14 research outputs found
Empirical orthogonal function analyses of Tropical Atlantic sea surface temperature and wind stress : 1964-1979
Statistics of Atmospheric Correlations
For a large class of quantum systems the statistical properties of their
spectrum show remarkable agreement with random matrix predictions. Recent
advances show that the scope of random matrix theory is much wider. In this
work, we show that the random matrix approach can be beneficially applied to a
completely different classical domain, namely, to the empirical correlation
matrices obtained from the analysis of the basic atmospheric parameters that
characterise the state of atmosphere. We show that the spectrum of atmospheric
correlation matrices satisfy the random matrix prescription. In particular, the
eigenmodes of the atmospheric empirical correlation matrices that have physical
significance are marked by deviations from the eigenvector distribution.Comment: 8 pages, 9 figs, revtex; To appear in Phys. Rev.
Empirical orthogonal function analyses of Tropical Atlantic sea surface temperature and wind stress : 1964-1979
Dithiocarbamates are teratogenic to developing zebrafish through inhibition of lysyl oxidase activity
Dithiocarbamates (DTCs) are a class of compounds that are extensively used in agriculture as pesticides. As such, humans and wildlife are undoubtedly exposed to these chemicals. Although DTCs are thought to be relatively safe due to their short half lives, it is well established that they are teratogenic to vertebrates, especially to fish. In zebrafish, these teratogenic effects are characterized by distorted notochord development and shortened anterior to posterior axis. DTCs are known copper (Cu) chelators but this does not fully explain the observed teratogenic effects. We show here that DTCs cause malformations in zebrafish that highly resemble teratogenic effects observed by direct inhibition of a group of cuproenzymes termed lysyl oxidases (LOX). Additionally, we demonstrate that partial knockdown of three LOX genes, lox, loxl1 and loxl5b, sensitizes the developing embryo to DTC exposure. Finally, we show that DTCs directly inhibit zebrafish LOX activity in an ex vivo amine oxidase assay. Taken together, these results provide the first evidence that DTC induced teratogenic effects are, at least in part, caused by direct inhibition of LOX activity. Š 2009 Elsevier Inc
An investigation of the relationship between subâSaharan rainfall and global sea surface temperatures
Structure-function relationships of beta-D-glucan endo- and exohydrolases from higher plants
(1â3),(1â4)-β-D-Glucans represent an important component of cell walls in the Poaceae family of higher plants. A number of glycoside endo- and exohydrolases is required for the depolymerization of (1â3),(1â4)-β-Dglucans in germinated grain or for the partial hydrolysis of the polysaccharide in elongating vegetative tissues. The enzymes include (1â3),(1â4)-β-D-glucan endohydrolases (EC 3.2.1.73), which are classified as family 17 glycoside hydrolases, (1â4)-β-D-glucan glucohydrolases (family 1) and β-D-glucan exohydrolases (family 3). Kinetic analyses of hydrolytic reactions enable the definition of action patterns, the thermodynamics of substrate binding, and the construction of subsite maps. Mechanism-based inhibitors and substrate analogues have been used to study the spatial orientation of the substrate in the active sites of the enzymes, at the atomic level. The inhibitors and substrate analogues also allow us to define the catalytic mechanisms of the enzymes and to identify catalytic amino acid residues. Three-dimensional structures of (1â3),(1â4)-β-D-glucan endohydrolases, (1â4)- β-D-glucan glucohydrolases and β-D-glucan exohydrolases are available or can be reliably modelled from the crystal structures of related enzymes. Substrate analogues have been diffused into crystals for solving of the threedimensional structures of enzyme-substrate complexes. This information provides valuable insights into potential biological roles of the enzymes in the degradation of the barley (1â3),(1â4)-β-D-glucans during endosperm mobilization and in cell elongation.Maria Hrmova and Geoffrey B. Finche
Structure-function relationships of β-D-glucan endo- and exohydrolases from higher plants
Reprinted from Plant Molecular Biology, Volume 47 (1, 11),2001Maria Hrmova, Geoffrey B. Finche