New combined kinetic and thermodynamic approach to model glucose-6-phosphate dehydrogenase activity and stability

G6PD from S. cerevisiae was partitioned in this work by a reverse micellar system containing SL in isooctane and hexanol, using some significant variations with respect to the standard extraction procedure to improve the yield. The influence of the extraction process on the kinetic and thermodynamic properties of this enzyme was investigated, since these parameters can provide useful information about the activity of S. cerevisiae pentose phosphate pathway and the biosynthesis of several physiologically important biomolecules. To this purpose a new modeling approach combining kinetic and thermodynamic parameters of both enzyme activity and thermal inactivation was proposed, which gave more reliable results than previous attempts