Kinetics of assembly and decay of complement components on E.coli 0111:B4. preparation of stables intermediates.

The preparation of bacterial intermediates bearing complement components at various steps of the complement sequence was investigated by suspending immunoglobulin M-opsonized Escherichia coli O111:B4 cells in complement-deficient sera at different temperatures and ionic strengths. The optimal conditions for the formation of the intermediates at Tmax were found to be an ionic strength of 0.091 mu and a temperature of 37 degrees C, except for BAC142, which could be formed equally well at room temperature. In contrast to all the other intermediates, which, once formed at Tmax, were stable in the presence of the whole serum, BAC142 decayed with a half-life of 10 min due to the lability of bound C2. Washing with a buffer of either 0.091 or 0.046 mu did not affect the bacterial intermediates, with the exception of BAC1-3 formed either in the presence of C5-deficient serum or with purified C3 added to BAC142. All the intermediates were found to be stable after incubation in 0.091-mu buffer for 30 min at 37 degrees C

A generic design platform for generic photonic foundries

A software platform able to fulfill the requirements of the new approach in photonic integration through the establishment of generic foundries is presented. The kernel is a circuit simulator able to combine the foundry building blocks described by accurate models to design and analyze complex circuits in the spectral domain

Statistical design in integrated optics

The 'design on tolerance' concept in integrated optic is introduced. The aim of this technique is to design a circuit that is robust to statistical variations of the parameters due to technological processes and aging. The numerical technique permits to design a generic circuit that maximizes the yield of the production process and at the same time locates the most critical parameters of the circuit

Distinct PMN bactericidal protein for E.coli 0111:B4 coated with either the fifth or the eight component of the complement system.

In Complement, Nov. 198

Photonics Integration: a Building Blocks Approach for a Generic Foundry Model

“Building block” and “circuit simulation” concepts are introduced and demonstrated in the optical domain similarly to electronic and microwave fields, allowing analysis, design and fast prototyping of complex integrated optical circuits

Statistical design in integrated optics

The 'design on tolerance' concept in integrated optic is introduced. The aim of this technique is to design a circuit that is robust to statistical variations of the parameters due to technological processes and aging. The numerical technique permits to design a generic circuit that maximizes the yield of the production process and at the same time locates the most critical parameters of the circuit

L\u2019ottavo componente del complemento (C8) come modulatore del killing di E.coli ad opera di proteine battericide di PMN umani.

Salsomaggiore (Parma

Validation of the building block based approach for the design of photonic integrated circuits

The concept of building block and circuit-model simulation in photonics are presented and discussed, pointing out their key role in a generic foundry approach. Circuit simulation based on building-block approach, in addition and alternative to the classical electromagnetic analysis, emerges as the most useful framework for a deep exploitation of the potentiality of photonics for the large scale integration of complex circuits. The reliability of building-block based design and simulation of photonics integrated circuits is discussed and comparisons between simulated and measured behaviour of three challenging test circuits are used as proof-of-concept of the validity of this approach

Bactericidal activities of human polymorphonuclear leukocyte proteins against E.coli 0111:B4 coated with C5 o C8.

The postnuclear supernatant of disrupted polymorphonuclear leukocytes exhibited bactericidal activity on Escherichia coli O111:B4 coated with immunoglobulin M antibodies and C5 or C8 but not on C3- or C7-coated bacteria. To characterize this antimicrobial activity further, granules obtained from the postnuclear supernatant were extracted with sodium acetate (pH 4) and the soluble extract was subsequently fractionated through carboxymethyl cellulose and Sephacryl S-200. Over 90% of the activity present in the starting material was recovered in the soluble granule extract. Kinetic and dose-response analyses of the bacterial activity of the polymorphonuclear leukocyte extract on BAC1-5 and BAC1-8 revealed different susceptibilities to killing of these two bacterial intermediates; they also differed for their susceptibilities to killing at 37 degrees C and at room temperature. The suggestion raised by these data, that BAC1-5 and BAC1-8 could be killed by different bactericidal factors, was confirmed by the findings that separate fractions of the soluble granule extract obtained by carboxymethyl cellulose and Sephacryl S-200 chromatography exhibited specific activity on either BAC1-5 or BAC1-8, whereas other fractions were active on both intermediates