Twin-Photon Confocal Microscopy

A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express {\bf 18}, 9765 (2010)] via the use of photon correlations. Here we achieve similar results in a different manner, introducing a triple-confocal correlated microscope which exploits the correlations present in optical parametric amplifiers. It is based on tight focusing of pump radiation onto a thin sample positioned in front of a nonlinear crystal, followed by coincidence detection of signal and idler photons, each focused onto a pinhole. This approach offers further resolution enhancement in confocal microscopy

Non Local Theories: New Rules for Old Diagrams

We show that a general variant of the Wick theorems can be used to reduce the time ordered products in the Gell-Mann & Low formula for a certain class on non local quantum field theories, including the case where the interaction Lagrangian is defined in terms of twisted products. The only necessary modification is the replacement of the Stueckelberg-Feynman propagator by the general propagator (the ``contractor'' of Denk and Schweda) D(y-y';tau-tau')= - i (Delta_+(y-y')theta(tau-tau')+Delta_+(y'-y)theta(tau'-tau)), where the violations of locality and causality are represented by the dependence of tau,tau' on other points, besides those involved in the contraction. This leads naturally to a diagrammatic expansion of the Gell-Mann & Low formula, in terms of the same diagrams as in the local case, the only necessary modification concerning the Feynman rules. The ordinary local theory is easily recovered as a special case, and there is a one-to-one correspondence between the local and non local contributions corresponding to the same diagrams, which is preserved while performing the large scale limit of the theory.Comment: LaTeX, 14 pages, 1 figure. Uses hyperref. Symmetry factors added; minor changes in the expositio

t(7;9)(q11.2;p13.2) PAX5/AUTS2

Short Communication on t(7;9)(q11.2;p13.2) PAX5/AUTS2, with data on clinics, and the genes implicated

Applications of Information Theory to Analysis of Neural Data

Information theory is a practical and theoretical framework developed for the study of communication over noisy channels. Its probabilistic basis and capacity to relate statistical structure to function make it ideally suited for studying information flow in the nervous system. It has a number of useful properties: it is a general measure sensitive to any relationship, not only linear effects; it has meaningful units which in many cases allow direct comparison between different experiments; and it can be used to study how much information can be gained by observing neural responses in single trials, rather than in averages over multiple trials. A variety of information theoretic quantities are commonly used in neuroscience - (see entry "Definitions of Information-Theoretic Quantities"). In this entry we review some applications of information theory in neuroscience to study encoding of information in both single neurons and neuronal populations.Comment: 8 pages, 2 figure

DFR Perturbative Quantum Field theory on Quantum Space Time, and Wick Reduction

We discuss the perturbative approach a` la Dyson to a quantum field theory with nonlocal self-interaction :phi*...*phi:, according to Doplicher, Fredenhagen and Roberts (DFR). In particular, we show that the Wick reduction of non locally time--ordered products of Wick monomials can be performed as usual, and we discuss a very simple Dyson diagram.Comment: 15 pages, pdf has active hyperlinks. To appear in the proceedings of the conference on "Rigorous quantum Field Theory", held at Saclay on July 19-21, 2004, on the occasion of Jacques Bros' 70th birthda

Spontaneous neoplasia in lions (Panthera leo): combined retrospective case series and literature review

Introduction: Lions (Panthera leo) are a key charismatic zoo species, but the scientific literature concerning neoplastic disease is scattered with limited reviews.Materials and methods: One-hundred-and-forty neoplasms from 133 lions (0.5 to 26 years old) were reviewed from IZVG Pathology, Easter Bush Pathology and current scientific literature (31 peer-reviewed publications, using Google Scholar/Pubmed keywords: “Panthera leo”, “tumor”, and “neoplasia”).Results: In 61.7% of individuals, neoplasia led to death or was life-threatening without treatment. 39.8% of all tumours metastasized. Primary neoplasms most frequently arose from mammary glands (17.1%), skin (15.7%) and lymph nodes or spleen (13.6%). Metastasized organs included liver (24.3%), lymph nodes (20.0%), spleen (15.0%), and lung (14.3%). Malignant/benign tumours comprised 76.4%/23.6%, respectively. Mammary gland adenocarcinoma [MGA] (15.7%) and lymphoma (15.0%) were most common. Skin (29% fibrosarcoma, 29% epithelial, 20% melanocytic, 10% piloleiomyosarcoma), parathyroid gland (adenoma), gallbladder (adenocarcinoma/adenoma: 60%/40%), intestines (adenocarcinoma), mammary gland (all malignant epithelial), testes (germ cell/stroma) and bone (osteosarcoma/myeloma) exhibited only primary tumours. Mean ages of specified African (n = 50), white African (n = 6) and Asian lions (n = 19) were 16, 12 and 12.6 years, respectively. Barbary lions (n = 3) were all >16 years old. Females dominated sex ratios in African (62%), and white African populations (100%). By subspecies the commonest tumours were: African: MGA (27.3%); white African: cutaneous mesenchymal tumours (57.1%); and Asians: haemangiosarcoma (42.1%). Three Barbary lions exhibited respectively MGA (pulmonary/hepatic/splenic/renal metastasis), uterine adenocarcinoma (pulmonary metastasis) and splenic erythrophagocytic T-cell lymphoma (nodal metastasis).Conclusions: Metastasizng malignant neoplasms of mammary gland and lymphoid origin are dominant in lions (>12 years)

Second harmonic generating (SHG) nanoprobes for in vivo imaging

Fluorescence microscopy has profoundly changed cell and molecular biology studies by permitting tagged gene products to be followed as they function and interact. The ability of a fluorescent dye to absorb and emit light of different wavelengths allows it to generate startling contrast that, in the best cases, can permit single molecule detection and tracking. However, in many experimental settings, fluorescent probes fall short of their potential due to dye bleaching, dye signal saturation, and tissue autofluorescence. Here, we demonstrate that second harmonic generating (SHG) nanoprobes can be used for in vivo imaging, circumventing many of the limitations of classical fluorescence probes. Under intense illumination, such as at the focus of a laser-scanning microscope, these SHG nanocrystals convert two photons into one photon of half the wavelength; thus, when imaged by conventional two-photon microscopy, SHG nanoprobes appear to generate a signal with an inverse Stokes shift like a fluorescent dye, but with a narrower emission. Unlike commonly used fluorescent probes, SHG nanoprobes neither bleach nor blink, and the signal they generate does not saturate with increasing illumination intensity. The resulting contrast and detectability of SHG nanoprobes provide unique advantages for molecular imaging of living cells and tissues

Full oxide heterostructure combining a high-Tc diluted ferromagnet with a high-mobility conductor

We report on the growth of heterostructures composed of layers of the high-Curie temperature ferromagnet Co-doped (La,Sr)TiO3 (Co-LSTO) with high-mobility SrTiO3 (STO) substrates processed at low oxygen pressure. While perpendicular spin-dependent transport measurements in STO//Co-LSTO/LAO/Co tunnel junctions demonstrate the existence of a large spin polarization in Co-LSTO, planar magnetotransport experiments on STO//Co-LSTO samples evidence electronic mobilities as high as 10000 cm2/Vs at T = 10 K. At high enough applied fields and low enough temperatures (H < 60 kOe, T < 4 K) Shubnikov-de Haas oscillations are also observed. We present an extensive analysis of these quantum oscillations and relate them with the electronic properties of STO, for which we find large scattering rates up to ~ 10 ps. Thus, this work opens up the possibility to inject a spin-polarized current from a high-Curie temperature diluted oxide into an isostructural system with high-mobility and a large spin diffusion length.Comment: to appear in Phys. Rev.

Complement C5a Functions as a Master Switch for the pH Balance in Neutrophils Exerting Fundamental Immunometabolic Effects

During sepsis, excessive activation of the complement system with generation of the anaphylatoxin C5a results in profound disturbances in crucial neutrophil functions. Moreover, because neutrophil activity is highly dependent on intracellular pH (pHi), we propose a direct mechanistic link between complement activation and neutrophil pHi In this article, we demonstrate that in vitro exposure of human neutrophils to C5a significantly increased pHi by selective activation of the sodium/hydrogen exchanger. Upstream signaling of C5a-mediated intracellular alkalinization was dependent on C5aR1, intracellular calcium, protein kinase C, and calmodulin, and downstream signaling regulated the release of antibacterial myeloperoxidase and lactoferrin. Notably, the pH shift caused by C5a increased the glucose uptake and activated glycolytic flux in neutrophils, resulting in a significant release of lactate. Furthermore, C5a induced acidification of the extracellular micromilieu. In experimental murine sepsis, pHi of blood neutrophils was analogously alkalinized, which could be normalized by C5aR1 inhibition. In the clinical setting of sepsis, neutrophils from patients with septic shock likewise exhibited a significantly increased pHi These data suggest a novel role for the anaphylatoxin C5a as a master switch of the delicate pHi balance in neutrophils resulting in profound inflammatory and metabolic changes that contribute to hyperlactatemia during sepsis