219 research outputs found
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Ultraviolet protection on a snowball Earth
Habitats in the Antarctic provide an insight into habitats available on snowball earth. Physical UV protection on snowball earth would have been dominated by the manifestations of ice and snow in different habitats. The snowball period was a golden age of UV protection
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Shock recovery experiments confirm the possibility of transferring viable microorganisms from Mars to Earth
Extract from introduction: With regard to the impact and ejection phase we tested the case for the transfer of microorganisms from Mars to Earth. Using a high explosive set-up thin layers of bacterial endospores of Bacillus subtilis, of the lichen Xanthoria elegans and of the cyanobacterium Chroococcidiopsis sp. embedded between two plates of gabbro were subjected to 10, 20, 30, 40 and 50 GPa which is the pressure range observed in Martian meteorites [1]
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Life after shock: the mission from Mars to Earth
Extract from introduction: The minerals of the Martian meteorites collected so far indicate an exposure to shock waves in the pressure range of 5 to 55 GPa [1]. As terrestrial rocks are frequently inhabited by microbial communities, rocks ejected from a planet by impact processes may carry with them endolithic microorganisms, if microbial life existed/exists on this planet
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Impact experiments in support of “Lithopanspermia”: The route from Mars to Earth
Shock recovery experiments on a Martian analogue rock (gabbro) loaded with three types of microorganisms reveal that these organisms survive the impact and ejection phase on Mars at shock pressures up to about 50 GPa with exponentially decreasing survival rates
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The influence of shock pressure, pre-shock temperature, and host rock composition on the survival rate of endolithic microorganisms during impact ejection from Mars
Petrographic and biological analysis of shock recovery experiments confirms the possible life transport due to an impact from Mars to Earth
Panspermia, Past and Present: Astrophysical and Biophysical Conditions for the Dissemination of Life in Space
Astronomically, there are viable mechanisms for distributing organic material
throughout the Milky Way. Biologically, the destructive effects of ultraviolet
light and cosmic rays means that the majority of organisms arrive broken and
dead on a new world. The likelihood of conventional forms of panspermia must
therefore be considered low. However, the information content of dam-aged
biological molecules might serve to seed new life (necropanspermia).Comment: Accepted for publication in Space Science Review
Single-cell analysis reveals individual spore responses to simulated space vacuum
Outer space is a challenging environment for all forms of life, and dormant spores of bacteria have been frequently used to study the survival of terrestrial life in a space journey. Previous work showed that outer space vacuum alone can kill bacterial spores. However, the responses and mechanisms of resistance of individual spores to space vacuum are unclear. Here, we examined spores’ molecular changes under simulated space vacuum (~10−5 Pa) using micro-Raman spectroscopy and found that this vacuum did not cause significant denaturation of spore protein. Then, live-cell microscopy was developed to investigate the temporal events during germination, outgrowth, and growth of individual Bacillus spores. The results showed that after exposure to simulated space vacuum for 10 days, viability of spores of two Bacillus species was reduced up to 35%, but all spores retained their large Ca2 +-dipicolinic acid depot. Some of the killed spores did not germinate, and the remaining germinated but did not proceed to vegetative growth. The vacuum treatment slowed spore germination, and changed average times of all major germination events. In addition, viable vacuum-treated spores exhibited much greater sensitivity than untreated spores to dry heat and hyperosmotic stress. Among spores’ resistance mechanisms to high vacuum, DNA-protective α/β−type small acid-soluble proteins, and non- homologous end joining and base excision repair of DNA played the most important roles, especially against multiple cycles of vacuum treatment. Overall, these results give new insight into individual spore’s responses to space vacuum and provide new techniques for microorganism analysis at the single-cell level
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Needle Sampling Dates for Nutrient Evaluation in Christmas Tree Production
Christmas tree needle sampling to evaluate nutrient need is an established practice. Data to support the recommended fall sampling time in Oregon and Washington was not found. In addition, the recommendation for needle sampling of Fraser fir in North Carolina was made without data from winter months. The goal for this paper is to affirm or modify recommended needle sampling time. Needles of Douglas-fir, Turkish fir, Nordmann fir, Noble fir, Grand fir, and Fraser fir Christmas trees were collected monthly for a year, dried, and analyzed for N, P, K, Ca, Mg, and B. No single period was found for any species when needle nutrient concentration was stable for all elements investigated. Revised recommended needle collection times were chosen by integrating cultural practices, likelihood of nutrient deficiency, and needle nutrient concentration changes for species grown in an area. Needle collection is recommended during
February in western Oregon and Washington. The current sampling time, fall, is logical for Fraser fir Christmas tree production in North Carolina.Keywords: Noble fir (Abies procera),
Nordmann fir (Abies nordmanniana),
Grand fir (Abies grandis),
Tissue sampling,
Fraser fir (Abies fraseri),
Christmas trees,
Annual nutrient concentration,
Turkish fir (Abies bornmuelleriana),
needle sampling,
Douglas-fir (Pseudotsuga menziesii
Resistance of Antarctic black fungi and cryptoendolithic communities to simulated space and Martian conditions
Dried colonies of the Antarctic rock-inhabiting meristematic fungi
Cryomyces antarcticus CCFEE 515, CCFEE 534 and C. minteri
CCFEE 5187, as well as fragments of rocks colonized by the Antarctic
cryptoendolithic community, were exposed to a set of ground-based experiment
verification tests (EVTs) at the German Aerospace Center (DLR, Köln,
Germany). These were carried out to test the tolerance of these organisms in
view of their possible exposure to space conditions outside of the
International Space Station (ISS). Tests included single or combined simulated
space and Martian conditions. Responses were analysed both by cultural and
microscopic methods. Thereby, colony formation capacities were measured and
the cellular viability was assessed using live/dead dyes FUN 1 and SYTOX
Green. The results clearly suggest a general good resistance of all the
samples investigated. C. minteri CCFEE 5187, C. antarcticus
CCFEE 515 and colonized rocks were selected as suitable candidates to
withstand space flight and long-term permanence in space on the ISS in the
framework of the LIchens and Fungi Experiments (LIFE programme, European Space
Agency)
Analysis of miRNA and mRNA Expression Profiles Highlights Alterations in Ionizing Radiation Response of Human Lymphocytes under Modeled Microgravity
BACKGROUND: Ionizing radiation (IR) can be extremely harmful for human cells since an improper DNA-damage response (DDR) to IR can contribute to carcinogenesis initiation. Perturbations in DDR pathway can originate from alteration in the functionality of the microRNA-mediated gene regulation, being microRNAs (miRNAs) small noncoding RNA that act as post-transcriptional regulators of gene expression. In this study we gained insight into the role of miRNAs in the regulation of DDR to IR under microgravity, a condition of weightlessness experienced by astronauts during space missions, which could have a synergistic action on cells, increasing the risk of radiation exposure.
METHODOLOGY/PRINCIPAL FINDINGS: We analyzed miRNA expression profile of human peripheral blood lymphocytes (PBL) incubated for 4 and 24 h in normal gravity (1 g) and in modeled microgravity (MMG) during the repair time after irradiation with 0.2 and 2Gy of \u3b3-rays. Our results show that MMG alters miRNA expression signature of irradiated PBL by decreasing the number of radio-responsive miRNAs. Moreover, let-7i*, miR-7, miR-7-1*, miR-27a, miR-144, miR-200a, miR-598, miR-650 are deregulated by the combined action of radiation and MMG. Integrated analyses of miRNA and mRNA expression profiles, carried out on PBL of the same donors, identified significant miRNA-mRNA anti-correlations of DDR pathway. Gene Ontology analysis reports that the biological category of "Response to DNA damage" is enriched when PBL are incubated in 1 g but not in MMG. Moreover, some anti-correlated genes of p53-pathway show a different expression level between 1 g and MMG. Functional validation assays using luciferase reporter constructs confirmed miRNA-mRNA interactions derived from target prediction analyses.
CONCLUSIONS/SIGNIFICANCE: On the whole, by integrating the transcriptome and microRNome, we provide evidence that modeled microgravity can affects the DNA-damage response to IR in human PBL
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