Analysis of the Movement of Chlamydomonas Flagella: The Function of the Radial-spoke System Is Revealed by Comparison of Wild-type and Mutant Flagella

The mutation uni-1 gives rise to uniflagellate Chlamydomonas cells which rotate around a fixed point in the microscope field, so that the flagellar bending pattern can be photographed easily . This has allowed us to make a detailed analysis of the wild-type flagellar bending pattern and the bending patterns of flagella on several mutant strains. Cells containing uni-1, and recombinants of uni-1 with the suppressor mutations, sup(_pf)-1 and sup(_pf)-3, show the typical asymmetric bending pattern associated with forward swimming in Chlamydomonas, although sup(_pf)-1 flagella have about one-half the normal beat frequency, apparently as the result of defective function of the outer dynein arms. The pf-17 mutation has been shown to produce nonmotile flagella in which radial spoke heads and five characteristic axonemal polypeptides are missing. Recombinants containing pf-17 and either sup(_pf)-1 or sup(_pf)-3 have motile flagella, but still lack radial-spoke heads and the associated polypeptides . The flagellar bending pattern of these recombinants lacking radial-spoke heads is a nearly symmetric, large amplitude pattern which is quite unlike the wild-type pattern . However, the presence of an intact radial-spoke system is not required to convert active sliding into bending and is not required for bend initiation and bend propagation, since all of these processes are active in the sup(_pf) pf-17 recombinants. The function of the radial-spoke system appears to be to convert the symmetric bending pattern displayed by these recombinants into the asymmetric bending pattern required for efficient swimming, by inhibiting the development of reverse bends during the recovery phase of the bending cycle

The Pulsation Mode and Distance of the Cepheid FF Aquilae

The determination of pulsation mode and distance for field Cepheids is a complicated problem best resolved by a luminosity estimate. For illustration a technique based on spectroscopic luminosity discrimination is applied to the 4.47d s-Cepheid FF Aql. Line ratios in high dispersion spectra of the variable yield values of =-3.40+-0.02 s.e.(+-0.04 s.d.), average effective temperature Teff=6195+-24 K, and intrinsic color (-)o = +0.506+-0.007, corresponding to a reddening of E(B-V)=0.25+-0.01, or E(B-V)(B0)=0.26+-0.01. The skewed light curve, intrinsic color, and luminosity of FF Aql are consistent with fundamental mode pulsation for a small amplitude classical Cepheid on the blue side of the instability strip, not a sinusoidal pulsator. A distance of 413+-14 pc is estimated from the Cepheid's angular diameter in conjunction with a mean radius of =39.0+-0.7 Rsun inferred from its luminosity and effective temperature. The dust extinction towards FF Aql is described by a ratio of total-to-selective extinction of Rv=Av/E(B-V)=3.16+-0.34 according to the star's apparent distance modulus.Comment: To appear in ApJ

The ART of IAM: The Winning Strategy for the 2006 Competition

In many dynamic open systems, agents have to interact with one another to achieve their goals. Here, agents may be self-interested, and when trusted to perform an action for others, may betray that trust by not performing the actions as required. In addition, due to the size of such systems, agents will often interact with other agents with which they have little or no past experience. This situation has led to the development of a number of trust and reputation models, which aim to facilitate an agent's decision making in the face of uncertainty regarding the behaviour of its peers. However, these multifarious models employ a variety of different representations of trust between agents, and measure performance in many different ways. This has made it hard to adequately evaluate the relative properties of different models, raising the need for a common platform on which to compare competing mechanisms. To this end, the ART Testbed Competition has been proposed, in which agents using different trust models compete against each other to provide services in an open marketplace. In this paper, we present the winning strategy for this competition in 2006, provide an analysis of the factors that led to this success, and discuss lessons learnt from the competition about issues of trust in multiagent systems in general. Our strategy, IAM, is Intelligent (using statistical models for opponent modelling), Abstemious (spending its money parsimoniously based on its trust model) and Moral (providing fair and honest feedback to those that request it)

Congenital Cytomegalovirus Infection: A Narrative Review of the Issues in Screening and Management From a Panel of European Experts.

Maternal primary and non-primary cytomegalovirus (CMV) infection during pregnancy can result in in utero transmission to the developing fetus. Congenital CMV (cCMV) can result in significant morbidity, mortality or long-term sequelae, including sensorineural hearing loss, the most common sequela. As a leading cause of congenital infections worldwide, cCMV infection meets many of the criteria for screening. However, currently there are no universal programs that offer maternal or neonatal screening to identify infected mothers and infants, no vaccines to prevent infection, and no efficacious and safe therapies available for the treatment of maternal or fetal CMV infection. Data has shown that there are several maternal and neonatal screening strategies, and diagnostic methodologies, that allow the identification of those at risk of developing sequelae and adequately detect cCMV. Nevertheless, many questions remain unanswered in this field. Well-designed clinical trials to address several facets of CMV treatment (in pregnant women, CMV-infected fetuses and both symptomatic and asymptomatic neonates and children) are required. Prevention (vaccines), biology and transmission factors associated with non-primary CMV, and the cost-effectiveness of universal screening, all demand further exploration to fully realize the ultimate goal of preventing cCMV. In the meantime, prevention of primary infection during pregnancy should be championed to all by means of hygiene education

Dynamics at the angle of repose: jamming, bistability, and collapse

When a sandpile relaxes under vibration, it is known that its measured angle of repose is bistable in a range of values bounded by a material-dependent maximal angle of stability; thus, at the same angle of repose, a sandpile can be stationary or avalanching, depending on its history. In the nearly jammed slow dynamical regime, sandpile collapse to a zero angle of repose can also occur, as a rare event. We claim here that fluctuations of {\it dilatancy} (or local density) are the key ingredient that can explain such varied phenomena. In this work, we model the dynamics of the angle of repose and of the density fluctuations, in the presence of external noise, by means of coupled stochastic equations. Among other things, we are able to describe sandpile collapse in terms of an activated process, where an effective temperature (related to the density as well as to the external vibration intensity) competes against the configurational barriers created by the density fluctuations.Comment: 15 pages, 1 figure. Minor changes and update

Spectral properties of zero temperature dynamics in a model of a compacting granular column

The compacting of a column of grains has been studied using a one-dimensional Ising model with long range directed interactions in which down and up spins represent orientations of the grain having or not having an associated void. When the column is not shaken (zero 'temperature') the motion becomes highly constrained and under most circumstances we find that the generator of the stochastic dynamics assumes an unusual form: many eigenvalues become degenerate, but the associated multi-dimensional invariant spaces have but a single eigenvector. There is no spectral expansion and a Jordan form must be used. Many properties of the dynamics are established here analytically; some are not. General issues associated with the Jordan form are also taken up.Comment: 34 pages, 4 figures, 3 table