15 research outputs found

    Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis

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    To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO2-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-intuitively, more chlorophyll on a per cell basis. We found that phototrophic cells are less impacted by iron deficiency and this correlates with their higher iron content on a per cell basis, suggesting a greater capacity/ability for iron assimilation in this metabolic state. Phototrophic cells maintain both photosynthetic and respiratory function and their associated Fe-containing proteins in conditions where heterotrophic cells lose photosynthetic capacity and have reduced oxygen evolution activity. Maintenance of NPQ capacity might contribute to protection of the photosynthetic apparatus in iron-limited phototrophic cells. Acetate-grown iron-limited cells maintain high growth rates by suppressing photosynthesis but increasing instead respiration. These cells are also able to maintain a reduced plastoquinone pool

    Ion beam sputtering deposition of silver nanoparticles and TiOx/ZnO nanocomposites for use in surface enhanced vibrational spectroscopy (SERS and SEIRAS)

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    Hybrids consisting of silver nanoparticles (in varying fractions) and of TiOx/ZnO were prepared via top-down ion beam sputtering (IBS) deposition on silicon substrates. The deposited nanomaterials were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. It is shown that such composites represent a viable substrate for use in both surface enhanced Raman spectroscopy (SERS) and surface enhanced infrared absorption spectroscopy (SEIRAS), as exemplarily shown for crystal violet as the model analyte. The C-H bending mode at about 1181 cmâ1and the C-N vibration at 1361 cmâ1observed in the SERS and SEIRAS spectra, respectively, have been used as analytical signal. The substrate consisting of TiOx NPs with 33% fraction of silver provides the strongest enhancement in SERS (up to 10,000-fold), while TiOx/AgNPs with thickness of 2 and 1 nm in ion beam sputtering, respectively, provides the best sensitivity in SEIRAS. The substrates also display photocatalytic activity as shown by the degradation of adsorbed crystal violet under ultraviolet irradiation

    Intrapersonal and Interpersonal Concomitants of Facial Blushing during Everyday Social Encounters

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    Facial blushing may usually be undesirable but may have an ameliorative function for some individuals under some circumstances. Researchers have studied the blush in laboratory settings, but not in daily life. In the present research, conducted with young adults, we employed for the first time an event-contingent recording method for assessing facial blushing during every-day social encounters. Blushing was associated with feeling embarrassed, ashamed, and exposed. These findings, though based on correlational analyses, are consistent with the idea that blushing is often unpleasant and can be maladaptive, and may contribute to the common belief that blushing is an undesirable response. Frequent blushers generally reported lower levels of dominant behavior, higher levels of submissive behavior, and perceived their social interaction partners as more powerful and less affiliative. This was independent of whether they blushed or not, suggesting that altered social behaviors and perceptions are associated with blushing-associated traits rather than with the blushing state. The experience of the blush varied as a function of the frequency with which a person blushed. Blushing was associated with higher levels of shame in frequent blushers than in infrequent blushers. In infrequent blushers, blushing was associated with higher levels of pleasant affect, suggesting that for infrequent blushers the blush may occur in positive social encounters
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