The Effect of stem cell mobilisation with granulocyte colony-stimulating factor on the morphology of the haematopoietic organs in mice

The cellular mobilisation of mice with granulocyte colony-stimulating factor (G-CSF) results in an egress of haematopoietic stem/progenitor cells from the bone marrow and an increase in their level in the peripheral blood. While the mobilisation process with different agents is widely studied, little is known about the morphology of the murine haematopoietic organs during the mobilisation. The purpose of this study was to examine the morphology of the bone marrow, spleen and liver in mice mobilised with G-CSF. To address this issue mice were injected subcutaneously with G-CSF for 6 consecutive days. Morphological analysis revealed an increase in the number of mature neutrophils close to the wall of sinusoids in the bone marrow as well as hypertrophy of the red pulp in the spleen. At the same time no morphological changes were noticed in the livers of G-CSF-mobilised mice. In conclusion, G-CSF induces discrete ultrastructural changes in the bone marrow, which intensify the transendothelial traverse of haematopoietic stem and progenitor cells from it. The changes in the spleen are related to repopulation of this organ by mobilised early haematopoietic cells circulating in the peripheral blood. We also noticed that the process of migration of haematopoietic cells from the bone marrow into the peripheral blood began on day 2 and was most pronounced on day 4 after stimulation with G-CSF

Effect of Neutron Irradiation on the EPR Spectra of CuGeO3\text{}_{3}

The influence of neutron irradiation on the electron paramagnetic resonance spectra of the spin-Peierls compound CuGeO$\text{}_{3}$ was investigated in the wide temperature range 2-300 K. It was found that the irradiation induced appreciable changes in the EPR signal intensity, resonance line width and g-factor of this material. It was also shown that the irradiation leads to a decrease in the spin-Peierls transition temperature. For higher irradiation doses, an antiferromagnetically ordered phase can be observed at lower temperatures. This phase coexists with the spin-Peierls phase. The observed decrease in the spin-Peierls transition temperature after irradiation may be associated with some changes in topological and chemical short-range order or with the transmutation of the fraction of $\text{}^{63}$Cu in the $\text{}^{63}$Cu(n,α)$\text{}^{60}$Co reaction

Effect of Heavy Ion Irradiation on the Magnetic Properties of CuGeO3\text{}_{3}

The influence of heavy ion irradiation on the magnetic properties of the spin-Peierls compound CuGeO$\text{}_{3}$ has been investigated by means of the electron paramagnetic resonance, the spontaneous magnetization, and the low field ac susceptibility. The measurements were performed on the CuGeO$\text{}_{3}$ single crystals before and after irradiation with 80 MeV oxygen ions in the wide temperature range of 2-300 K. It was found that the irradiation induced an increase in the EPR signal intensity, magnetization, and susceptibility of this material. It was also shown that the irradiation does not lead to a decrease in the spin-Peierls transition temperature and to the formation of an antiferromagnetic phase which is expected to appear in disordered spin-Peierls systems