Glutathione increase by the n-butanoyl glutathione derivative (GSH-C4) inhibits viral replication and induces a predominant Th1 immune profile in old mice infected with influenza virus

During aging, glutathione (GSH) content declines and the immune system undergoes a deficiency in the induction of Th1 response. Reduced secretion of Th1 cytokines, which is associated with GSH depletion, could weaken the host defenses against viral infections. We first evaluated the concentration of GSH and cysteine in organs of old mice; then, the effect of the administration of the N-butanoyl GSH derivative (GSH-C4) on the response of aged mice infected with influenza A PR8/H1N1 virus was studied through the determination of GSH concentration in organs, lung viral titer, IgA and IgG1/IgG2a production and Th1/Th2 cytokine profile. Old mice had lower GSH than young mice in organs. Also the gene expression of endoplasmic reticulum (ER) stress markers involved in GSH metabolism and folding of proteins, i.e. Nrf2 and PDI, was reduced. Following infection, GSH content remained low and neither infection nor GSH-C4 treatment affected Nrf2 expression. In contrast, PDI expression was upregulated during infection and appeared counterbalanced by GSH-C4. Moreover, the treatment with GSH-C4 increased GSH content in organs, reduced viral replication and induced a predominant Th1 response. In conclusion, GSH-C4 treatment could be used in the elderly to contrast influenza virus infection by inducing immune response, in particular the Th1 profile

Alien Registration- D\u27Onofria, Celestino (Millinocket, Penobscot County)

https://digitalmaine.com/alien_docs/7432/thumbnail.jp

Cumulant-cumulant relations in free probability theory from Magnus' expansion

Relations between moments and cumulants play a central role in both classical and non-commutative probability theory. The latter allows for several distinct families of cumulants corresponding to different types of independences: free, Boolean and monotone. Relations among those cumulants have been studied recently. In this work we focus on the problem of expressing with a closed formula multivariate monotone cumulants in terms of free and Boolean cumulants. In the process we introduce various constructions and statistics on non-crossing partitions. Our approach is based on a pre-Lie algebra structure on cumulant functionals. Relations among cumulants are described in terms of the pre-Lie Magnus expansion combined with results on the continuous Baker-Campbell-Hausdorff formula due to A. Murua

Validation and optimization of the GSAMQ analytical propagator for spin-stabilized satellite: Validação e otimização do propagador analítico GSAMQ para satélite spin-stabilizado

The aim of this paper is to validate the analytical propagator through simulations comparing the results with the real data and to optimize the work's source code so that it is processed faster. This work analyzes the use of the quadrupole model in the Earth's magnetic field when an analytical propagator calculates eddy current and residual magnetic torques in spin-stabilized satellites. For that, the components of the gravity gradient torque, the solar radiation torque and aerodynamics torque are also included. The simulations are carried out for a predetermined period and use data from the SCD1 Brazilian satellite, provided by the National Institute for Space Research (INPE). The results are then compared with the real data to validate the propagator. The propagator with the quadrupole model is called GSAMQ and uses the mean deviations of the magnitude of the spin velocity, and the angles of right ascension and declination of the spin axis as evaluation parameters. A statistical approach is included in this analysis and shows the model's accuracy

Soluble P-selectin levels in synovial fluid and serum from patients with psoriatic arthritis

Objective: P-selectin is an adhesion molecule expressed by activated endothelial cells and platelets favouring the leukocyte adherence to microvascular endothelium. A soluble form of this molecule has been described, whose serum levels were found to be elevated and correlate with disease activity in rheumatoid arthritis (RA) patients. Aim of this study was to determine soluble P-selectin levels in synovial fluid (SF) and serum from patients with psoriatic arthritis (PsA), where it has never been investigated, to define its involvement in PsA synovial damage. Methods: we analysed, by ELISA, soluble P-selectin serum and SF levels in 100 patients presenting a knee joint effusion: 38 of them presented PsA, 40 RA and 22 osteoarthritis (OA). We examined the main clinical and laboratory parameters of these patients. Soluble P-selectin serum levels were also detected in 15 healthy subjects. Results: soluble P-selectin SF levels were significantly higher in PsA and RA patients respect to OA subjects. Soluble P-selectin SF levels were lower than those found in serum and the SF/serum ratio was higher in PsA and RA patients respect to OA. Soluble P-selectin serum levels were not significantly different among patients and controls. No correlation was found between SF and serum levels of soluble P-selectin and the main clinical parameters. Conclusions: our study of soluble P-selectin in PsA reveals a prominent local role of this molecule, with no differences respect to RA. Histological findings may be of help in understanding the role of this adhesion molecule in PsA

Caracterização físico-química e produtividade de grãos de cafeeiros do Banco de Germoplasma da Embrapa Cerrados.

O objetivo deste trabalho foi avaliar cafés arábica e canéfora por meio da caracterização físico-química e produtividade de grãos. Os 30 acessos de Coffea arabica L e os 22 clones de Coffea canephora foram caracterizados quanto à produtividade de grãos no ano de 2017; análises físico-químicas dos teores de ácido clorogênico (5-CQA), de cafeína (CAF), de açúcares solúveis totais (AST), de sólidos solúveis totais (SS) e de perfil de ácidos carboxílicos foram realizadas para os cafés arábica; para os cafés canéfora, foram determinados SS, AST e acidez titulável (AT). Os cafés arábica com maiores produtividades apresentaram valores médios acima de 100 sacas/ ha.bitstream/item/199839/1/Bolpd-345.pd

Epidemic spread of ST1-MRSA-IVa in a neonatal intensive care unit, Italy.

BACKGROUND: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has recently emerged as an important pathogen in neonatal intensive care units (NICUs). The purposes of this study were to characterize methicillin-resistant isolates from an outbreak in a NICU, to examine the genetic traits and clonality of CA-MRSA, and to review the characteristics and outcomes of the neonatal cases and investigate the routes of entry and transmission of the MRSA outbreak strain in the NICU under study. METHODS: The study NICU practiced an active surveillance program for multidrug-resistant organisms, including weekly cultures for detection of MRSA from nasal swabs among all the admitted neonates. All first isolates from surveillance cultures and all clinical isolates were submitted for susceptibility testing and genotyping. Data from each infant’s medical records were prospectively included in a database, and the clinical features and outcomes of the colonized/infected infants were assessed. RESULTS: A total of 14 infants were colonized or infected by a strain of ST1-MRSA-IVa between April and August 2011. The CA-MRSA strain appeared to have been introduced to the NICU by an infected infant transferred from another hospital. The outbreak was successfully contained by multifaceted infection control interventions. CONCLUSIONS: The results of this study confirm that NICU is a healthcare setting with a critical permeability to CA-MRSA. Active surveillance including molecular typing can help to detect and monitor the spread of antimicrobial drug-resistant organisms, and thus trigger timely control interventions

Panton-Valentine leukocidin positive sequence type 80 methicillin-resistant Staphylococcus aureus carrying a staphylococcal cassette chromosome mec type IVc is dominant in neonates and children in an Algiers hospital

Methicillin-resistant Staphylococcus aureus (MRSA) is a major antimicrobial drug-resistant pathogen causing serious infections. It was first detected in healthcare settings, but in recent years it has also become disseminated in the community. Children and young adults are most susceptible to infection by community-acquired (CA) MRSA strains. In this study 25 MRSA isolates implicated in infections of neonates and children admitted to an Algiers hospital during an 18 month period were characterized by molecular methods including staphylococcal cassette chromosome (SCC) mec typing, PCR amplification of pvl genes, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Fifteen out of 25 isolates were from hospital-acquired infections. Twenty-four isolates carried SCCmec type IVc and belonged to the sequence type (ST) 80, one isolate carried SCCmec type II and was ST 39. Twenty-two out of 24 ST80-MRSA-IVc isolates carried pvl genes. Our results suggest that the Panton-Valentine leukocidin positive ST80- MRSA-IVc is the dominant MRSA clone causing disease in neonates and children in Algiers