Gene discovery and Pre-breeding in cereals for broad resistance against insects adaptable to variable environments

Climate change is expected to cause drastic changes in the incidence of disease and pests throughout the world, also leading to the occurrence of highly variable insect pests. One approach to minimize the losses in crop yields due to highly variable insects is the introgression of multiple resistance genes into adapted crop cultivars. The objectives of the study are to: 1) Identify wheat and barley germplasm from around the world that are resistant to various Russian wheat aphid biotypes in various countries, 2) develop genomic tools for marker-assisted breeding, and 3) introgress multiple resistance genes into more adapted wheat and barley cultivar. We have evaluated more than 80 wheat lines and over 200 barley lines from various parts of the world against a number of RWA biotypes from USA, Mexico, Hungary, France, Chile and Iran. Wheat and barley lines with moderate to good levels of resistance were identified. Genetic analysis of lines has revealed significant diversity. With the use of Doubled-haploid and F2:3 populations we have mapped resistance genes from various sources on chromosomes 1DS and 7DS in wheat, and 1H, 2H, 3H and 7H in barley. Closely linked SSR and Diversity array technology (DArT) markers have been identified, and these markers offer opportunities for marker-assisted breeding. The presentation will also outline the strategies for introgressing multiple resistance genes to adapted wheat and barley cultivars that could have better potential for minimizing yield loses due to increased incidence of insects because of climate change

Gene discovery and pre-breeding for Russian wheat aphid resistance in wheat

Pre-breeding research has a significant role in linking between genetic resources and breeding as it shortens the time frame between genetic enhancement and the development of new, improved crop varieties. The Russian wheat aphid (RWA), Diuraphis noxia, is one of the most damaging insect pests of wheat throughout the World. Although it is not yet present, its incursion to Australia is expected to cause substantial loses to the grains industry and therefore prebreeding research against this insect is seen as a high priority in Australia. The objectives of the study are to: 1) Identify available RWA resistant wheat germplasm from around the world and characterize against available RWA biotypes in various countries, 2) develop genomic tools for marker-assisted breeding, and 3) introgress new RWA resistance genes into Australian wheat and barley cultivars. We have evaluated a wide range of wheat lines from various parts of the world against a number of RWA biotypes from USA, Argentina, Mexico, Hungary, South Africa, France, Chile, Iran, and Syria. Wheat lines with moderate to good levels of resistance were identified. Genetic analysis of RWA resistant wheat lines has revealed significant diversity. To date, with the use of F2 and DH populations we have mapped genes for RWA resistance on chromosomes iDS and 7D. Closely linked SSR and DArT have been identified. These markers are also being used to identify wheat contigs potentially carrying the resistance genes. Introgression of resistance genes to adapted wheat lines have progressed up to BC4 generation. As we have identified some wheat lines that are resistant to both RWA and Ug99 in Kenya, crosses were also established to introgress resistance genes to both pests

Gene discovery and molecular Pre-breeding for insect resistance in wheat

No abstract availabl

Pre-emptive breeding and molecular marker development for resistance to Russian wheat aphid in barley and wheat: An international collaboration

No abstract availabl

A global effort in breeding for resistance to Russian wheat aphid in wheat and barley

Oral presentation - See attache

Gene discovery and molecular Pre-breeding for insect resistance in wheat and barley

Pre-breeding research has a significant role in linking between genetic resources and breeding as it shortens the time frame between genetic enhancement and the development of new, improved crop varieties. The Russian wheat aphid (RWA), Diuraphis noxia, is one of the most damaging insect pests of wheat and barley throughout the World. Although it is not yet present, its incursion to Australia is expected cause substantial loses to the grains industry and therefore pre-breeding research against this insect is seen as a high priority in Australia. The objectives of the study are to: 1) Identify available RWA resistant wheat and barley germplasm from around the world and characterize against available RWA biotypes in various countries, 2) develop genomic tools for marker-assisted breeding, and 3) introgress new RWA resistance genes into more adapted wheat and barley backgrounds. We have evaluated a wide range of wheat and barley lines from various parts of the world against a number of RWA biotypes from USA, Argentina, Mexico, Hungary, South Africa, France, Chile, Iran, and Syria. Wheat and barley lines with moderate to good levels of resistance were identified. Genetic analysis of RWA resistant wheat lines has revealed significant diversity. To date, with the use of F2 and DH populations we have mapped genes from four new sources of wheat for RWA resistance on chromosomes 1DS and 7D. Closely linked SSR and Diversity array technology (DArT) markers have been identified. These markers are also being used to identify wheat contigs potentially carrying the resistance genes. Introgression of resistance genes to adapted wheat and barley lines have progressed up to BC3 generation. As we have identified some wheat lines that are resistant to both RWA and Ug99 in Kenya, crosses were also established to introgress resistance genes to both pests

Safeguarding world wheat and barley production against Russian wheat aphid: An international germplasm evaluation and pre-breeding initiative

No abstract availabl

Pre-emptive breeding and molecular marker development for resistance to russian wheat aphid in wheat and barley

No abstract availabl