THE ANALYSIS OF THE VASCULAR FLORA FROM THE NATURAL PARK VÂNATORI – NEAMT

This article analyses the flora from The Natural Park Vânatori – Neamt, in the aspect of the participation of different life - form categoriers, floristic elements and ecological indices

From competition to cross border cooperation

The research goal is to present the importance of cross-border cooperation in 21st century Europe. Highlighting the fact that cross-border cooperation has become one of the objectives of EU cohesion policy in the periode between 2007-2013. To see the benefits of cross border cooperation we introduced in this paper a case study. I present Romania – Bulgaria Cross Border Cooperation Programme: purpose, objectives and expected results and I try to emphasize that the cooperation cross border objectiv has a very important role in administrative convergence and legislation approximation in EU. Research methods used were comparative analysis, historical and analytical method for collecting data needed for research from documents prepared by the European Commission (reports, guides, reviews)

CONTRIBUTIONS TO THE STUDY OF VEGETATION FROM THE NATURAL PARK VANATORI NEAMT

As a result of the researches carried out between 2005-2007 in The Natural Park Vanatori Neamt, we identified two association: Cytiso – Quercetum petraeae Paucă 1941 quercetosum dalechampii Chifu et al. 1995 and Corylo avellanae – Carpinetum quercetosum pedunculiflorae Chifu et Sârbu 2001. This article describes these associations by taking into consideration main aspects of chorology, ecology, physiognomy and floristic composition, biological forms, floristic elements, ecological indexes

Expression of matrix metalloproteinases (MMPs)−2/-7/-9/-14 and tissue inhibitors of MMPs (TIMPs)−1/-2 in bovine cutaneous fibropapillomas associated with BPV-2 infection

Introduction: Bovine papillomaviruses −1/−2 (BPVs) are small non-enveloped double-stranded DNA viruses able to infect the skin of bovids and equids, causing development of neoplastic lesions such as bovine cutaneous fibropapillomas and equine sarcoid. Matrix metalloproteinases (MMPs) are a group of zinc-dependent endopeptidases that degrade basal membrane and extracellularmatrix, whose function is essential in physiological processes such as tissue remodeling and wound healing. MMPs activity is finely regulated by a balancing with expression of tissue inhibitors of MMPs (TIMPs), a process that is impaired during tumour development. BPV infection is associated with upregulation of MMPs and /or their unbalancing with TIMPs, contributing to local invasion and impairment of extracellular matrix remodeling in equine sarcoid; however, studies regarding this topic in bovine fibropapillomas are lacking. Methods: The aim of this study was to perform an immunohistochemical and biochemical analysis on a panel of MMPs and TIMPs in BPV-2 positive bovine cutaneous fibropapillomas vs. normal skin samples. Results: Immunohistochemistry revealed a cytoplasmic expression of MMP- 2 (15/19), a cytoplasmic and perinuclear immunoreactivity of MMP-7 (19/19) and MMP-9 (19/19), along with a cytoplasmic and nuclear pattern of MMP-14 (16/19), accompanied by a cytoplasmic expression of TIMP-1 (14/19) and TIMP-2 (18/19) in tumour samples; western blotting revealed an overexpression ofMMP-2 (8/9),MMP-7 (9/9) andMMP-9 (9/9), and a decreased level of MMP-14 (9/9), TIMP-1 (9/9) and TIMP-2 (9/9) in tumour versus normal skin samples. Moreover, gelatine zymography confirmed the expression of pro-active MMP-2 (9/9) and MMP-9 (9/9) and, most importantly, indicated the presence and increased activity of their active forms (82 and 62 kDa, respectively) in tumour samples. Frontiers in Veterinary Science 01 frontiersin.org Daraban Bocaneti et al. 10.3389/fvets.2022.1063580 Discussion: This is the first study describing MMPs and TIMPs in bovine cutaneous fibropapillomas and our results suggest that their unbalanced expression in presence of BPV-2 may play a significant role in tumour development. A further analysis of supplementaryMMPs and TIMPs could bring new important insights into the papillomavirus induced tumours

THE PROTECTED SPECIES INCLUDED IN ROMANIAN RED LIST FROM THE NATURAL PARK VÂNĂTORI-NEAMŢ

This article intends to present to you the protected plants from the Natural Park Vânători Neam

The contribution of european funds to legislative harmonization, institutional approximation and administrative convergence in the Balkans

Implementation of EU legislation on european funds in the national public administration produces a legislative harmonization, between Union and Member State and between Member States, and an institutional approximation between state's structures. In this respect, the member state develop a national legislation, starting from the european laws witch establish only the guidelines needed to be respected by the state, in the process of coordination and management of funds.administrative convergence; national public administrations; cohesion policy; public authorities