Second-Generation Reforms in Infrastructure Services

During the past two decades, Latin American countries have made pioneering efforts in reforming infrastructure services. The "first generation of reforms" encompassed widespread privatization, deregulation and restructuring of the provision of energy, water, transport and telecommunications services. Second-Generation Reforms in Infrastructure Services evaluates the current challenges, leading to the consolidation of the initial reforms. This volume deals with post-privatization dispute settlement mechanisms, access arrangements in network industries, and inroads to effective competition in the reformed industries. The authors evaluate a set of contractual adjustments resulting from renegotiations and disputes that have taken place since the beginning of the reform process. In an effort to promote competition in the provision of public services, the authors suggest some practical rules for pricing access in network industries. The book presents a dynamic, global vision of second-generation reforms underway in energy markets around the world

Second-Generation Reforms in Infrastructure Services

During the past two decades, Latin American countries have made pioneering efforts in reforming infrastructure services. The "first generation of reforms" encompassed widespread privatization, deregulation and restructuring of the provision of energy, water, transport and telecommunications services. Second-Generation Reforms in Infrastructure Services evaluates the current challenges, leading to the consolidation of the initial reforms. This volume deals with post-privatization dispute settlement mechanisms, access arrangements in network industries, and inroads to effective competition in the reformed industries. The authors evaluate a set of contractual adjustments resulting from renegotiations and disputes that have taken place since the beginning of the reform process. In an effort to promote competition in the provision of public services, the authors suggest some practical rules for pricing access in network industries. The book presents a dynamic, global vision of second-generation reforms underway in energy markets around the world

Bell-like homeodomain selectively regulates the high-irradiance response of phytochrome A

Plant responses mediated by phytochrome A display a first phase saturated by transient light signals and a second phase requiring sustained excitation with far-red light (FR). These discrete outcomes, respectively so-called very-low-fluence response (VLFR) and high-irradiance response (HIR), are appropriate in different environmental and developmental contexts but the mechanisms that regulate the switch remain unexplored. Promoter analysis of a light-responsive target gene revealed a motif necessary for HIR but not for VLFR. This motif is required for binding of the Bell-like homeodomain 1 (BLH1) to the promoter in in vitro and in yeast 1-hybrid experiments. Promoter substitutions that increased BLH1 binding also enhanced HIR. blh1 mutants showed reduced responses to continuous FR and to deep canopy shadelight, but they retained normal responses to pulsed FR or red light and unfiltered sunlight. BLH1 enhanced BLH1 expression and its promotion by FR. We conclude that BLH1 specifically regulates HIR and not VLFR of phytochrome A

Physiological characterisation of acuB deletion in Aspergillus niger

The acuB gene of Aspergillus niger is an ortholog of facB in Aspergillus nidulans. Under carbon-repression conditions, facB is repressed, thereby preventing acetate metabolism when the repressing carbon source is present. Even though facB is reported to be repressed directly by CreA, it is believed that a basal level of FacB activity exists under glucose-repressive conditions. In the present study, the effect of deletion of acuB on the physiology of A. niger was assessed. Differences in organic acid and acetate production, enzyme activities and extracellular amino and non-amino organic acid production were determined under glucose-repressing and -derepressing conditions. Furthermore, consumption of alternative carbon sources (e.g. xylose, citrate, lactate and succinate) was investigated. It was shown that AcuB has pleiotropic effects on the physiology of A. niger. The results indicate that metabolic pathways that are not directly involved in acetate metabolism are influenced by acuB deletion. Clear differences in organic acid consumption and production were detected between the a dagger acuB and reference strain. However, the hypothesis that AcuB is responsible for basal AcuA activity necessary for activation of acetate metabolic pathways, even during growth on glucose, could not be confirmed. The experiments demonstrated that also when acuB was deleted, no acetate was formed. Therefore, AcuB cannot be the only activator of AcuA, and another control mechanism has to be available for activating AcuA