The possibility of hyperbaric oxygenation in the correction of the secretory impaired fertility in men

The infertility problem during a marriage affects demographic indicators significantly and has not only medical and biological but important social significance as well. In Russia, about 20 % of married couples remain infertile. With that, in half of cases this is caused with pathological changes in male reproductive system. Until now, a comprehensive andrologic examination is not capable of finding out the reason of pathospermia in 40–72 % of cases of male infertility and, as a result, of assigning the appropriate etiotropic treatment. A comparative dynamic study of spermograms of 107 patients with idiopathic pathozoospermia depending on various types of treatment has been held immediately, 1 month, and 2 months after completion of the therapy course. It has been determined that significantly greater and sustained improvement in quality and quantity of ejaculate is achieved in 1 month as compared to monotherapy with L-carnitine in combination with hyperbaric oxygenation by eliminating the effects of hypoxia and oxidativestress, and the effect is maintained during the whole term of observation

ЗНАЧИМОСТЬ ОПРЕДЕЛЕНИЯ ИНТЕРЛЕЙКИНА-8 ДЛЯ ДИФФЕРЕНЦИАЛЬНОЙ ДИАГНОСТИКИ ПИЕЛОНЕФРИТА И ЦИСТИТА У ЖЕНЩИН ПРИ НЕФРОЛИТИАЗЕ

The objective was to optimize the topical diagnosis of the inflammatory process in the kidney and bladder in women with urolithiasisMethods and materials. Examined were 31 women aged 25 to 65 y. o., hospitalized for exacerbation of chronic cystitis with urolithiasis. The comparison group consisted of 15 healthy people without a history of urological diseases. In urine taken  separately from the bladder and ureter, the level of pro-inflammatory interleukin 8 (IL 8) was determined by enzyme-linked immunosorbent assay.Results. With an isolated exacerbation of chronic cystitis in the urine taken from the ureters, the concentration of IL 8 ((39±7.6) pg/ml) did not go beyond the normal indicators ((35±7) pg/ml), while the content of this cytokine in the bladder exceeded the norm by at least 3 times ((120±3.5) pg/ml). With a combination of exacerbations of inflammation in the bladder and kidneys, the concentration of IL 8 in the bladder was about 10 times higher than the control values ((350±27) pg/ml), and in the ureter from the affected side, no less than 8 times ((280±16) pg/ml).Conclusions. A comparison of the content of IL 8 in urine from organs (kidneys, bladder) that are supposedly affected by the inflammatory process makes it possible to clarify the localization of the focus of inflammation in the urinary system.Цель. Оптимизация топической диагностики воспалительного процесса в почке и мочевом пузыре у женщин при мочекаменной болезни.Методы и материалы. Обследована 31 женщина в возрасте от 25 до 65 лет, которые были госпитализированы по поводу обострения хронического цистита на фоне мочекаменной болезни. Группу сравнения сформировали 15 здоровых людей без урологических заболеваний в анамнезе. В моче, раздельно взятой из мочевого пузыря и мочеточника, методом иммуноферментного анализа определяли уровень провоспалительного интерлейкина-8 (ИЛ-8).Результаты. При изолированном обострении хронического цистита в моче, полученной из  мочеточников, концентрация ИЛ-8 ((39±7,6) пг/мл) не выходила за пределы нормативных  показателей ((35±7) пг/мл), в то время как содержание этого цитокина в мочевом пузыре превышало норму не менее чем в 3 раза ((120±3,5) пг/мл). При сочетании обострений воспаления в мочевом пузыре и в почках концентрация ИЛ-8 в мочевом пузыре возрастала примерно в 10 раз относительно контрольных значений ((350±27) пг/мл), а в мочеточнике с пораженной стороны – не менее чем в 8 раз ((280±16) пг/мл).Выводы. Таким образом, сравнение содержания ИЛ-8 в моче из предположительно пораженных воспалительным процессом органов (почек, мочевого пузыря) дает возможность уточнить локализацию очага воспаления в мочевыделительной системе. 

THE IMPORTANCE OF DETERMINING INTERLEUKIN-8 FOR THE DIFFERENTIAL DIAGNOSIS OF PYELONEPHRITIS AND CYSTITIS IN WOMEN WITH NEPHROLITHIASIS

The objective was to optimize the topical diagnosis of the inflammatory process in the kidney and bladder in women with urolithiasisMethods and materials. Examined were 31 women aged 25 to 65 y. o., hospitalized for exacerbation of chronic cystitis with urolithiasis. The comparison group consisted of 15 healthy people without a history of urological diseases. In urine taken  separately from the bladder and ureter, the level of pro-inflammatory interleukin 8 (IL 8) was determined by enzyme-linked immunosorbent assay.Results. With an isolated exacerbation of chronic cystitis in the urine taken from the ureters, the concentration of IL 8 ((39±7.6) pg/ml) did not go beyond the normal indicators ((35±7) pg/ml), while the content of this cytokine in the bladder exceeded the norm by at least 3 times ((120±3.5) pg/ml). With a combination of exacerbations of inflammation in the bladder and kidneys, the concentration of IL 8 in the bladder was about 10 times higher than the control values ((350±27) pg/ml), and in the ureter from the affected side, no less than 8 times ((280±16) pg/ml).Conclusions. A comparison of the content of IL 8 in urine from organs (kidneys, bladder) that are supposedly affected by the inflammatory process makes it possible to clarify the localization of the focus of inflammation in the urinary system

AVIDITY EVALUATION OF LOCAL IgA ANTIBODIES IN PERSONS IMMUNIZED WITH LIVE INFLUENZA VACCINE

Abstract. At present, immunogenicity evaluation of influenza vaccines is performed by quantitative assessment of increased serum antibodies. It was, however, shown that the degree of human defense against influenza is mostly related to their qualitative characteristics, i.e., avidity (functional activity). Leading role of local immunity is demonstrated in protection against influenza. Such immunity is mediated by IgA antibodies from mucosal airways. Meanwhile, the avidity issues for local antibodies still remain open.In present study, an attempt was undertaken to evaluate post-vaccination local immunological memory for influenza A virus, according to IgA antibodies from upper respiratory secretions. Two techniques were used to evaluate antibody avidity, that were previously applied for studying this phenomenon with serum imunoglobulins, i.e., a dynamic test (measurement of antigen-antibody reaction rates), and a test with urea, a chaotropic agent (avidity is determined as a strength of antigen-antibody complex). A total of 202 persons (18 to 20 years old) were enrolled into the study.With both tests, a broad range of individual avidity values was observed for the antibodies. A significant cohort (up to 30 per cent) of persons immunized with live influenza vaccine, showed sharply increased avidity of secretory IgA antibodies by both methods, along with accumulation of these immunoglobulins after vaccination. A reverse relationship is revealed between avidity levels of these antibodies before vaccination, and increase of this parameter post-immunization. The data present convincing arguments for specific renewal of local humoral immunological memory, as induced by live influenza vaccine. The study substantiates a necessity for application of the both tests in parallel, when determining avidity of secretory IgA antibodies. (Med. Immunol., vol. 10, N 4-5, pp 423-430)

The possibility of hyperbaric oxygenation in the correction of the secretory impaired fertility in men

The infertility problem during a marriage affects demographic indicators significantly and has not only medical and biological but important social significance as well. In Russia, about 20 % of married couples remain infertile. With that, in half of cases this is caused with pathological changes in male reproductive system. Until now, a comprehensive andrologic examination is not capable of finding out the reason of pathospermia in 40–72 % of cases of male infertility and, as a result, of assigning the appropriate etiotropic treatment. A comparative dynamic study of spermograms of 107 patients with idiopathic pathozoospermia depending on various types of treatment has been held immediately, 1 month, and 2 months after completion of the therapy course. It has been determined that significantly greater and sustained improvement in quality and quantity of ejaculate is achieved in 1 month as compared to monotherapy with L-carnitine in combination with hyperbaric oxygenation by eliminating the effects of hypoxia and oxidativestress, and the effect is maintained during the whole term of observation.</p

EVALUATION OF T CELL IMMUNOLOGICAL MEMORY BY MEANS OF CD45+ EXPRESSION IN HUMANS VACCINATED WITH LIVE REASSORTANT INFLUENZA VACCINE

Abstract. A full-scale development of post-vaccinal immunity is determined by T and B cell-dependent immunological memory which is formed in response to vaccine injection. However, a capacity of existing and newly developed vaccines to stimulate T cell-dependent immunological memory is very poorly studied. Present work is a first attempt of analyzing this issue, with respect to effects of live mucosal influenza vaccine.The study involved fifty-seven healthy young persons, in whom percentage of T cells with СD45RO+, CD45RA+, CD4+СD45RO+, CD4+CD45RA+, CD8+СD45RO+, and CD8+CD45RA+ phenotypes was determined pre- and post-vaccination.Following vaccination, increased average T cell levels of all the mentioned phenotypes was revealed in those volunteers who responded to the vaccine with elevated serum antibody titers. Individual data analysis has shown that, among individuals with antibody response, a percentage of persons with CD45RO+ T cells was 35 to 57%, as compared to vaccinated persons without such antibody response (11 to 22%, p &lt; 0.05; p &lt; 0.01). When using CD45RA+ marker, appropriate values among group 1 volunteers varied between 14 and 36%, whereas for group 2, only one case was detected with expression with significantly increased CD8+CD45RA+, expression on T cells post-vaccination.Hence, a single immunization with live influenza vaccine is followed by significantly increased levels of peripheral CTLs (CD8+) and Th (СD4+) with СD45R0+ (memory cells) and СD45RА+ (naive) phenotypes in sufficient cohort of immunized persons (35 to 60%). Certain relations exist between post-vaccinal accumulation of memory T cells in peripheral blood, and development of a systemic humoral response to vaccination. However, this connection is not absolute, since an increase in these cells post-vaccination occurs in some persons without such a response (up to 25% of immunized individuals). (Med. Immunol., vol. 10, N 6, pp 535-542)

Diagnosis and treatment of erectile dysfunction in patients with benign prostatic hyperplasia

The efficacy and safety of the Russian phosphodiesterase type 5 inhibitor sildenafil-C3 in combination with a natural complex of the Russian multicomponent dietary supplement (DS) Erectogenon were investigated in 75 patients with erectile dysfunction (ED) and benign prostatic hyperplasia (BPH). Clinical and Doppler ultrasound examinations revealed moderate ED in 34 study group patients who had received combined treatment with sildenafil and the DS Erectogenon for 4 weeks. In the same perod, 21 patients from a comparative group (Group 2) had taken sildenafil only and 20 patients from a comparative group (Group 3) had used the natural biocomplex DS Erectogenon only. The investigation revealed mild ED in the patients from Groups 2 and 3. The immediate and late treatment results were assessed 4 and 12 weeks after the end of treatment, respectively. Combined therapy with sildenafil + Erectogenon was found to statistically significantly enhance its efficiency and to improve quality of sexual life in 85.3 % of the study group patients who had moderate ED. This patient group had a more marked positive clinical effect (a mean increase in erection quality by 7.0 scores by the International Index of Erectile Function, a rise in penile blood flow by 3.5 times, and a reduction in the International Prostatitis Symptom Index by 5 scores). Sildenafiil-C3 or Erectogenon monotherapy provided a lower effect in the comparative groups. The findings confirm that combined therapy with the Russian drugs sildenafil-C3 and DS Erectogenon is effective, safe, and well-compatible and causes no adverse reactions and the synergism of the components of the biocomplex DS Erectogenon prolongs the effect of daily low-dose sildenafil. The combined treatment may be recommended for the treatment of ED in patients with BPH, for this affects the pathogenesis of the disease, alleviates the progression of ED, and improves their quality of life. The treatment should be used in moderate ED and moderate reductions in urodynamic symptoms, by keeping in mind penile and prostatic Doppler ultrasound findings