Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions

Indexación: ScieloEucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.Financial support: This work was partially funded by Universidad Andrés Bello (DI Proyect: 04-05/1) and MIFAB (Proyect: P04-071-F) and by the Microsoft Joint Research Program

Evaluation of ADAS with a supported-driver model for desired allocation of tasks between human and technology performance

Partly automated driving is relevant for solving mobility problems, but also causes concerns with respect to the driver‟s reliability in task performance. The supported driver model presented in this paper is therefore intended to answer the question, what type of support and in which circumstances, will enhance the driver‟s ability to control the vehicle. It became apparent that prerequisites for performing tasks differ per driving task‟s type and require different support. The possible support for each driving task‟s type, has been combined with support-types to reduce the error causations from each different performance level (i.e. knowledge-based, rule-based and skill-based performance). The allocation of support in relation to performance level and driving task‟s type resulted in a supported driver model and this model relates the requested circumstances to appropriate support types. Among three tested ADAS systems, semi-automated parking showed best allocation of support; converting the demanding parallel parking task into a rather routine-like operation

Design of Experiments for Screening

The aim of this paper is to review methods of designing screening experiments, ranging from designs originally developed for physical experiments to those especially tailored to experiments on numerical models. The strengths and weaknesses of the various designs for screening variables in numerical models are discussed. First, classes of factorial designs for experiments to estimate main effects and interactions through a linear statistical model are described, specifically regular and nonregular fractional factorial designs, supersaturated designs and systematic fractional replicate designs. Generic issues of aliasing, bias and cancellation of factorial effects are discussed. Second, group screening experiments are considered including factorial group screening and sequential bifurcation. Third, random sampling plans are discussed including Latin hypercube sampling and sampling plans to estimate elementary effects. Fourth, a variety of modelling methods commonly employed with screening designs are briefly described. Finally, a novel study demonstrates six screening methods on two frequently-used exemplars, and their performances are compared

Velocity-space sensitivity of the time-of-flight neutron spectrometer at JET

The velocity-space sensitivities of fast-ion diagnostics are often described by so-called weight functions. Recently, we formulated weight functions showing the velocity-space sensitivity of the often dominant beam-target part of neutron energy spectra. These weight functions for neutron emission spectrometry (NES) are independent of the particular NES diagnostic. Here we apply these NES weight functions to the time-of-flight spectrometer TOFOR at JET. By taking the instrumental response function of TOFOR into account, we calculate time-of-flight NES weight functions that enable us to directly determine the velocity-space sensitivity of a given part of a measured time-of-flight spectrum from TOFOR

On the mechanisms governing gas penetration into a tokamak plasma during a massive gas injection

A new 1D radial fluid code, IMAGINE, is used to simulate the penetration of gas into a tokamak plasma during a massive gas injection (MGI). The main result is that the gas is in general strongly braked as it reaches the plasma, due to mechanisms related to charge exchange and (to a smaller extent) recombination. As a result, only a fraction of the gas penetrates into the plasma. Also, a shock wave is created in the gas which propagates away from the plasma, braking and compressing the incoming gas. Simulation results are quantitatively consistent, at least in terms of orders of magnitude, with experimental data for a D 2 MGI into a JET Ohmic plasma. Simulations of MGI into the background plasma surrounding a runaway electron beam show that if the background electron density is too high, the gas may not penetrate, suggesting a possible explanation for the recent results of Reux et al in JET (2015 Nucl. Fusion 55 093013)

Relationship of edge localized mode burst times with divertor flux loop signal phase in JET

A phase relationship is identified between sequential edge localized modes (ELMs) occurrence times in a set of H-mode tokamak plasmas to the voltage measured in full flux azimuthal loops in the divertor region. We focus on plasmas in the Joint European Torus where a steady H-mode is sustained over several seconds, during which ELMs are observed in the Be II emission at the divertor. The ELMs analysed arise from intrinsic ELMing, in that there is no deliberate intent to control the ELMing process by external means. We use ELM timings derived from the Be II signal to perform direct time domain analysis of the full flux loop VLD2 and VLD3 signals, which provide a high cadence global measurement proportional to the voltage induced by changes in poloidal magnetic flux. Specifically, we examine how the time interval between pairs of successive ELMs is linked to the time-evolving phase of the full flux loop signals. Each ELM produces a clear early pulse in the full flux loop signals, whose peak time is used to condition our analysis. The arrival time of the following ELM, relative to this pulse, is found to fall into one of two categories: (i) prompt ELMs, which are directly paced by the initial response seen in the flux loop signals; and (ii) all other ELMs, which occur after the initial response of the full flux loop signals has decayed in amplitude. The times at which ELMs in category (ii) occur, relative to the first ELM of the pair, are clustered at times when the instantaneous phase of the full flux loop signal is close to its value at the time of the first ELM

New large-bodied mammals from the Late Oligocene site of Chilga, Ethiopia

Newly recovered fossil proboscideans and embrithopods from Chilga, Ethiopia are described and evaluated taxonomically. They are dated to ca. 28–27 Ma (late Oligocene), temporally intermediate between late Eocene–early Oligocene Afro−Arabian faunas dominated by archaic, endemic taxa, and replacement faunas of the early Miocene marked by a massive influx of Eurasian migrants. The paucity of similar−aged sites in Afro−Arabia makes Chilga critical for delineating the initiation and sequence of this faunal turnover. While most of the genera present at Chilga persist from older Afro−Arabian localities, at higher elevation and farther inland than elsewhere, there are no Eurasian mammals in the fauna. However, the archaic endemics from Chilga differ morphometrically from their older congeners, and include a new embrithopod, Arsinoitherium giganteum sp. nov., and novel species of elephantiform proboscideans, Phiomia major sp. nov., aff. Palaeomastodon sp. nov. A, and aff. Palaeomastodon sp. nov. B. New, primitive deinotheres and gomphotheres also occur at Chilga, extending the fossil records of these proboscideans considerably back in time. The Chilga deinothere, Chilgatherium harrisi sp. nov., differs sufficiently from Prodeinotherium and Deinotherium to be placed in its own subfamily, Chilgatheriinae subfam. nov. The Chilga gomphothere is smaller than Miocene elephantoids, and is referred to cf. Gomphotherium sp. nov. Together, this evidence suggests that indigenous Afro−Arabian taxa had greater ecological versatility than previously suspected and continued to enjoy successful evolutionary trajectories into the late Paleogene. Thus, as they spread into Afro−Arabia, new immigrants from Eurasia may have encountered vibrant local mammalian communities. The demise of many endemic inhabitants followed and remains poorly understood