An automatic method for the evaluation of xenobiotic toxicity on haematopoietic progenitors

International audienc

Effect of Ochratoxin A on human haematopoietic progenitors proliferation and differentiation: an in vitro study.

International audienceOchratoxin A (OTA) is a mycotoxin food and feed-contaminant known to induce nephro and hepatotoxicity in human and animal, and related to human Balkan Endemic Nephropathy. However, haematological troubles are also observed in case of acute OTA intoxication. These disorders observed in animals emphasise the necessity to determine if OTA exposure induce damage to haematopoietic system in human. The effect on haematopoiesis has been evaluated using in vitro clonogenic assays of the three lineages i.e., platelet, red and white blood cell progenitors. Human erythroblastic and granulomonocytic progenitor proliferation is decreased in the presence of 10(2) microM OTA. Platelet progenitors were destroyed at 10(2) microM OTA. For the lowest concentrations haematopoietic progenitor proliferation is not affected by OTA. Comparison with other mycotoxins known to be myelotoxic shows that OTA is less myelotoxic than trichothecenes

In vitro effects of diacetoxyscirpenol (DAS) on human and rat granulo-monocytic progenitors.

International audienceDiacetoxyscirpenol (DAS) is a trichothecene mycotoxin produced by various species of fungi. Trichothecenes are known as major contaminants of cereals and cereal-containing foods. DAS has been detected in agricultural products worldwide and persists in products after processing. In human as well as in animals, DAS consumption has been shown to induce haematological disorders (neutropenia, aplastic anemia). Granulo-monocytic progenitors (CFU-GM) from human umbilical cord blood and rat bone marrow have been cultured in the presence of DAS (from 10(-8) M to 5 x 10(-10) M) for 14 days. Study of concentration and effect relationships have shown a sharp effect of DAS on rat CFU-GM between 10(-7) M and 10(-8) M, while human CFU-GM are able to grow in the presence of 10(-8) M of the toxin. IC50 values on day 14 are respectively, 7.6 x 10(-9) M for human CFU-GM and 6.2 x 10(-9) M for rat CFU-GM

T-2 toxin inhibits the differentiation of human monocytes into dendritic cells and macrophages

International audienc

Evaluation of shellfish consumption in Nha Trang City, Southern coastal vietnam

International audienc

Evaluation of shellfish consumption in Nha Trang City, Southern coastal vietnam

International audienc

Exposure to Dishwashing Liquid Assessed in University Students from Brest City: A Preliminary Study-A First Approach to Household Products Exposure in France

International audienc

Response of human cord blood cells to styrene exposure: evaluation of its effects on apoptosis and gene expression by genomic technology

Styrene is one of the most important monomers produced worldwide, and it finds major use in the production of polystyrene, acrylonitrile-butadiene- styrene resins and unsaturated polystyrene resins. Epidemiological studies on styrene showed that the malignancies observed most frequently in humans after exposure are related to the lymphatic and haemopoietic system. IARC classified styrene a possible carcinogenic to humans (Group 2B). In this study, we evaluated the effect of styrene on gene expression profiles of human cord blood cells, as well as its activity on the apoptosis and bcl-2 related protein expression. Data demonstrated that, after 24 and 48 h of exposure, styrene (800 \u3bcM) induced an increase in the necrosis of mononuclear cord blood cells, whereas it did not cause any increase in the apoptotic process. Western blot analysis revealed a modified expression of Bax, BCl-2, c-Jun, c-Fos and Raf-1 proteins in the human cord blood cells after direct exposure to styrene, whereas p53 expression did not change. Furthermore, Macroarray analysis showed that styrene changed cord blood gene expression, inducing up-regulation of monocyte chemotactic protein 1 (MCP-1), and down-regulation of CC chemokine receptor type 1 (CCR-1) and SLP-76 tyrosine-phosphoprotein