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Not AvailableIn the current study, partial cDNA clones of inositol tetraphosphate 1-kinase (ITPK1) and inositol 1,4,5-trisphosphate kinase/inositol polyphosphate multikinase (IPK2), were isolated from embryo using RT-PCR and designated as AhITPK1 and AhIPK2 isoforms of the gene. The partial cDNA sequence of AhITPK1 and AhIPK2 genes have an open reading frame (ORF) of 1146 and 891bp respectively and showed high similarity to other plant genes. AhITPK1 shared high homology with Aradu. Q95MC of Arachis duranensis, had a single exon with no introns and belonged to ATP-grasp family of proteins. AhIPK2 shared high similarity with Aradu.24V9G of A. duranensis and contained three exons with 5’ and 3’ UTR’s on either side. Unlike other IPK2 genes, AhIPK2 possessed conserved domains such as PxxxDxKxG and [L/M][I/V]D[F/L][A/G][H/K]. Phylogenetic analysis grouped AhITPK1 with A. duranensis, A. ipinensis and Oryza brachyantha into one cluster, whereas AhIPK2 was grouped along with Cucumis melo and C. sativus. Evolutionarily, AhITPK1 and AhIPK2 were genetically distinct from other plant genera. Furthermore, real-time PCR analysis revealed high expression of AhITPK1 and AhIPK2 genes in the peanut embryo and flower bud. For the first time AhITPK1 (KR778986) and AhIPK2 (KR778988) genes belonging to phytic acid pathway from Arachis hypogaea were identified and characterized the expression pattern of these two isoforms on different tissues. These genes were found to be abundant in flower bud and embryo. Results suggest that embryo development significantly influences the expression of the two AhIPK isoforms in peanut. Evolutionarily they were found to be distinct from their parental species. This study is an important step toward understanding the role of these two AhIPK isoforms in phytic acid synthesis. However, future research involving RNAi-based functional characterization is warranted to establish their link to embryo development in peanut.United States Department of Agriculture (USDA) under Norman E. Borlaug International Agricultural Science and Technology Fellowship Program (Borlaug Fellowship Program) 201

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Not AvailableBreeding for low phytate peanut genotypes promises to be cost-effective intervention in the fight against micronutrient deficiencies in developing economies. However, tools and genomic resources are still not available to develop such varieties. Our study has identified genes in the phytic acid biosynthetic pathway that can be used to alter their expression, or identify genotypes that have lower expression of these genes that can be used in molecular breeding to reduce the phytate content in peanuts.Not Availabl

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Not AvailableBreeding for low phytate peanut genotypes promises to be cost-effective intervention in the fight against micronutrient deficiencies in developing economies. However, tools and genomic resources are still not available to develop such varieties.Our study has identified genes in the phytic acid biosynthetic pathway that can be used to alter their expression, or identify genotypes that have lower expression of these genes that can be used in molecular breeding to reduce the phytate content in peanuts.Not Availabl