Interaction between Iron Oxide Nanoparticles and HepaRG Cells: A Preliminary In Vitro

Nowadays, the use of iron oxide nanoparticles is widespread to label cells for magnetic resonance imaging tracking. More recently, magnetic labeling provides promising new opportunities for tissue engineering by controlling and manipulating cells through the action of an external magnetic field. The present work describes nonspecific labeling of metabolically competent HepaRG hepatocytes with anionic iron oxide nanoparticles. An interaction was observed between nanoparticles and studied cells, which were easily attracted when exposed to a magnet. No cytotoxicity was detected in the hepatocytes after 24 hours of incubation with iron oxide nanoparticles. Impact on HepaRG metabolization activity was assessed. Although a slight decrease in the metabolite generation was observed after exposure to nanoparticles (2 mM in iron), the enzymatic capacity was maintained. These results pave the way for 3D cultures of magnetic labeled HepaRG cells by using a magnetic field

The biomass market as an ecological innovation

Celem artykułu jest podkreślenie konieczności rozwoju rynku biomasy dla produkcji biopaliw, ukazanie rozmiaru tego rynku, a także perspektyw jego rozwoju. Za główny czynnik rozwoju uznane zostały zintegrowane innowacje ekologiczne. Przedstawiono powiązania pomiędzy uczestnikami rynku biomasy oraz sposób powstawania i wdrażania innowacji.The aim of the article was to analyze the biomass market in Poland. It was found that biomass can be an important source of biofuel, which in turn can provide sources of energy for power plants and thermal power stations. Such sequence of energy production requires important innovations to be introduced in agriculture, chemical industry, as well as engineering and power industry

Rheological properties of superparamagnetic iron oxide nanoparticles

The present study focuses on the rheological properties of polyethylene glycol (PEG) modified, positively charged, and negatively charged superparamagnetic iron oxide nanoparticles (SPIONs) at different temperatures. We hypothesized that the surface properties of these nanoparticles in the water did not affect their rheological properties. These nanoparticles had not the same surface properties as SPIONs-PEG had not to charge on their surface whereas positively charged and negatively charged ones with amine and carboxyl groups as their surfaces had positive and negative surface charges, respectively. However, their rheological behaviors were not different from each other. The comparative rheological study of SPIONs revealed their pseudo-Newtonian behavior. The viscosity of SPIONs decreased with the increase in temperature. At low shear rates, the shear stress of SPIONs was independent of rate and increased with the increase of rate. Moreover, at high shear rates, the shear stress for PEG-SPIONs was more than those for positively charged and negatively charged SPIONs. These measurements also revealed that at high shear rates, the shear stress of samples decreased with the increase of temperature. The shear stress of samples decreased with the increase of shear strain and the temperature. We also observed that all the samples had the same amount of shear strain at each shear stress, which indicated the exact resistance of SPIONs to deformation. Furthermore, the shear modulus decreased with time for these nanoparticles. These results suggest that these nanoparticles are promising candidates with appropriate properties for fluid processing applications and drug vectors in biomedical applications

1,4-diarylpiperazines and analogs as anti-tubercular agents: synthesis and biological evaluation36446

&lt;p&gt;Despite progress in modern chemotherapy to combat tuberculosis, the causative pathogen&lt;/p&gt;</p

Toward a new and noninvasive diagnostic method of papillary thyroid cancer by using peptide vectorized contrast agents targeted to galectin-1

The incidence of papillary thyroid cancer has increased these last decades due to a better detection. High prevalence of nodules combined with the low incidence of thyroid cancers constitutes an important diagnostic challenge. We propose to develop an alternative diagnostic method to reduce the number of useless and painful thyroidectomies using a vectorized contrast agent for magnetic resonance imaging. Galectin-1 (gal-1), a protein overexpressed in well-differentiated thyroid cancer, has been targeted with a randomized linear 12-mer peptide library using the phage display technique. Selected peptides have been conjugated to ultrasmall superparamagnetic particles of iron oxide (USPIO). Peptides and their corresponding contrast agents have been tested in vitro for their specific binding and toxicity. Two peptides (P1 and P7) were selected according to their affinity toward gal-1. Their binding has been revealed by immunohistochemistry on human thyroid cancer biopsies, and they were co-localized with gal-1 by immunofluorescence on TPC-1 cell line. Both peptides induce a decrease in TPC-1 cells’ adhesion to gal-1 immobilized on culture plates. After coupling to USPIO, the peptides preserved their affinity toward gal-1. Their specific binding has been corroborated by co-localization with gal-1 expressed by TPC-1 cells and by their ability to compete with anti-gal-1 antibody. The peptides and their USPIO derivatives produce no toxicity in HepaRG cells as determined by MTT assay. The vectorized contrast agents are potential imaging probes for thyroid cancer diagnosis. Moreover, the two gal-1-targeted peptides prevent cancer cell adhesion by interacting with the carbohydrate-recognition domain of gal-1.SCOPUS: ar.jinfo:eu-repo/semantics/publishe