Rollenspiel

Das Rollenspiel als Erhebungsmethode in der qualitativen Sozialforschung bietet, verbunden mit dem Auswertungsverfahren der tiefenhermeneutischen Textinterpretation, die Möglichkeit, kollektiv unbewusste Prozesse in Gruppen in ihrem Bedeutungsgehalt für die den Interaktionen zugrunde liegenden Muster zu erkennen und zu verstehen. Gruppendynamische Prozesse werden erfasst und das rollenspezifische Handeln der Gruppenteilnehmenden bezogen auf das jeweilige Erfahrungsfeld analysiert. In dem Beitrag werden die Durchführung der Rollenspiele sowie deren Dokumentation und die Auswertung der in den Rollenspielen erhobenen Daten vorgestellt, insbesondere anhand konkreter Anwendungsbeispiele die Dokumentations- und Auswertungsschritte praxisorientiert dargelegt und Limitationen der Einsatzes von Rollenspielen diskutiert

Common Sense, Traditional Structures and Higher Education: Reengineering as Fundamental Inquiry Abstract

This paper was presented at CUMREC ’98, The College and University Computer Users Association Conference. It is the intellectual property of the author(s). Permission to print out or disseminate all or part of this material is granted provided that the copies are not made or distributed for commercial advantage and that the title and authors of the paper appear. To copy or disseminate otherwise, or to republish in any form, requires written permission from the authors

polishCLR: a Nextflow workflow for polishing PacBio CLR genome assemblies

Long-read sequencing has revolutionized genome assembly, yielding highly contiguous, chromosome-level contigs. However, assemblies from some third generation long read technologies, such as Pacific Biosciences (PacBio) Continuous Long Reads (CLR), have a high error rate. Such errors can be corrected with short reads through a process called polishing. Although best practices for polishing non-model de novo genome assemblies were recently described by the Vertebrate Genome Project (VGP) Assembly community, there is a need for a publicly available, reproducible workflow that can be easily implemented and run on a conventional high performance computing environment. Here, we describe polishCLR (https://github.com/isugifNF/polishCLR), a reproducible Nextflow workflow that implements best practices for polishing assemblies made from CLR data. PolishCLR can be initiated from several input options that extend best practices to suboptimal cases. It also provides re-entry points throughout several key processes including identifying duplicate haplotypes in purge_dups, allowing a break for scaffolding if data are available, and throughout multiple rounds of polishing and evaluation with Arrow and FreeBayes. PolishCLR is containerized and publicly available for the greater assembly community as a tool to complete assemblies from existing, error-prone long-read data.This is a pre-print of the article Chang, Jennifer, Amanda R. Stahlke, Sivanandan Chudalayandi, Benjamin D. Rosen, Anna K. Childers, and Andrew Severin. "polishCLR: a Nextflow workflow for polishing PacBio CLR genome assemblies." bioRxiv (2022). DOI: 10.1101/2022.02.10.480011. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted

Population genomic structure in Goodman's mouse lemur reveals long‐standing separation of Madagascar's Central Highlands and eastern rainforests

Duke Lemur Centre publication #1510International audienceMadagascar's Central Highlands are largely composed of grasslands, interspersed with patches of forest. The historical perspective was that Madagascar's grasslands had anthropogenic origins, but emerging evidence suggests that grasslands were a component of the pre-human Central Highlands vegetation. Consequently, there is now vigorous debate regarding the extent to which these grasslands have expanded due to anthropogenic pressures. Here, we shed light on the temporal dynamics of Madagascar's vegetative composition by conducting a population genomic investigation of Goodman's mouse lemur (Microcebus lehilahytsara; Cheirogaleidae). These small-bodied primates occur both in Madagascar's eastern rainforests and in the Central Highlands, making them a valuable indicator species. Population divergences among forest-dwelling mammals will reflect changes to their habitat, including fragmentation, whereas patterns of post-divergence gene flow can reveal formerly wooded migration corridors. To explore these patterns, we used RADseq data to infer population genetic structure, demographic models of post-divergence gene flow, and population size change through time. The results offer evidence that open habitats are an ancient component of the Central Highlands, and that widespread forest fragmentation occurred naturally during a period of decreased precipitation near the last glacial maximum. Models of gene flow suggest that migration across the Central Highlands has been possible from the Pleistocene through the recent Holocene via riparian corridors. Though our findings support the hypothesis that Central Highland grasslands predate human arrival, we also find evidence for human-mediated population declines. This highlights the extent to which species imminently threatened by human-mediated deforestation may already be vulnerable from paleoclimatic conditions