A Quantitative Study of Bone Marrow Grafting: Implications for Human Bone Marrow Infusion

FOLLOWING the researches of Jacobson and his colleagues (Jacobson et al., 1948, 1949a, 1949b, 1950) Lorenz showed that the intravenous infusion of viable isologous marrow cells prevented the death of lethally irradiated mice and guinea pigs (Lorenz et al., 1951). Protection against lethal doses of some radiomimetic drugs has also been effected in this way (Weston et al., 1957; Sartorelli an

The fundamental cycle of concept construction underlying various theoretical frameworks

In this paper, the development of mathematical concepts over time is considered. Particular reference is given to the shifting of attention from step-by-step procedures that are performed in time, to symbolism that can be manipulated as mental entities on paper and in the mind. The development is analysed using different theoretical perspectives, including the SOLO model and various theories of concept construction to reveal a fundamental cycle underlying the building of concepts that features widely in different ways of thinking that occurs throughout mathematical learning

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

BACKGROUND: Eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins in Eimeria species has opened up the possibility of using Eimeria live vaccines to deliver heterologous antigens and function as multivalent vaccine vectors that could protect chickens against a range of pathogens. RESULTS: In this study, genetic complementation was used to express immunoprotective virus antigens in Eimeria tenella. Infectious bursal disease virus (IBDV) causes Gumboro, an immunosuppressive disease that affects productivity and can interfere with the efficacy of poultry vaccination programmes. Infectious laryngotracheitis virus (ILTV) causes a highly transmissible respiratory disease for which strong cellular immunity and antibody responses are required for effective vaccination. Genes encoding the VP2 protein from a very virulent strain of IBDV (vvVP2) and glycoprotein I from ILTV (gI) were cloned downstream of 5’Et-Actin or 5’Et-TIF promoter regions in plasmids that also contained a mCitrine fluorescent reporter cassette under control of the 5’Et-MIC1 promoter. The plasmids were introduced by nucleofection into E. tenella sporozoites, which were then used to infect chickens. Progeny oocysts were sorted by FACS and passaged several times in vivo until the proportion of fluorescent parasites in each transgenic population reached ~20 % and the number of transgene copies per parasite genome decreased to < 10. All populations were found to transcribe and express the transgene and induced the generation of low titre, transgene-specific antibodies when used to immunise chickens. CONCLUSIONS: E. tenella can express antigens of other poultry pathogens that are successfully recognised by the chicken immune system. Nonetheless, further work has to be done in order to improve the levels of expression for its future use as a multivalent vaccine vector. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1756-2) contains supplementary material, which is available to authorized users

On the Spectrum of Field Quadratures for a Finite Number of Photons

The spectrum and eigenstates of any field quadrature operator restricted to a finite number $N$ of photons are studied, in terms of the Hermite polynomials. By (naturally) defining \textit{approximate} eigenstates, which represent highly localized wavefunctions with up to $N$ photons, one can arrive at an appropriate notion of limit for the spectrum of the quadrature as $N$ goes to infinity, in the sense that the limit coincides with the spectrum of the infinite-dimensional quadrature operator. In particular, this notion allows the spectra of truncated phase operators to tend to the complete unit circle, as one would expect. A regular structure for the zeros of the Christoffel-Darboux kernel is also shown.Comment: 16 pages, 11 figure

Creation of macroscopic quantum superposition states by a measurement

We propose a novel protocol for the creation of macroscopic quantum superposition (MQS) states based on a measurement of a non-monotonous function of a quantum collective variable. The main advantage of this protocol is that it does not require switching on and off nonlinear interactions in the system. We predict this protocol to allow the creation of multiatom MQS by measuring the number of atoms coherently outcoupled from a two-component (spinor) Bose-Einstein condensate.Comment: 4 pages (revtex4), 2 figure

Large-uncertainty intelligent states for angular momentum and angle

The equality in the uncertainty principle for linear momentum and position is obtained for states which also minimize the uncertainty product. However, in the uncertainty relation for angular momentum and angular position both sides of the inequality are state dependent and therefore the intelligent states, which satisfy the equality, do not necessarily give a minimum for the uncertainty product. In this paper, we highlight the difference between intelligent states and minimum uncertainty states by investigating a class of intelligent states which obey the equality in the angular uncertainty relation while having an arbitrarily large uncertainty product. To develop an understanding for the uncertainties of angle and angular momentum for the large-uncertainty intelligent states we compare exact solutions with analytical approximations in two limiting cases.Comment: 20 pages, 9 figures, submitted to J. Opt. B special issue in connection with ICSSUR 2005 conferenc

Constraints for quantum logic arising from conservation laws and field fluctuations

We explore the connections between the constraints on the precision of quantum logical operations that arise from a conservation law, and those arising from quantum field fluctuations. We show that the conservation-law based constraints apply in a number of situations of experimental interest, such as Raman excitations, and atoms in free space interacting with the multimode vacuum. We also show that for these systems, and for states with a sufficiently large photon number, the conservation-law based constraint represents an ultimate limit closely related to the fluctuations in the quantum field phase.Comment: To appear in J. Opt. B: Quantum Semiclass. Opt., special issue on quantum contro