Fluorescent protein tagging confirms the presence of ribosomal proteins atDrosophilapolytene chromosomes

\ud \ud Most ribosomal proteins (RPs) are stoichiometrically incorporated into ribosomal subunits and play essential roles in ribosome biogenesis and function. However, a number of RPs appear to have non-ribosomal functions, which involve direct association with pre-mRNA and transcription factors at transcription sites. The consensus is that the RPs found at these sites are off ribosomal subunits, but observation that different RPs are usually found together suggests that ribosomal or ribosomal-like subunits might be present. Notably, it has previously been reported that antibodies against 20 different RPs stain the same Pol II transcription sites in Drosophila polytene chromosomes. Some concerns, however, were raised about the specificity of the antibodies. To investigate further whether RPs are present at transcription sites in Drosophila, we have generated several transgenic flies expressing RPs (RpS2, RpS5a, RpS9, RpS11, RpS13, RpS18, RpL8, RpL11, RpL32, and RpL36) tagged with either green or red fluorescent protein. Imaging of salivary gland cells showed that these proteins are, as expected, abundant in the cytoplasm as well as in the nucleolus. However, these RPs are also apparent in the nucleus in the region occupied by the chromosomes. Indeed, polytene chromosome immunostaining of a representative subset of tagged RPs confirms the association with transcribed loci. Furthermore, characterization of a strain expressing RpL41 functionally tagged at its native genomic locus with YFP, also showed apparent nuclear accumulation and chromosomal association, suggesting that such a nuclear localization pattern might be a shared feature of RPs and is biologically important. We anticipate that the transgenes described here should provide a useful research tool to visualize ribosomal subunits in Drosophila tissues and to study the non-ribosomal functions of RPs.\u

A search for Low Surface Brightness galaxies in the near-infrared I. Selection of the sample

A sample of about 3,800 Low Surface Brightness (LSB) galaxies was selected using the all-sky near-infrared (J, H and K_s-band) 2MASS survey. The selected objects have a mean central surface brightness within a 5 arcsec radius around their centre fainter than 18 mag/sq.arcsec in the K_s band, making them the lowest surface brightness galaxies detected by 2MASS. A description is given of the relevant properties of the 2MASS survey and the LSB galaxy selection procedure, as well as of basic photometric properties of the selected objects. The latter properties are compared to those of other samples of galaxies, of both LSBs and `classical' high surface brightness (HSB) objects, which were selected in the optical. The 2MASS LSBs have a (B_T_c)-(K_T) colour which is on average 0.9 mag bluer than that of HSBs from the NGC. The 2MASS sample does not appear to contain a significant population of red objects.Comment: accepted for publication in Astronomy and Astrophysics on 24/2/2003; 62 page

Red giant branch stars as probes of stellar populations. II. Properties of the newly discovered globular cluster GLIMPSE-C01

Deep near infrared photometry of the newly discovered Galactic globular Cluster GLIMPSE-C01 is reported. We derived for the first time the metal abundance of this object from the slope of the RGB: [Fe/H]=-1.61+/-0.14 in the scale of Zinn (as implemented in Harris 1996), [Fe/H]=-1.44+/-0.12 in the scale of Caretta & Gratton (1997), and [Fe/H]=-1.12+/-0.12 in the scale of Ferraro et al. (1999). The tip and the clump of the red giant branch were used to confirm the estimates of Kobulnicky et al. (2005), placing the cluster at D~3.7+/-0.8 kpc, behind A_v~15 mag of visual extinction. The best fit to the radial surface brightness profile with a single-mass King's model (1962) yielded core radius r_c=0.78 arcmin, tidal radius r_t=27 arcmin, and central concentration c=1.54. Finally, we estimate the number of the "missing" globulars in the central region of the Milky Way. Based on the spatial distribution of the known clusters, and assuming radial symmetry around the Galactic center, we conclude that the Milky Way contains at least 10+/-3 undiscovered objects. The distribution of known clusters in the bulge seem to resemble the orientation of the Milky Way bar.Comment: A&A accepted; 6 pages, 7 figures (one in jpg format, available upon request from the authors as postscript

The {\alpha}-element enrichment of gas in distant galaxies

The chemical evolution of distant galaxies cannot be assessed from observations of individual stars, in contrast to the case of nearby galaxies. On the other hand, the study of the interstellar medium (ISM) offers an alternative way to reveal important properties of the chemical evolution of distant galaxies. The chemical enrichment of the ISM is produced by all the previous generations of stars and it is possible to precisely determine the metal abundances in the neutral ISM in galaxies. The chemical abundance patterns in the neutral ISM are determined by the gas metallicity, presence of dust (the depletion of metals into dust grains), and possible deviations due to specific nucleosynthesis, for example, $\alpha$-element enhancements. We aim to derive the metallicities, dust depletion, and $\alpha$-element enhancements in the neutral ISM of gas-rich mostly-metal-poor distant galaxies (Damped Lyman-$\alpha$ absorbers, DLAs). Furthermore, we aim to constrain the distribution of $\alpha$-element enhancements with metallicity in these galaxies. We have constrained, for the first time, the distribution of the $\alpha$-element enhancement with metallicity in the neutral ISM in distant galaxies. Less massive galaxies show an $\alpha$-element knee at lower metallicities than more massive galaxies. This can be explained by a lower star formation rate in less massive galaxies. If this collective behaviour can be interpreted in the same way as it is for individual systems, this would suggest that more massive and metal-rich systems evolve to higher metallicities before the contribution of SN-Ia to [$\alpha$/Fe] levels out that of core-collapse SNe. This finding may plausibly be supported by different SFRs in galaxies of different masses. Overall, our results offer important clues to the study of chemical evolution in distant galaxies.Comment: 22 pages, 20 figures. Submitted to A&

Discrete radar ambiguity problems

In this paper, we pursue the study of the radar ambiguity problem started in \cite{Ja,GJP}. More precisely, for a given function $u$ we ask for all functions $v$ (called \emph{ambiguity partners}) such that the ambiguity functions of $u$ and $v$ have same modulus. In some cases, $v$ may be given by some elementary transformation of $u$ and is then called a \emph{trivial partner} of $u$ otherwise we call it a \emph{strange partner}. Our focus here is on two discrete versions of the problem. For the first one, we restrict the problem to functions $u$ of the Hermite class, $u=P(x)e^{-x^2/2}$, thus reducing it to an algebraic problem on polynomials. Up to some mild restriction satisfied by quasi-all and almost-all polynomials, we show that such a function has only trivial partners. The second discretization, restricting the problem to pulse type signals, reduces to a combinatorial problem on matrices of a special form. We then exploit this to obtain new examples of functions that have only trivial partners. In particular, we show that most pulse type signals have only trivial partners. Finally, we clarify the notion of \emph{trivial partner}, showing that most previous counterexamples are still trivial in some restricted sense

Antibacterial activity of a chitosan-PVA-Ag+-Tobermorite composite for periodontal repair

A polymer-mineral composite was prepared by solvent casting a mixture of chitosan, poly(vinyl alcohol), and Ag+-exchanged tobermorite in dilute acetic acid and characterised by scanning electron microscopy and Fourier transform infrared spectroscopy. The in vitro bioactivity of the CPTAg membrane was confirmed by the formation of hydroxyapatite on its surface in simulated body fluid. The alkaline dissolution products of the tobermorite lattice buffered the acidic breakdown products of the chitosan polymer and the presence of silver ions resulted in marked antimicrobial action against S. aureus, P. aeruginosa, and E. coli. The in vitro cytocompatibility of the CPTAg membrane was confirmed using MG63 osteosarcoma cells. The findings of this preliminary study have indicated that chitosan-poly(vinyl alcohol)-Ag+-tobermorite composites may be suitable materials for guided tissue regeneration applications

The Social context of motorcycle riding and the key determinants influencing rider behavior: A qualitative investigation

Objective: Given the increasing popularity of motorcycle riding and heightened risk of injury or death associated with being a rider, this study explored rider behaviour as a determinant of rider safety and, in particular, key beliefs and motivations which influence such behaviour. To enhance the effectiveness of future education and training interventions, it is important to understand riders’ own views about what influences how they ride. Specifically, this study sought to identify key determinants of riders’ behaviour in relation to the social context of riding including social and identity-related influences relating to the group (group norms and group identity) as well as the self (moral/personal norm and self-identity). ----- ----- Method: Qualitative research was undertaken via group discussions with motorcycle riders (n = 41). Results: The findings revealed that those in the group with which one rides represent an important source of social influence. Also, the motorcyclist (group) identity was associated with a range of beliefs, expectations, and behaviours considered to be normative. Exploration of the construct of personal norm revealed that riders were most cognizant of the “wrong things to do” when riding; among those issues raised was the importance of protective clothing (albeit for the protection of others and, in particular, pillion passengers). Finally, self-identity as a motorcyclist appeared to be important to a rider’s self-concept and was likely to influence their on-road behaviour. ----- ----- Conclusion: Overall, the insight provided by the current study may facilitate the development of interventions including rider training as well as public education and mass media messages. The findings suggest that these interventions should incorporate factors associated with the social nature of riding in order to best align it with some of the key beliefs and motivations underpinning riders’ on-road behaviours

The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation

The small ribosomal subunit protein Rps15/uS19 is involved in early nucleolar ribosome biogenesis and subsequent nuclear export of pre-40S particles to the cytoplasm. In addition, the C-terminal tail of Rps15 was suggested to play a role in mature ribosomes, namely during translation elongation. Here, we show that Rps15 not only functions in nucleolar ribosome assembly but also in cytoplasmic pre-40S maturation, which is indicated by a strong genetic interaction between Rps15 and the 40S assembly factor Ltv1. Specifically, mutations either in the globular or C-terminal domain of Rps15 when combined with the non-essential ltv1 null allele are lethal or display a strong growth defect. However, not only rps15 ltv1 double mutants but also single rps15 C-terminal deletion mutants exhibit an accumulation of the 20S pre-rRNA in the cytoplasm, indicative of a cytoplasmic pre-40S maturation defect. Since in pre-40S particles, the C-terminal tail of Rps15 is positioned between assembly factors Rio2 and Tsr1, we further tested whether Tsr1 is genetically linked to Rps15, which indeed could be demonstrated. Thus, the integrity of the Rps15 C-terminal tail plays an important role during late pre-40S maturation, perhaps in a quality control step to ensure that only 40S ribosomal subunits with functional Rps15 C-terminal tail can efficiently enter translation. As mutations in the C-terminal tail of human RPS15 have been observed in connection with chronic lymphocytic leukaemia, it is possible that apart from defects in translation, an impaired late pre-40S maturation step in the cytoplasm could also be a reason for this disease.Austrian science fund (FWF) P27996- B21, P28874-B21Ministerio de Ciencia e Innovación PID2019–103850-GB-I00Agencia Estatal de Investigación AEI/10.13039/ 501100011033Junta de Andalucía P20_00581, BIO-27

Understanding the nature of FRII optical nuclei: a new diagnostic plane for radio galaxies

We extend our study of the nuclei of 3CR FR II radio galaxies through HST optical images up to z=0.3. In the majority of them an unresolved nucleus (central compact core, CCC) is found. We analyze their position in the plane formed by the radio and optical nuclear luminosities in relation to their optical spectral properties. The broad-lined objects (BLO) have the brightest nuclei: they are present only at optical luminosities nu L_nu > 4 X 10^42 erg s^-1 which we suggest might represent a threshold in the radiative efficiency combined to a small range of black hole masses. About 40 % of the high and low excitation galaxies (HEG and LEG) show CCC which resemble those previously detected in FR I galaxies, in apparent contrast to the unification model. The equivalent width of the [OIII] emission line (with respect to the nuclear luminosity) reveals the nature of these nuclei, indicating that the nuclei of HEG are obscured to our line of sight and only scattered radiation is observed. This implies that the population of FR II is composed by objects with different nuclear properties, and only a fraction of them can be unified with quasars.Comment: 11 pages, 6 figures, in press on Astronomy & Astrophysics, minor changes have been mad