPSS version 20 and Microsoft Office Excel 2007 were used to organize the data and conduct the descriptive statistics analysis. This investigation enabled the discovery of 40 species in 16 families and different genres. Lamiaceae, with 22.5 % plants, were the most used sources for the treatment, and 54 % of herbs were spontaneous species. The use of seeds at 21 %, dry parts at 92 % and infusion method at 48 % was most commonly used in herbal remedies preparations. The participants cited Trigonella tibetana, Juniperus communis, and Punica granatum as sources of digestive disorders treatment. El-Oued region has an extremely high number of herbal remedy species, which suggests that the region's traditional medicine serves as a source of knowledge about medicinal plants for treating digestive disorders and illnesses associated with them. Keywords: Herbal remedies, Digestive disorders, Medicinal plants, Interviews