Behavior and the Response of Cancer Cells on Anticancer Drug Treatment Monitored with Microelectrode Array

AbstractA cell-based impedance biosensor using microelectrode array has been developed for monitoring cellular activities of MCF-7 breast cancer cells and evaluating drug-induced apoptosis. Using this device, different activities of cells such as cell attachment, adhesion, and spreading are monitored by measuring impedance spectra and interpreting the data using an electrical equivalent circuit. In order to demonstrate pharmaceutical relevance, the cells were treated with 25μM of anti-cancer drug Cisplatin. It was found that cell spreading caused a significant increase of impedance magnitude in the frequency range between 10kHz and 100kHz during 23h of incubation, which is reversed after 24h treatment with Cisplatin. This reversal is attributed to cell apoptosis, which is confirmed by microscopic observation of the cells

Spillover of a tobamovirus from the Australian indigenous flora to invasive weeds

The tobamovirus yellow tailflower mild mottle virus (YTMMV) was previously reported in wild plants of Anthocercis species (family Solanaceae) and other solanaceous indigenous species growing in natural habitats in Western Australia. Here, we undertook a survey of two introduced solanaceous weeds, namely Solanum nigrum (black nightshade) and Physalis peruviana (cape gooseberry) in the Perth metropolitan area and surrounds to determine if YTMMV has spread naturally to these species. At a remnant natural bushland site where both solanaceous weeds and indigenous Anthocercis hosts grew adjacent to one another, a proportion of S. nigrum and P. peruviana plants were asymptomatically-infected with YTMMV, confirming spillover had occurred. Populations of S. nigrum also grow as weeds in parts of the city isolated from remnant bushland and indigenous sources of YTMMV, and some of these populations were also infected with YTMMV. Fruit was harvested from virus-infected wild S. nigrum plants and the seed germinated under controlled conditions. Up to 80% of resultant seedlings derived from infected parent plants were infected with YTMMV, confirming that the virus is vertically-transmitted in S. nigrum, and therefore infection appears to be self-sustaining in this species. This is the first report of spillover of YTMMV to exotic weeds, and of vertical transmission of this tobamovirus. We discuss the roles of vertical and horizontal transmission in this spillover event, and its implications for biosecurity

Understanding Malaria Persistence: A Mixed-Methods Study on the Effectiveness of Malaria Elimination Strategies in South-Central Vietnam

Despite the scale-up of vector control, diagnosis and treatment, and health information campaigns, malaria persists in the forested areas of South-Central Vietnam, home to ethnic minority populations. A mixed-methods study using an exploratory sequential design was conducted in 10 Ra-glai villages in Bac Ai district of Ninh Thuan province to examine which social factors limited the effectiveness of the national malaria elimination strategy in the local setting. Territorial arrangements and mobility were found to directly limit the effectiveness of indoor residual spraying and long-lasting insectidical treated nets (LLINs). Households (n=410) were resettled in the “new villages” by the government, where they received brick houses (87.1%) and sufficient LLINs (97.3%). However, 97.6% of households went back to their “old villages” to continue slash-and-burn agriculture. In the old village, 48.5% of households lived in open-structured plot huts and only 5.7% of them had sufficient LLIN coverage. Household representatives believed malaria could be cured with antimalarials (57.8%), but also perceived non-malarial medicines, rituals, and vitamin supplements to be effective against malaria. Household members (n = 1,957) used public health services for their most recent illness (62.9%), but also reported to buy low-cost medicines from the private sector to treat fevers and discomfort as these were perceived to be the most cost-effective treatment option for slash-and-burn farmers. The study shows the relevance of understanding social factors to improve the uptake of public health interventions and calls for contextually adapted strategies for malaria elimination in ethnic minority populations in Vietnam and similar settings

Numerical study on bending response of precast segmental concrete beams externally prestressed with FRP tendons

This study numerically investigates the bending response of dry key-jointed precast segmental concrete girders/beams (PSCBs) prestressed with external fiber reinforced polymer (FRP) tendons by using commercial finite element analysis (FEA) software Abaqus/CAE. The experimentally-validated model was used to conduct an intensive parametric analysis with a focus on the second-order effect. There has not been a similar numerical study of PSCBs with external FRP tendons in the published literature yet. The results showed that due to the rectilinear rigid-body bending shape, the behavior of PSCBs with external tendons was similar to that with internal tendons only if placing the deviators next to the opening joints. The second-order effect on the beam's behavior and the harping effect on the tendon stress at deviators became more obvious when the deviators were located away from the opening joints. Both the second-order and harping effect were proportionate to the beam's displacement. Therefore, using a high reinforcing index (ω) or a low span-to-depth ratio (L/dp) could mitigate the second-order and harping effect at the ultimate stage because the ultimate displacement of the beam decreased when increasing ω or reducing L/dp. Commonly-used CFRP tendons (Young's modulus Ep = 145 GPa) were found to be the optimum to replace steel tendons in PSCBs with external tendons because they offered the PSCBs similar strength and ductility compared to steel tendons. The use of high-modulus CFRP tendons (e.g. Ep = 200 GPa) improved the stiffness and strength of PSCBs but greatly reduced the beam's ductility. Lastly, the analytical analyses showed that the existing models yielded unconservative estimations of the effective depth (dpu) and stress (fpu) of external FRP tendons at the ultimate stage in PSCBs

Thermo-hydro-mechanical responses of the host rock in the context of geological nuclear waste disposal

Deep geological disposal facility has been considered as the most appropriated solution for the safe longterm management of high-level radioactive waste (HLW). Geologic disposal solution consists of isolating the radioactive waste from the biosphere. Argillaceous rock has been selected in several countries as host formation due to its favorable properties to isolate radionuclides and chemical contaminants (very low permeability, stable, high retention capacity, self-sealing, etc). Clays in their natural state is usually saturated. Disposal of the exothermic waste packages in the repository leads to an increase in temperature within the host rock, which induces the pore pressure build-up due to the difference in thermal expansion coefficients of the pore water and the solid skeleton. The excess pore pressure generally leads to a decrease in the effective stress and can provoke thermally hydraulic fracturing or shear failure. Therefore, understanding the thermo-hydro-mechanical (THM) responses of the saturated host rock due to the heat generated form waste packages is a key issue to assess the feasibility of the repository. This paper aims at presenting coupled THM constitutive equations for a saturated porous medium and its finite element (FEM) discretization and solution. The solution is validated against analytical solution and other numerical results from a benchmark within an international project. FEM program is then used to describe the THM behavior of the host rock around a HLW repository (i.e. near field responses). Sensitivity analysis were performed to evaluate effect of material anisotropy and hydraulic condition on the micro-tunnel wall

Ultra-sharp asymmetric Fano-like resonance spectrum on Si photonic platform

In this paper, we report the generation of an ultra-sharp asymmetric resonance spectrum through Fano-like interference. This generation is accomplished by weakly coupling a high-quality factor (Q factor) Fabry–Pérot (FP) cavity and a low-Q factor FP cavity through evanescent waves. The high-Q FP cavity is formed by Sagnac loop mirrors, whilst the low-Q one is built by partially transmitting Sagnac loop reflectors. The working principle has been analytically established and numerically modelled by using temporal coupled-mode-theory (CMT), and verified using a prototype device fabricated on the 340 nm silicon-on-insulator (SOI) platform, patterned by deep ultraviolet (DUV) lithography. Pronounced asymmetric resonances with slopes up to 0.77 dB/pm have been successfully measured, which, to the best of our knowledge, is higher than the results reported in state-of-the-art devices in on-chip integrated Si photonic studies. The established theoretical analysis method can provide excellent design guidelines for devices with Fano-like resonances. The design principle can be applied to ultra-sensitive sensing, ultra-high extinction ratio switching, and more applications

A unified convergence analysis for shuffling-type gradient methods

In this paper, we propose a unified convergence analysis for a class of generic shuffling-type gradient methods for solving finite-sum optimization problems. Our analysis works with any sampling without replacement strategy and covers many known variants such as randomized reshuffling, deterministic or randomized single permutation, and cyclic and incremental gradient schemes. We focus on two different settings: strongly convex and nonconvex problems, but also discuss the non-strongly convex case. Our main contribution consists of new non-asymptotic and asymptotic convergence rates for a wide class of shuffling-type gradient methods in both nonconvex and convex settings. We also study uniformly randomized shuffling variants with different learning rates and model assumptions. While our rate in the nonconvex case is new and significantly improved over existing works under standard assumptions, the rate on the strongly convex one matches the existing best-known rates prior to this paper up to a constant factor without imposing a bounded gradient condition. Finally, we empirically illustrate our theoretical results via two numerical examples: nonconvex logistic regression and neural network training examples. As byproducts, our results suggest some appropriate choices for diminishing learning rates in certain shuffling variants

Faktor Penguat Pada Peningkatan Kinerja Karyawan PT. Gading Murni Surabaya

The purpose of this study is to determine which factors as an amplifier to improve the performance of employees of PT. Gading Murni Surabaya, between the leadership style and compensation received by employees. This study uses a survey approach by collecting data using a questionnaire of 50 respondents then analyzed using quantitative methods. The regession equation is Y = 8.009 + 0,223 X1 + 0,240 X2. The results of this study concluded that the leadership style and compensation variables had a corrected item total correlation value exceeding r table = 0.284 and the reliability test of the leadership style variable, and the alpha cronbach's compensation results exceeded 0.060, which means that the variable was valid and reliable. Leadership and compensation styles also simultaneously have a significant effect on employee performance. And the independent variable that has the largest beta coefficient is the compensation variable (X2) with a beta coefficient of 0.240.   Keywords : Leadership style, Compesation and Employee Performance