69 research outputs found
Photodynamic therapy augments the efficacy of oncolytic vaccinia virus against primary and metastatic tumours in mice
in mic
Expression and Functional Studies of Ubiquitin C-Terminal Hydrolase L1 Regulated Genes
Deubiquitinating enzymes (DUBs) have been increasingly implicated in regulation of cellular processes, but a functional role for Ubiquitin C-terminal Hydrolases (UCHs), which has been largely relegated to processing of small ubiquitinated peptides, remains unexplored. One member of the UCH family, UCH L1, is expressed in a number of malignancies suggesting that this DUB might be involved in oncogenic processes, and increased expression and activity of UCH L1 have been detected in EBV-immortalized cell lines. Here we present an analysis of genes regulated by UCH L1 shown by microarray profiles obtained from cells in which expression of the gene was inhibited by RNAi. Microarray data were verified with subsequent real-time PCR analysis. We found that inhibition of UCH L1 activates genes that control apoptosis, cell cycle arrest and at the same time suppresses expression of genes involved in proliferation and migration pathways. These findings are complemented by biological assays for apoptosis, cell cycle progression and migration that support the data obtained from microarray analysis, and suggest that the multi-functional molecule UCH L1 plays a role in regulating principal pathways involved in oncogenesis
Xenohybridization of Epstein-Barr Virus-Transformed Cells for the Production of Human Monoclonal Antibodies
Human Anti-Tetanus Toxoid Monoclonal Antibody Secreted by EBV-Transformed Human B Cells Fused with Murine Myeloma
Cell Biology of Human IgM-Producing Hybridomas Derived from a Fusion of Human Spleen Lymphocytes with Mouse Myeloma Cells
Anti-bacterial antibodies in Epstein-Barr virus (EBV)-transformed oligoclonal B-cell lines established from normal persons and autoimmune disease patients
Expression of members of immunoglobulin gene family in somatic cell hybrids between human B and T cells.
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