6 research outputs found
Cytokine status in posttraumatic synovitis on the background of systemic and intra-articular use of NSAID and ozone
Joint damage initiates aseptic self-sustaining inflammation, which contributes the progression of post-traumatic destruction of tissues not only in the pathological focus, but also outside it, significantly expanding the zone of degenerative changes due to secondary alterations. One of the leading roles in pathogenesis of the inflammation belongs to secreted mediators-cytokines – that impart to the cells the proinflammatory potential and promote the long-term inflammation. These effects lead to disorganization of extracellular matrix and progressive disintegration of cartilage. In this regard, the development and implementation of new pathogenetic treatment methods of post-traumatic synovitis permits to limit the area of secondary alterations and activate reparative mechanisms in the lesion from the early terms, thus potentially improving the results of rehabilitation treatment and increasing efficiency of conventional therapy in post-traumatic synovitis. Numerous experimental and clinical studies have proven the effectiveness and safety of ozone therapy, e.g., in degenerative joint diseases. Despite extensive data highlighting effectiveness of ozone therapy in articular pathology, the study of cytokine profile when using this treatment of posttraumatic synovitis was performed only in few works, thus emphasizing the prospects for further research in this direction. The study was aimed for investigation of cytokine status in the patients with posttraumatic synovitis subjected to intravenous and intraarticular ozone therapy in combination with intra-articular administration of xefocam.The work is based on the results of examination and treatment of 69 patients with traumatic injuries of the knee joint, complicated by development of post-traumatic synovitis. Two study groups were formed, comparable in volume and type of joint injury. The patients from group I (35 cases) received conventional combined treatment. Among the mandatory measures, evacuation of a synovial-hemorrhagic punctate was performed from the cavity of damaged joint. Conservative therapy included NSAIDs, medications that improve microcirculation, at standard dosages, as well as physical therapy. In group II (34 patients), traditional therapy was supplemented with a 10-day course of intravenous injectable ozone therapy with 200 ml of NaCl solution at a concentration of 2.0 mg/l daily and intra-articular ozone injection at a concentration of 5 mg/l in a volume of 20 ml 5 times in a day. During arthroscopy, lavage of the joint cavity was performed with ozonated saline solution at a concentration of 2.0 mg/l. The ozone therapy was combined with three intra-articular injections of xefocam at a dose of 8 mg, once every 4 days. A patent for the invention was obtained for this treatment technology (No. 2456988 of 27.07.12). The cytokine profile was evaluated by the content of Pro-inflammatory (TNFα, IL-1β, IL-6, IL-17), regulatory (IL-2), Il-1β receptor antagonist, and anti-inflammatory (IL-4, IL-10) cytokines by solid-phase enzyme immunoassay with an indicator label in the form of peroxidase. Statistical analysis of the results was carried out using the Student criterion. Combined therapy of intravenous and intraarticular ozone therapy in combination with intra-articular injections of xefocam contributed to the inhibition of the inflammatory response, which is reflected in the dynamics of depression of the studied cytokines: simultaneous reduction of proinflammatory cytokines with the limitation of the growth of anti-inflammatory mediators. The final measurements showed a decrease in the content of proinflammatory cytokines: TNFα by 24.6% (p2 < 0.001); IL-17, by 17.3% (p2 < 0.01); IL-6, by 20.1% (p2 < 0.001); IL-1β, by 19.1% (p2 < 0.001), with a decrease in regulatory IL-2 by 25.7% (p2 < 0.001) and anti-inflammatory cytokines IL–10, by 21.3% (p2 < 0.001); Il – 4, by 25.7% (p2 < 0.001); IL-1ra, by 24.4% (p2 < 0.001), when compared to the data obtained with conventional treatment. The results obtained allow us to evaluate this method as highly effective in the treatment of post-traumatic synovitis, thus contributing to suppression of inflammatory response and reduces the secondary alteration of joint tissue structures, preventing the progression of post-traumatic osteoarthritis
ANTITUMOR EFFICACY OF LIPOSOMAL DOXORUBICIN HYDROCHLORIDE IN COMBINATION WITH TAMOXIFEN. EXPERIMENTAL STUDY
In this paper we evaluated the effectiveness of adjuvant chemotherapy in animals (n=30), which created a model of cancerogenesis. We created 3 equal studies: the 1st group control (n=10) - animals were monitored without treatment; the 2nd group (n=10) - animals received adjuvant therapy with a combination of drugs: liposomal doxorubicin hydrochloride+tamoxifen;the 3rd group (n=10) - animals received adjuvant therapy in a combination of doxorubicin hydrochloride (non-liposomal)+tamoxifen. All animals were monitored for a total of 30 days. As a result, the volume of the tumor in the group of animals treated with liposomal doxorubicin hydrochloride was almost 3 times less than in the control group, and 2 times less than in the rats treated with simple doxorubicin. We also recorded a significantly lower number of lung metastases in animals of the second group compared to other groups. CONCLUSION. Treatment of Walker 256 tumors with liposomal doxorubicin showed better efficacy and safety compared to non-liposomal doxorubicin
Dental status of patients with squamous cell carcinoma of the oropharyngeal region
Purpose of the study. To evaluate changes in the oral cavity in patients with squamous cell carcinoma of the oropharyngeal region after radiation therapy.Materials and methods. The study included 70 patients with oropharyngeal squamous cell carcinoma. All patients underwent external beam radiation therapy. We used dose fractionation mode: 5 fractions per week, single focal dose — 2G. The severity of oral mucositis was assessed using the RTOG evaluation scale.Results. Patients with carcinoma of the oropharyngeal region before the start of the main treatment had pathology of hard tooth tissues (CPU-14.62±1.08), gingival inflammation (PMA-41.71±4.98), periodontal inflammation (CPITN-2.54±0.62). After the end of radiation therapy, oral mucositis was detected in all patients (100%). We found changes in the negative side for all indicators of dental status indices compared to primary CPUs-15.26±0.90; CPITN-3.36±0.58; PMA-49.75±5.28 (p<0.05). Clinical signs of oral mucositis begin to appear as early as 7 days after the start of radiation therapy, RTOG=1,5. After 3 weeks, patients develop severe mucosal lesions with fibrinous plaque and severe pain syndrome (RTOG=3.0), requiring intensive drug therapy (p<0.05).Conclusion. In connection with the early appearance of post-radiation oral mucositis, preventive and therapeutic measures to improve the regeneration
EVALUATION OF THE CORTICOSTEROID RECEPTORS' LEVEL IN THE KIDNEYS OF RATS AFTER SYSTEMIC ISCHEMIA-REPERFUSION
BACKGROUND: With disturbance of the systemic blood supply, the body experiences hypoxia and stress. Under stress of any etiology, there is a non-specific rearrangement ofphysiological and biochemical processes. These processes occur under the influence of corticosteroid hormones. AIM: To determine the level of corticosteroid receptors in the kidneys of rats at different times after systemic ischemia-reperfusion. METHODS: The study included 80 male white rats. All the animals were divided into 2 groups. A model of systemic ischemia-reperfusion was created in the main group (n=70). Further, on 1, 3, 5, 7, 14, 21 and for 35 days, we determined the level of gluco - and mineralocorticoid receptors in the kidneys. RESULTS: In the animals of the main group, we observed a short-term period (during the first 3 days) of a decrease in the content of both glucorticoid (p<0.05) and mineralocorticoid (p<0.01) receptors. The dynamics of recovery of the level of corticosteroid receptors was 3 times faster than that of mineralocorticoid receptors. CONCLUSIONS: The dynamics of corticosteroid receptors' level in the kidneys of rats after ischemia caused by an arrest of systemic circulation show the recovery time after ischemia-reperfusion injury, which ensures the stability of an individual to hypoxia
CORRECTION OF THROMBOCYTOPOIESIS IN RATS WITH WALKER-256 CARCINOMA USING AN ANTIOXIDANT SUPPLEMENT IN THE SETTING OF CYTOSTATICS
In this study, we've evaluated the myelotoxic effect of treating Walker-256 carcinoma with cytostatics, followed by correction of thrombocytopenia with liposomal and liposome-free mexidol. The study included 60 rats, which, depending on the type of treatment, were divided into 5 groups. We recorded the greatest increase in the number of platelets on the background of liposomal mexidol both on the 3rd and 7th day after the start of chemotherapy. At the end of the monitoring, the myeloprotective effect was 30% higher in the liposomal mexidol compared to its non-liposomal form. CONCLUSION: Activation of thrombocytopoiesis on the background of cytostatic therapy helps to reduce the complications related to the use of chemotherapy
Radiological and Morphometric Features of Canalis Sinuosus in Russian Population: Cone-Beam Computed Tomography Study
Introduction. Cone-beam computed tomography (CBCT) is considered to be the most informative radiographic method for pre- and postoperative analysis of the maxillary anatomy and for avoiding further complication. Canalis sinuosus is one of such structures that damage can go along with bleeding and neurological symptomatology. The aim of the study was to investigate radiological and morphometric features of the canalis sinuosus in Russian population using CBCT technique. Materials and Methods. 150 CBCT scans of 61 males and 89 females aged from 24 to 80 years were retrospectively studied with different slice thickness and evaluated with regards to prevalence and diameter among age and gender groups in Russia. Results. CS prevalence in this study was 67%, and CS was most frequently presented in the lateral incisor region (33.5%). Women showed statistically higher CS prevalence (p<0.01) than the male group, and there was no statistically significant difference observed between occurrence and localization of CS and age groups. Conclusion. CBCT examination demonstrated good diagnostic efficiency in CS visualization, and the CS may have variations on its location and prevalence with statistically significant differences between the gender group and without significant differences among age groups and can depend on the population. © 2019 Avanesov Anatoly et al